Hematoxylin-eosin one-step dyeing method
A technology of hematoxylin and staining, applied in the field of hematoxylin-eosin staining solution and its staining, can solve the problems of shortening operation time, difficulty in grasping the degree of staining of nucleus and cytoplasm, etc., and achieves easy observation and analysis, good staining effect and stable results Effect
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[0019] Taking conventional formalin fixation and paraffin-embedded pathological tissue sections as an example, the conventional HE staining and the operation steps of the present invention are introduced as follows.
[0020] Operation steps of conventional HE staining method.
[0021] 1. Dewax the slices to water.
[0022] ① Xylene acts on 2 times, 5-10min each time.
[0023] ②The anhydrous ethanol is used for 2 times, 3~5min each time.
[0024] ③95% ethanol for 3 to 5 minutes.
[0025] ④ 90% ethanol for 3 to 5 minutes.
[0026] ⑤ 80% ethanol for 3 to 5 minutes.
[0027] ⑥ Rinse with tap water or distilled water for 1 to 3 minutes.
[0028] 2. HE staining.
[0029] ①Stain with modified Lillie-Mayer hematoxylin staining solution for 1 to 5 minutes.
[0030] ② Rinse with tap water or distilled water for 10-30s.
[0031] ③Differentiate with 1% hydrochloric acid and ethanol for 2 to 5 seconds.
[0032] ④ Rinse with tap water or distilled water for 10-30s.
[0033] ⑤ Weakly alkaline water (such as 0....
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