Novel use of bamboo shell extract
A technology of bamboo shoot shells and extracts, which is applied in the fields of application, food science, and drug combination, and can solve problems such as limited effects, side effects, and clinical dosage limitations.
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Embodiment 1
[0013] Embodiment one, the preparation of bamboo shoot shell extract
[0014] Accurately weigh 0.50 kg of dry bamboo shoot shells, use a pulverizer to pulverize and reclaim the finely powdered bamboo shoot shells, add 8 times the amount of 95% ethanol, and extract at 80°C under reflux for 120 minutes. After the extract is filtered through gauze, the filter cake is then Extract twice under the same conditions, combine the filtrates, centrifuge at 3000 rpm for 15 minutes, recover the supernatant and freeze-dry. The above freeze-dried extract of bamboo shoot shells with a recovery of about 0.48-5.0% was used in the following examples of the present invention.
Embodiment 2
[0015] Embodiment 2, the improvement effect of bamboo shoot shell extract on restraint stress load mouse plasma alanine aminotransferase (ALT) activity
[0016] Plasma alanine aminotransferase activity is a characteristic indicator of the degree of hepatocyte injury. So far, studies have proved that stress response can cause a large number of free radicals and cytokines. For example, stress load activates macrophages in the liver, which in turn activates various cytokines and triggers an inflammatory response. In addition, in this reaction process, the cytotoxicity of lymphocytes and macrophages can also be induced, and liver cells can be damaged through various ways such as liver cell apoptosis. The assay principle we adopt is to use alanine aminotransferase (ALT) to act on the substrate composed of alanine and α-ketoglutarate at 37°C and pH 7.4 to generate pyruvate and glutamic acid. After reacting for a certain period of time, add 2,4-dinitrophenylhydrazine (DNPH) hydrochl...
Embodiment 3
[0018] Embodiment three, the improvement effect of bamboo shoot shell extract on restraint stress load mouse plasma antioxidant capacity index (ORAC)
[0019] Antioxidant capacity index (ORAC), as a relatively systematic method that can comprehensively and objectively reflect the antioxidant level of substances, has been more and more recognized and widely used at home and abroad (Chinese Pharmacology Bulletin, 22: 1015-1021, 2006). The principle is that sodium fluorescein emits 527nm fluorescence under the excitation of 485nm light, which can be oxidized by free radicals released by AAPH to make the fluorescence properties disappear. When antioxidants exist, they can compete with sodium fluorescein for oxidants and slow down the rate of fluorescence fading. According to this characteristic, it can be used to determine the oxygen free radical scavenging activity of the sample.
[0020] In this experiment, 6-week-old male Kunming mice purchased from the Guangdong Provincial M...
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