Application of glabrous sarcandra glabrous herb extract in reducing susceptibility of influenza virus

An influenza virus and extract technology, applied in the field of medicine, can solve problems such as effective protection and no intellectual property rights

Active Publication Date: 2014-07-30
GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has been no report on the study of Jiujie tea extract in reducing the body's susceptibility to influenza virus, and there is no report on the application of Jiujie tea extract in preventing, alleviating and treating the susceptibility and complications of influenza virus. Effective Protection of Intellectual Property Rights

Method used

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  • Application of glabrous sarcandra glabrous herb extract in reducing susceptibility of influenza virus
  • Application of glabrous sarcandra glabrous herb extract in reducing susceptibility of influenza virus
  • Application of glabrous sarcandra glabrous herb extract in reducing susceptibility of influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1. The preparation method of Jiujie tea extract

[0011] Take 10 kg of dried Jiujie tea medicinal materials, add water to decoct 2 times after sorting, add 10 times the amount of water to decoct for 2 hours each time, filter the decoction, combine the filtrates twice, concentrate the filtrate into an extract, add ethanol while stirring, Stand still, centrifuge to get the supernatant, then add ethanol, the precipitate obtained after standing still is vacuum-dried, and the extract of Jiujie tea obtained after vacuum drying is used in this study. In view of the fact that Jiujie tea extract is developed with medicine or medicine composition as the final product, it is advisable to choose drinking water, ethanol or hydrous ethanol as the extraction solvent from the perspective of safety. The present invention has no specific limitation on the resting time of the alcohol precipitation, and the extraction methods can be heating extraction, ultrasonic extraction, microw...

Embodiment 2

[0012] Example 2: Effects of Jiujie tea extract on the morbidity and mortality of stress-loaded mice infected with viruses

[0013] The experimental animals were male SPE-grade 4-week-old Kunming mice (KM mice) weighing 13-16 g, which were purchased from Guangdong Medical Experimental Animal Center, license number SCXK (Guangdong) 2008-0002. Mice feed was purchased from Guangdong Medical Experimental Animal Center. The experimental animals were raised in a clean-grade laminar flow frame, the ambient temperature was air-conditioned at 23±2°C, the relative humidity was 75±10%, and the lighting time was 12h / d (7:00-19:00). Experimental animals were randomly divided into normal group, virus group, model group (restraint+virus), positive drug group (restraint+virus+ribavirin, 50mg / kg / day), low-dose Jiujie tea extract group (restraint+ Virus + Jiujie tea extract, 250mg / kg / day), Jiujie tea extract high dose group (restraint + virus + Jiujie tea extract, 500mg / kg / day), 9 to 11 rats i...

Embodiment 3

[0024] Example 3: Determination of the amount of virus in the lung tissue of infected stress-loaded mice by Jiujie tea extract

[0025] According to the viral nucleoprotein NP mRNA level in the lung tissue represents the amount of virus in the lung tissue, we grouped the experimental animals, restrained stress, infected with influenza virus and administered the same as in Example 2. On the 5th day after the stress mice were infected with the virus, they were sacrificed under ether anesthesia, and the lungs were removed to extract RNA to measure the expression of NP mRNA. The experimental data are expressed as Mean±SD, SPSS 19.0 software is used for data processing, and ANOVA test is used for statistical analysis, p<0.05 is considered statistically significant. The experimental results are shown in Table 3. The amount of virus in the lungs of restraint-stressed mice increased significantly, and the administration of Jiujie tea extract (250, 500mg / kg / day) could significantly red...

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Abstract

The invention provides novel application of a glabrous sarcandra glabrous herb extract, belongs to the field of medicines, and provides an active extract of a medicinal material glabrous sarcandra glabrous herb through water extraction, alcohol precipitate and drying. 100g of the extract comprise 5 to 20g of tannin, and 0.02 to 1g of isofraxidin. The extract is confirmed and proved due to the effect that the incidence rate and death rate of a low influenza A H1N1 virus FM1 strain infected restraint stress loaded mouse can be reduced based on the glabrous sarcandra glabrous herb extract, and the lung tissue viral load of an influenza virus loaded mouse and inflammatory factor effects can be remarkably reduced by the extract. The glabrous sarcandra glabrous herb extract can be used for preparing medicaments applied to susceptibility of a human body to the influenza virus, relieving pneumonia and other respiratory inflammations due to secondary of body susceptible virus, pulmonary dysfunction and other clinical symptoms, and can be used for particularly effectively preventing, reducing or treating various clinical symptoms caused by virus injection occurring in an influenza virus susceptible body.

Description

technical field [0001] The invention relates to the field of medicine, is a field of natural plant extracts and applications thereof, and relates to a new application of Jiujie tea extract for reducing the susceptibility of a body to influenza virus. Background technique [0002] Influenza is one of the major public health problems faced by mankind, which seriously threatens human health. Influenza viruses can spread through the air, causing complications such as respiratory infections, viral pneumonia, bronchitis, and even death. In fact, when people face the attack of influenza virus, they show great differences in organisms. This is because the pathogenesis of influenza is affected by two factors: influenza virus and host. Therefore, finding drugs that improve and reduce the susceptibility of the body to influenza virus has important clinical significance in the prevention and treatment of virus infection. [0003] It is known that with the transformation of modern life...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P11/00A61P31/16
Inventor 何蓉蓉曹会娟李怡芳栗原博严志标彭红英陆颂规江涛陈雪华
Owner GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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