101 results about "Lymph Gland" patented technology

The invention discloses a stomach computed tomography (CT) sequence image segmentation method based on interactive region growth, which mainly solves the problems that in the prior art, CT sequence segmentation speed is slow, and poor segmentation is easy to occur. The method includes: firstly, a seed point is selected manually in a target area to be segmented in a first image, the interactive region growth is used for performing segmentation, a center of a segmentation result and eight neighborhoods of the center are projected into a next CT image to serve as seed points, the interactive region growth is continuously used for performing segmentation to obtain the target area of the current image, and the segmentation result of the previous image is projected into a next image repeatedly to serve as a seed point to be segmented continuously until segmentation of a whole sequence is completed. Compared with a traditional serial region growth, the stomach CT sequence image segmentation method based on the interactive region growth has the advantages of being rapid in speed, good in effect and the like, can be used for segmenting stomach CT sequence images, and can well segment target areas which may occur in stomach lymph gland in the sequence.

The invention provides an MRI image-based axillary lymph gland metastasis prediction system and relates to the technical field of computer aided diagnosis. The system comprises an input module, an area-of-interest extraction module, a lump segmentation module, a sub-visualization module, a feature extraction module, a feature dimensionality reduction module, a classification and diagnosis module,and an output module. The input module receives a to-be-diagnosed mammary gland DCE-MR image sequence input by a user. The area-of-interest extraction module extracts an area of interest from the mammary gland DCE-MR image sequence. The lump segmentation module segments a lump in the area of interest. The sub-visualization module carries out the visual display on each segmented image and extractsthe edge of a focus. The feature extraction module extracts relevant feature values according to the lump information and transmits the relevant feature values to the feature dimensionality reductionmodule. The feature dimensionality reduction module carries out feature dimensionality reduction on an extracted feature set. The classification and diagnosis module inputs each lump feature value into a classifier. After that, the automatic classification and recognition is carried out by a computer for judging whether a lymph gland has already been transferred or not. The output module displaysa transfer prediction result and a transfer probability. According to the invention, the accurate segmentation of breast lesions can be realized. The accurate diagnosis of mammary axillary lymph glandmetastasis can be effectively assisted.

The invention discloses a computer aided gastric cancer diagnosis method based on target tracking and mainly solves the problem of lymph gland transfer judging in a gastric cancer diagnosis process in the field of medical imaging. The steps include 1 segmenting images; 2 extracting tracked targets; 3 forecasting tracking; 4 judging whether all target forecasting tracking is finished, if all target forecasting tracking is finished, a next step is performed, otherwise, the step 3 is returned; 5 matching features; and 6 identifying lymph gland. A method of computer aided medical diagnosis is applied to detection of gastric cancer lymph gland transfer, a large amount of stomach section images can be processed in a short period of time, detecting speed and accuracy rate are improved compared with traditional detection of gastric cancer lymph gland transfer, automatic identification of lymph gland of stomach section images is achieved, clinical diagnosis time is greatly shortened, and a better treatment opportunity is obtained for patients.

The present invention relates to a fluorescence navigation system used in tumor surgery, which belongs to clinical medicine surgery. The present invention solves problems existing in the surgical excision operation process of tumors that tumor boundaries are difficult to identify, remnant tumor tissue is difficult to recognize, and tumor metastasis lymph glands are difficult to track. The fluorescence navigation system used in tumor surgery is characterized in that the system comprises a biofluorescence excitation light source, a light guide system, a specificity targeted marking fluorescent material, a video camera and an image display and processing device connected with the video camera. The present invention provides fluorescence navigation concept for tumor surgery based on the prior art. When physicians identify tumor boundaries, recognize remnant tumor tissue, and track tumor metastasis lymph glands with a fluorescence observing mirror or a camera display system, tumor tissue is thoroughly excited, operation therapeutic effect is effectively improved, and the death rate of tumor patients is reduced. The present invention makes a breakthrough in tumor surgery.

The invention provides a double FQ-PCR detection kit for identifying PCV (porcine circoviruses) type 2 and type 3. By aiming at the characteristic of low Rep protein and Cap protein gene homology of PCV2 and PCV3, a pair of specific primers and a TaqMan MGB probe (SEQ ID NO:1-6) are designed by using Rep protein gene of PCV2 and Cap protein gene of PCV3 as amplification target regions; the real-time fluorescence quantitative PCR technology is used; the identification detection of the PCV2 and the PCV3 is realized. The detection kit provided by the invention is applicable to viral nucleic aciddetection in samples such as nose swab, oral cavity secreta, faeces, serum, lungs, lymph gland and kidneys of a suspected PCV infectious pig; the sensitivity can reach 1.0*10<1> copy/mu L; the crossedreaction with other pathogene capable of generating mixed infection or with similar of infection symptoms do not exist.

