32 results about "Tissue binding" patented technology

Fibroblast growth factor receptor (FGFR) extracellular domain (ECD) acidic region muteins that have been engineered to exhibit decreased tissue binding by increasing the number of acidic amino acid residues within the D1-D2 linker region are provided. Polynucleotides encoding FGFR ECD acidic region muteins are also provided. Methods of making FGFR ECD acidic region muteins, and methods of using such molecules to treat proliferative disorders, including cancers, disorders of angiogenesis, and macular degeneration, are also provided.

The present disclosure relates to the construction, expression, and selection of genes that encode novel Trp cage polypeptides with desirable cell and / or tissue binding properties, as well as the novel Trp cage polypeptides themselves. A polypeptide of this disclosure may contain all or part of amino acid sequence AAADX1YX2QWLX3X4X5GPX6SGRPPPX7 (SEQ ID NO: 4), wherein Xn represents an amino acid found in position n, the polypeptide comprising a tryptophan cage (Trp cage).

The present invention relates to a contrast-enhanced diagnostic imaging method for monitoring the efficacy of interventional therapies. The contrast agents useful in this method comprise an image-enhancing moiety (IEM) and a state-dependent tissue binding moiety (SDTBM). These contrast agents exhibit state-dependent binding to one or more components of a targeted tissue or tissue component and provide a detectable change in the signal characteristics of the agent once bound to the targeted tissue. As a result, these agents exhibit a binding affinity for, and thus image contrast of, the targeted tissue which changes as the tissue-state changes during therapy.

Surgeons may face the difficulty of preoperatively selecting the location for making an incision and intraoperatively identifying and differentiating targeted tissue for removal or for identification so that it is not removed along with neighboring or non-target tissue or so that neighboring or non-target tissue is not nicked, harmed, or removed accidentally. A tissue specific marker can include an aptamer or an affimer configured to bind to a pre-selected target tissue and one or more indicator elements coupled with the aptamer or the affimer. The one or more indicator elements produce a signal thereby allowing identification of the target tissue in a preoperative and operative manner.

The invention relates to contrast agents for medical science, in particular to a lymph targeted nuclear magnetic resonance imaging contrast agent with brown algal polysaccharide serving as a carrier and a preparation method and application of the lymph targeted nuclear magnetic resonance imaging contrast agent with the brown algal polysaccharide serving as the carrier. The macromolecular contrast agent high in water solubility is prepared by taking the brown algal polysaccharide as the carrier, taking mannose or mannose derivatives as a mannose receptor MBP (mannose binding protein) recognition group and taking paramagnetic metal ion chelates as a nuclear magnetic resonance imaging group. Lymph tissue binding force is increased, and combination of the mannose or mannose derivative group introduced into the synthetic contrast agent with mannose receptors enriched in lymph tissues is realized. In addition, after hypodermic injection of the contrast agent, lymph vessels and lymph glands are clearly developed under scanning of a nuclear magnetic resonance equipment, lymph gland signal enhancement rate of one side, with the contrast agent injected, of an animal body is remarkably increased while enhancement time is remarkably prolonged, distinct drawing and precise positioning of the lymph glands and the lymph vessels are realized, and a great significance to examination and diagnosis of lymphatic diseases is achieved.

The present invention relates to an improved endoscopic tissue apposition device having multiple suction ports. The invention permits multiple folds of tissue to be captured in the suction ports with a single positioning of the device and attached together by a tissue securement mechanism such as a suture, staple or other form of tissue bonding. The improvement reduces the number of intubations required during an endoscopic procedure to suture tissue or join areas of tissue together. The suction ports may be arranged in a variety of configurations on the apposition device to best suit the desired resulting tissue orientation. The inventive tissue apposition device may also incorporate tissue abrasion means to activate the healing process on surfaces of tissue areas that are to be joined by operation of the device to promote a more secure attachment by permanent tissue bonding.

