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Applications of black rice anthocyanin extract in preparing medicament for protecting restraint stress-induced liver injury

A technology of anthocyanins and extracts, applied in the field of medicine

Inactive Publication Date: 2013-11-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new application of black rice anthocyanin extract to solve the defects of existing drugs for protecting restraint-induced stress-induced liver injury

Method used

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  • Applications of black rice anthocyanin extract in preparing medicament for protecting restraint stress-induced liver injury
  • Applications of black rice anthocyanin extract in preparing medicament for protecting restraint stress-induced liver injury
  • Applications of black rice anthocyanin extract in preparing medicament for protecting restraint stress-induced liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Preparation of black rice anthocyanin extract

[0026] Step (1): crush the black rice, add 8 times the amount of 40% ethanol containing 1% hydrochloric acid to the black rice powder, shake and extract at 30°C for 2 hours, and collect the filtrate by suction filtration;

[0027] Step (2): the black rice flour filter residue in the step (1) is extracted by shaking with 40% ethanol containing 1% hydrochloric acid in the same volume for 2 hours, and the filtrate is collected by suction filtration;

[0028] Step (3): combining the filtrate in step (1) and step (2), and concentrating under reduced pressure to remove ethanol;

[0029] Step (4): Put the concentrate of step (3) on AB-8 macroporous resin, after the anthocyanins are completely absorbed, wash the column with 3 times column volume of distilled water containing 1% hydrochloric acid, and then use 80% hydrochloric acid containing 1% hydrochloric acid to clean the column. % ethanol analysis anthocyanins; ...

Embodiment 2

[0031] Embodiment 2: Preparation of black rice anthocyanin extract

[0032] Step (1): Pulverize the black rice, add 55% ethanol containing 1% hydrochloric acid in 8 times the amount of black rice flour, shake and extract at 25°C for 3 hours, and collect the filtrate by suction filtration;

[0033] Step (2): the black rice flour filter residue in step (1) is extracted with the same volume of 55% ethanol containing 1% hydrochloric acid concussion for 3 hours, and the filtrate is collected by suction filtration;

[0034] Step (3): combining the filtrate in step (1) and step (2), and concentrating under reduced pressure to remove ethanol;

[0035] Step (4): Put the concentrated solution of step (3) on D101 macroporous resin. After the anthocyanins are completely absorbed, wash the column with 5 times column volume of distilled water containing 1% hydrochloric acid, and then use 65% ethanol containing 1% hydrochloric acid Analysis of anthocyanins;

[0036] Step (5): The anthocyan...

Embodiment 3

[0037] Example 3: Protective effect of black rice anthocyanin extract on restraint-induced stress liver injury in mice

[0038] Materials and reagents: Alanine transaminase (GPT) kit (100T / 50 samples, Nanjing Jiancheng Bioengineering Research Institute), reduced glutathione (GSH) kit (specification: 100T / 96 samples, Nanjing Jiancheng Bioengineering Research Institute) Institute), malondialdehyde (MDA) test box (specification: 100T / 96 samples, Nanjing Jiancheng Bioengineering Research Institute), xanthine oxidase (XOD) test box (specification: 50T / 48 samples, Nanjing Jiancheng Bioengineering Research Institute ); absolute ethanol (Jiangsu Qiangsheng Functional Chemical Co., Ltd.), concentrated hydrochloric acid (Kunshan Jincheng Reagent Co., Ltd.), glacial acetic acid (Sinopharm Chemical Reagent Co., Ltd.).

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Abstract

The invention discloses applications of black rice anthocyanin extract in preparing medicament for protecting restraint stress-induced liver injury. The related research shows that the black rice anthocyanin extract achieves the function of obviously balancing the reactive oxygen species in the body, is capable of reducing the GPT (Glutamic Pyruvic Transaminase), MDA (Methylene Dioxyamphetamine) and XOD (Xanthine Oxidase) levels of the organism under the pathological condition, and improving the GSH (glutathione) level of antioxidant substance in the body, and can be used for preparing the medicament for protecting restraint stress-induced liver injury.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of black rice anthocyanin extracts in the preparation of medicines for protecting liver damage. Background technique [0002] In recent years, oxidative stress diseases have attracted more and more attention. The body produces a large number of active oxygen free radicals under stress load. When these free radicals cannot be removed in time and accumulate in the body, they will trigger lipid peroxidation, destroy the structure and function of cell membranes, and cause tissue lesions. The restraint stress model is a classic psychological stress model. Restraint induces stress free radical reactions in the body, thereby promoting the production of harmful substances in cell membranes, proteins, enzymes and DNA; restraint stress can also reduce the body's immunity and affect the secretion of hormones in the body , Promote the production of lipid peroxides and reduce the lev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61P1/16
Inventor 李贺然郝杰祝慧李彦敏
Owner SUZHOU UNIV
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