The invention provides a manganese dioxide nanometer material. The manganese dioxide nanometer material comprises manganese dioxide nanoparticles and carried matter, wherein the carried matter is compounded onto the surface of the manganese dioxide nanoparticle; the carried matter comprises oligonucleotide CpG and/or antigen; the antigen comprises protein antigen and/or polypeptide antigen. The provided manganese dioxide nanometer material has the advantages that the immunostimulation function of an immunologic adjuvant CpG is greatly improved, the immunity protection function of vaccine is improved, and the inherent defect of effective improving of immune reaction by a large amount of CpG because the oligonucleotide CpG is used as an immunologic stimulant and cannot easily enter the body lymph glands is solved; the manganese dioxide nanometer material is used as a vaccine adjuvant, and can carry the antigen, especially the protein antigen, so that the intake on the antigen by the immune cells in the lymph glands is effectively improved, and the immunogenicity of the antigen is improved; the nanometer adjuvant can be biologically degraded, and can be gradually degraded into manganese ions (Mn<2+>) under the weak acid environment, such as lysosome, and the manganese ions are finally discharged out of the human body.

Methods for diagnosing prostate cancer, and differentiate prostate cancer from other prostate complications, and use of said method, and diagnosing and monitoring lymph gland metastasis, post operative examinations, and examinations during or after radiation, cytostatic, and androgen treatments are disclosed. The methods comprise injecting tracer-labelled PSA or hK2 specific antibodies, visualising PSA or hK2 producing tissue with the aid of a visualisation method, and diagnosing prostate cancer from the difference in visualisation.

The invention discloses an activated carbon nanosphere which is prepared by taking activated carbon particles with the particle sizes of 10-100nm as carriers, and the surfaces of the activated carbon particles are compounded with an organic dyestuff. The activated carbon nanosphere disclosed by the invention is rather strong in dyeing property, remarkable in tracing effect and capable of remarkably showing a lymph tracing effect for a tested living body within half a hour, realizing multi-mode imaging and directly observing the trace of the nanosphere from tumors, tissues or organs through naked eyes.

The invention relates to a traditional Chinese medicine composition for treating pulmonary tuberculosis, which comprises the following components of lilium brownie, ladybell root, lilyturf root, rehmannia, ginseng, cortex lycii, bulbus fritillariae cirrhosae, radix stemonae,common bletilla pseudobulb, radix astragali, Chinese yam and radix ranunculi ternati. The traditional Chinese medicine composition has the effect of nourishing yin for moistening lung, replenishing qi and invigorating the spleen, and can be used for preparing a medicine for preventing and treating pulmonary tuberculosis and/or lymph gland tuberculosis and/or tuberculous pleurisy. The invention also relates to a preparation method of the traditional Chinese medicine composition, which adopts different extraction methods according to the medicine properties of different herbal medicines to extract effective components in the herbal medicines maximally, thus enhancing medicine effect.

The medicine for treating bed sore and ulcer is prepared with frankincense, myrrh, pearl, fossil fragemts, borneol and other four kinds of Chinese medicinal materials and through decoction and other steps to form plaster to medicine medicine strip. It has high curative effect on bed sore, bone tuberculosis, lymph gland tuberculosis and various purulent ulcer.

Provided are a thyroid gland neck tissue classifying and identifying system and method based on an autofluorescence technology and an optical coherence tomography. The system organically integrates anautofluorescence system and an optical coherence tomography system, utilizes high-sensitivity tissue element resolution capability of the autofluorescence system and high-resolution tissue structureimaging of the optical coherence tomography system to achieve quick, identifier-free, nondestructive, high-sensitivity and real-time thyroid gland neck tissue classification and identification. By adopting the thyroid gland neck tissue classifying and identifying system and method, the classification and identification efficiency and accuracy of doctors to parathyroid glands, thyroid glands, fat,lymph glands and other neck tissues in clinic thyroid gland operation can be effectively improved, the doctors can be assisted to protect the parathyroid glands and thoroughly remove the lymph glands.