The present invention relates to a nucleic acid aptamer which can specifically recognize and bind to pancreatic cancer cells or tissues. The nucleic acid aptamer can bind specifically only to pancreatic cancer cells or tissues without binding to normal pancreatic cancer tissue, and thus can be effectively used as a composition for diagnosing and treating pancreatic cancer. In addition, the nucleic acid aptamer can detect not only the terminal pancreatic cancer cell line Capan-1, but also the early pancreatic cancer cell line Panc-1, and thus can be used for early diagnosis of pancreatic cancer, thereby contributing to increasing the survival rate of pancreatic cancer patients.

The present invention is directed to reporter molecules and tags that may be used to monitor a target substance in a biological system. More particularly, the present invention relates to the use of an apo metal binding protein as a reporter molecule or tag. An apo metal binding protein may be bound to a protein or tissue or introduced into a cell. The invention also relates to methods of utilizing the apo metal binding protein for detecting a cell expressing a protein of interest, localizing a protein in a cell, and designing a therapeutic agent for treating a disease or infection.

The purpose of the present invention is to provide a light control agent and explore its application, and is expected to improve the tissue binding force and convenience of the existing biological glue material by providing a new reagent or material for biological damage or homeostasis. In one of embodiment, this invention provides an agent for repairing biological damage or homeostasis, wherein the agent comprises a natural biological macromolecule modified by the photo-responsive cross-linking group.

The present invention discloses the method of preparation and use of soft tissue membranous structures into slip resistant membranes with regularly spaced surface projections on one side and concave depressions on the other side with perforations or without perforations which enhance vascular ingrowth and tissue incorporation.

The purpose of the present invention is to provide a light control agent and explore its application, and is expected to improve the tissue binding force and convenience of the existing biological glue material by providing a new reagent or material for biological damage or homeostasis. In one of embodiment, this invention provides an agent for repairing biological damage or homeostasis, wherein the agent comprises a natural biological macromolecule modified by the photo-responsive cross-linking group.

The invention belongs to the field of biomedical materials, and discloses a method for preparing a titanium implant with a surface-controllable micro-nano composite structure biofilm, comprising the following steps: (1) designing a titanium implant model with micro-pores on the surface; (2) Using the selective laser melting (SLM) technology in the additive manufacturing method to print the titanium implant model to obtain the initial product of the titanium implant; (3) Using the plasma micro-arc oxidation (PEO) technology with hydrothermal A hydroxyapatite film layer with a micro-nano composite structure is formed in situ on the surface of the initial titanium implant by post-treatment or electrophoretic deposition, so as to obtain a finished titanium implant. The invention improves the detailed structure design of the implant and the overall process design of the preparation method, imitates the biological bone tissue structure, obtains a micro-nano composite structure biofilm layer with controllable surface morphology and composition, and can increase the number of implants and surrounding areas. Tissue binding efficiency and binding strength.

The invention provides a compound wound dressing with tissue binding performance and a preparation method of the compound wound dressing. Bioactive glass / albumin composite hydrogel at least comprisesbioactive glass, albumin, polyethylene glycol containing an electrophilic functional group and buffer solution, pH (potential of hydrogen) of the buffer solution ranges from 5.0 to 8.0, and the raw materials are mixed into gel to obtain the composite hydrogel. The composite hydrogel comprises albumin hydrogel and the bioactive glass loaded into the albumin hydrogel, the content of the albumin hydrogel is 95% or more, and the content of the bioactive glass is 5% or less.

The invention provides a bacterial-cellulose-based asymmetric bidirectional isomerism biological mesh and a preparation method thereof. The preparation method includes the steps that a smooth semi-drymembrane of bacterial cellulose is obtained by slow dehydration and drying, then micropores are formed in the bacterial cellulose side, then the side with the micropores is closely attached to the mold-controlled modified surface, and finally, through hydrolytic condensation deposition of a long carbon chain hydrophobic modifier on the other side under high temperature and high pressure, the biological mesh with good adhesion resistance on one side and good tissue bonding and tissue repair ability on the other side is obtained. According to the bacterial-cellulose-based asymmetric bidirectional isomerism biological mesh and the preparation method thereof, on the premise of ensuring excellent comprehensive mechanical properties and biocompatibility of the bacterial cellulose, anti-adhesionproperties are improved, the preparation method is simple, the preparation cost is relatively low, the sample shape and size are easy to control, and the bacterial-cellulose-based asymmetric bidirectional isomerism biological mesh is expected to be widely used in clinical practice; and in addition, the bacterial-cellulose-based asymmetric bidirectional isomerism biological mesh can be used for repairing hernia, dura mater, tendon, myocardium, pelvic floor and other soft tissues, and can be further used for artificial blood vessels and artificial skin and other biomedical fields.