The invention provides a lymph gland targeted metastatic human hepatoma cell strain. The mother cell is a red fluorescent protein-expressed hepatoma cell HCCLM3-R. The human hepatoma cell strain is characterized in that the cell is named as lymph gland targeted metastatic human hepatoma cell strain HCCLM3-R-LnM1 according to the classified nomenclature with a preservation number of 3645, and has the following biological characteristics: the cell is polygonal epithelioid and is in adherent growth and loss of contact growth inhibition, is of a hypo-triploid karyotype, and has 50-58 of chromosomes each of which secretes AFP proteins. The establishing method comprises the following steps: using subcutaneous and liver orthotopic transplantation tumor models of a nude mouse to carry out spontaneous lymphatic metastasis screening, and then carrying out in-vitro cloning, amplification and purification on the lymphatic metastasis human hepatoma cell. The invention has wide application prospects and potential social and economic values in the basic research on hepatoma and the pre-clinic research on medicament.

The invention provides a preparation method, a formula and a use method of a bovine mycoplasma pneumonia inactivated vaccine. The preparation method of the bovine mycoplasma pneumonia inactivated vaccine comprises the following steps: 1, selecting diseased cattle which naturally infect mycoplasma pneumonia and is dying, taking diseased lungs, lymph glands and spleens to homogenize and removing precipitates and grease through filtering and centrifugation to obtain an antigen solution; 2, adding formaldehyde into the antigen solution to inactivate for 72 hours, adding 1,000 units of penicillin and 1,000 units of streptomycin into the antigen solution per millimeter and uniformly mixing to obtain an inactivated antigen solution; 3, mixing the inactivated antigen solution with an aluminum hydroxide adjuvant according to the proportion of 4:1 to obtain the inactivated vaccine. The vaccine prepared by the preparation method is safe in application, strong in pertinence and indeed in immune protection effect on mycoplasma causing bovine pleuropneumonia by infection, the lung lesion score of the infected cattle can be obviously reduced, the material weight ratio is improved, and the good effects of prevention and disease control can be achieved.

The invention provides a triplet fluorescence quantitative detection kit for PRRSV (Porcine reproductive and respiratory syndrome virus), HP-PRRSV (Highly pathogenic porcine reproductive and respiratory syndrome virus) and CSFV (Classical Swine Fever Virus). The triplet fluorescence quantitative detection kit comprises primers and probes listed in SEQ ID No.1-9 in a sequence table, as well as a total virus RNA extraction agent, an RT-QPCR mixed enzyme solution, RNase Free dH2O, and one or more of positive controls and negative controls. The kit is based on a triplet fluorescence quantitative PCR technology, PRRSV molecules, HP-PRRSV molecules and CSFV molecules are simultaneously detected, the kit is applied to detection of insignificant-content samples such as animal lungs, lymph glands, spleens, kidneys, hearts, livers, blood and meat products, whether the sample donors are contaminated or not is determined, and the kit is applicable to quarantine of live animals and meat products. The kit has the advantages of good specificity and high sensitivity, the detection cost is greatly lowered through joint detection, the detection time is saved, and the type of a pestilence can be conveniently determined in a short time at an epidemic outbreak.

The invention relates to the field of microbiological animal cells, in particular to a human hepatocarcinoma cell line with high lymph channel transfer potency established in lymph gland transfer stove, wherein nude mouse in vivo continuous screening method lymph channel transfer screening is employed to create human liver cancer cell line HCCLYM with high lymphatic channel transfer proficiency.

The invention relates to a medical use of engrafted nanometer carbon for preparing the target medicine for lymph labeling or directly killing transferred cancer cell in the lymph gland. The engrafted nanometer carbon is produced by surface modification, connecting nanometer carbon with hydrophilic group. The engrafted nanometer carbon can be dispersed into engrafted nanometer carbon suspension in water without dispersing auxiliary and grinding scattering. The suspension has a better lymph labeling and lymph target treatment effect, and has more surface active group and connects to other chemotherapy medicine. Therefore, it is possible to exploit the engrafted nanometer carbon to the lymph target chemotherapy medicine.