The invention discloses HGG (Human Gammaglobulin) polypeptide in combination with the tissue specificity of cerebral arterial thrombosis. The invention relates to a method for obtaining the polypeptide. The method comprises the following steps: carrying out in-vivo selection of a mouse MCAO (Middle Cerebral Artery Occlusion) cerebral arterial thrombosis model by adopting an in-vivo phage display peptide library screening technology so as to obtain phage clones in combination with cerebral arterial thrombosis tissue; and randomly selecting the plurality of phage clones to sequence, and authenticating the in-vivo combination specificity of HGG peptide and coded phage clones HGG-M13 thereof. The invention further relates to application of the polypeptide in preparation of a high-sensitivity imaging molecular probe and a targeting delivery neuroprotective drug for cerebral stroke. The polypeptide can be synthesized through an artificial method; the polypeptide is low in molecular weight, high in activity and penetrating power, good in specificity and low in toxicity, and has good tissue targeting of cerebral arterial thrombosis in vivo; and therefore, the polypeptide is applicable to serving as a carrier of the high-sensitivity imaging molecular probe and the targeting delivery neuroprotective drug.

The invention discloses a preparation method of adhesive sodium hyaluronate gel for injection. The preparation method comprises the following steps: synthesizing a cross-linking agent A: synthesizing the cross-linking agent A containing disulfide bonds; cross-linking: reacting sodium hyaluronate with a cross-linking agent A and a cross-linking agent B to obtain gel L; preparing gel M: treating the gel L by using glutathione to obtain the gel M; treating the gel M: dialyzing the gel M to obtain the adhesive sodium hyaluronate gel for injection. The invention also discloses the adhesive sodium hyaluronate gel for injection prepared by the preparation method. The invention further discloses adhesive sodium hyaluronate gel for injection, the adhesive sodium hyaluronate gel comprises the compound with the structure shown in the formula (I), and n is 744-1985. The adhesive sodium hyaluronate gel for injection provided by the invention has good mechanical properties, is easy to inject, has strong binding force with tissues, does not generate obvious displacement in the tissues, and has good biocompatibility. The sodium hyaluronate gel disclosed by the invention is simple in preparation process, short in dialysis time, low in energy consumption and free of by-products.

The invention is an improved packaging assembly for storing, distributing, treating, mixing, and dispensing tissue and / or cellular material and / or implantable material. The packaging assembly may include pouches, tubes, and a bag made of a sealable, flexible polymeric material that is open at one end and a needle-free swabable connector attached to the pouch at the other end and acting as a port to allow for the introduction or discharge of biological solutions, rinsing solution, and / or preservation solutions into the packaging assembly. The designed thickness of the wall of the packaging assembly facilitates efficient heat / cold transfer, which is useful for successful controlled rate freezing, quick thawing, and resuscitation of viable cells or tissue. The invention is also useful for combining additional biological fluids with the cellular material or tissue, and for efficient mixing of the biological fluids with the tissue and / or cellular material in the assembly.

The invention discloses a method for automatic liver segmentation of abdominal CT sequence images based on level set and shape descriptor, which includes: preprocessing the input image to remove irrelevant organs and tissues; The correlation between adjacent slices in the sequence was used to construct a level set energy function. Starting from the initial slice, an iterative strategy was used to complete the automatic liver segmentation of abdominal CT sequence images; local and global shape descriptors were constructed to remove over-segmented regions and optimize the liver edge. The method of the present invention can effectively segment the liver area in the abdominal CT sequence image which is seriously polluted by noise and has gray scale heterogeneity, can effectively avoid wrong segmentation of adjacent tissues around the liver, remove over-segmented areas caused by gray scale overlap, and improve Liver segmentation accuracy.