The invention relates to a test sample allergenicity detection method and a kit thereof. The detection method comprises the following steps: randomly dividing a plurality of mice into groups; measuring the thickness of ears of each mouse of different groups; entering into a first time period; within the first time period, smear an equal amount of a reagent on the ears of each mouse of different groups; entering into a second time period; when the second time period is ended, firstly measuring the thicknesses of the ears of the mice of different groups, further killing the mice, taking down lymph glands after the ears of the mice under a sterile condition, preparing a cell suspension, putting a 5-bromodeoxyuridine solution into the cell suspension, culturing, and testing the content of the5-bromodeoxyuridine by using an ELISA (Enzyme-Linked Immuno Sorbent Assay) method; and judging the allergenicity of a test substance according to limiting standards. By adopting the method, whether the test substance has positive allergenicity can be tested, intraperitoneal injection is avoided, then animals can be prevented from pain, the amount of the 5-bromodeoxyuridine can be greatly reduced,and the detection cost can be reduced.

The invention discloses application of gene HES2 to the auxiliary diagnosis, prognosis and treatment of esophageal squamous carcinoma. The HES2 gene is highly expressed in esophageal squamous carcinoma tissues. The experiment further discovers that mRNA of HES2 gene is highly expressed in an esophageal squamous carcinoma cell line, the progress of the HES2 high-expressed tumor is faster and is more likely to be transferred to lymph gland or far organs, which is significantly related to poor five-year disease-free survival rate and five-year general survival rate. In addition, an antisense oligonucleotide chain sequence targeting HES2 gene is also designed to silencing HES2 expression and can be useor the trd featment of esophageal cancer. HES2 can be used as a detection marker for assisting the diagnosis of the esophageal squamous carcinoma and predicting the reoccurrence trend of the esophageal squamous carcinoma as well as a treatment target spot.

The invention provides a mucosal adjuvant. The mucosal adjuvant is prepared by copolymerization crosslinking compounding of oligo-chitosan and lymphotactin encoding plasmids. The invention also discloses a preparation method and a use of the mucosal adjuvant. When the mucosal adjuvant and a gene vaccine composed of chitosan or oligo-chitosan are synchronously dropped into a nasal cavity for immunization, coxsackievirus specific serum antibody and systemic (spleen and lymph glands) Th1-type immune responses are induced and especially, intestinal mucosa partially-reinforced specific-secretion-type sIgA and IFNgama+Th1 responses are induced, and thus a gene vaccine used with the mucosal adjuvant is obviously superior to an exposed gene vaccine and effectively prevents coxsackievirus-caused myocarditis. The mucosal adjuvant can be used as a novel mucosal vaccination adjuvant.

The invention belongs to the technical field of pathological apparatus and discloses a positioning and marking system for finding lymph glands. The positioning and marking system for finding lymph glands solves the technical problems that the prior art depends on hands of the pathologist to find lymph glands, the finding time is long, the lymph gland number is insufficient, and the small lymph glands are easy to be neglected. According to the technical scheme, the positioning and marking system for finding lymph glands comprises an ultrasonic detecting device body, an imaging device, a probe, a positioning and marking device, an automatic identifying device, a mode selecting device, a wireless power supply receiving device, and an assorted wireless power supply emitting device connected with an external power supply. The positioning and marking system for finding lymph glands uses ultrasonic imaging principle and wireless design to realize observation, positioning and marking in real time, and the probe change, detecting and marking modes, observation angle regulation and positioning needle marking power can be decided according to actual situations; the positioning and marking system for finding lymph glands provides comprehensive and effective support for the later lymph gland shearing for the pathologist, has advantages of flexible use, convenience in carrying, comprehensive detection and time and labor saving, effectively reduces the workload of the pathologist and improves the working efficiency.

The invention provides a yew anti-cancer paste, which is an extract of more than ten rare traditional Chinese medicines including taxol, dragon's blood, oldenlandia diffusa and Chinese actinidia root. The external anti-cancer paste is prepared by ultrasonically crushing cytoplasm and coordinating with azone and magnetic chips. The paste has high drug loading capacity, drug factors of quantum particle peptides activate the magnetic memory of the drug factors due to the strong magnetic field function of the magnetic chips, the drug factors rapidly penetrate through the skin and enter the focus and penetrate through the cancer cells in a targeting mode, and the DNA protein chain polymerization is locked and is not depolymerized, so that the cancer cells atrophy, suicide and generate apoptosis. The paste is rapid in effect, high in air permeability, convenient and comfortable, has no toxic or side effect and constant-speed slow release, and has an extremely obvious treatment effect on cancers such as gastrointestinal carcinoma, lung cancer, breast cancer, skin cancer, nasopharynx cancer, laryngeal carcinoma, lymph gland cancer, superficial skin tumor and pancreatic cancer. The anti-cancer paste is simple in process, easy to operate, high in medicine activity and outstanding in antalgic anti-cancer effect.