53 results about "Acidic Region" patented technology

Fibroblast growth factor receptor (FGFR) extracellular domain (ECD) acidic region muteins that have been engineered to exhibit decreased tissue binding by increasing the number of acidic amino acid residues within the D1-D2 linker region are provided. Polynucleotides encoding FGFR ECD acidic region muteins are also provided. Methods of making FGFR ECD acidic region muteins, and methods of using such molecules to treat proliferative disorders, including cancers, disorders of angiogenesis, and macular degeneration, are also provided.

Fibroblast growth factor receptor (FGFR) extracellular domain (ECD) acidic region muteins that have been engineered to exhibit decreased tissue binding by increasing the number of acidic amino acid residues within the D1-D2 linker region are provided. Polynucleotides encoding FGFR ECD acidic region muteins are also provided. Methods of making FGFR ECD acidic region muteins, and methods of using such molecules to treat proliferative disorders, including cancers, disorders of angiogenesis, and macular degeneration, are also provided.

Novel microgels, microparticles and related polymeric materials capable of delivering bioactive materials to cells for use as vaccines or therapeutic agents. The materials are made using a crosslinker molecule that contains a linkage cleavable under mild acidic conditions. The crosslinker molecule is exemplified by a bisacryloyl acetal crosslinker. The new materials have the common characteristic of being able to degrade by acid hydrolysis under conditions commonly found within the endosomal or lysosomal compartments of cells thereby releasing their payload within the cell. The materials can also be used for the delivery of therapeutics to the acidic regions of tumors and sites of inflammation.

It is intended to provide a soybean protein material which is excellent in solubility, stability, emulsifying properties and gel-forming properties under acidic conditions and thus advantageously usable in acidic foods, its production process, and acidic foods using the soybean protein material. A solution containing soybean protein is subjected to a treatment for eliminating or inactivating polyanionic substances contained therein and / or adding a polycationic substance and then heated at above 100° C. under acidic conditions. Thus, a soybean protein having a high solubility under acidic conditions and thus being appropriately usable in acidic foods can be obtained. By using this protein, protein foods in an acidic region can be provided. Further, by combining the above-described treatment with a protease-digestion treatment, a soybean protein hydrolysate having a high solubility in an acidic region can be efficiently obtained.

Aqueous Li / Air secondary battery cells are configurable to achieve high energy density and prolonged cycle life. The cells include a protected a lithium metal or alloy anode and an aqueous catholyte in a cathode compartment. The aqueous catholyte comprises an evaporative-loss resistant and / or polyprotic active compound or active agent that partakes in the discharge reaction and effectuates cathode capacity for discharge in the acidic region. This leads to improved performance including one or more of increased specific energy, improved stability on open circuit, and prolonged cycle life, as well as various methods, including a method of operating an aqueous Li / Air cell to simultaneously achieve improved energy density and prolonged cycle life.

A separator for a lead-acid battery, a pasting paper for a lead-acid battery, a plate for a lead-acid battery, and a lead-acid battery using the same are disclosed. The separator comprises a microporous sheet containing a heavy metal adsorbent comprising a rare earth compound having high heavy metal adsorbability in a neutral region and low heavy metal adsorbability in an acidic region, wherein the heavy metal adsorbent is unevenly distributed in a thickness direction of the sheet, thereby forming the heavy metal adsorbent-containing layer extending in a horizontal direction of the sheet and a layer which does not substantially contain the heavy metal adsorbent.

A hair dye composition comprising a component (A), which is a specified long-chain acyl sulfonic acid salt anionic surfactant, in an amount of 0.1 to 10.0 wt. %; a component (B), which is an aliphatic alcohol, in an amount of 0.25 to 25.0 wt. %; and a component (C), which is an acid dye and / or a natural dyestuff. The hair dye composition is stable even in a strongly acidic region (pH 1.5 to 4.5) where it exerts a dyeing effect. It has an appropriate viscosity and provides excellent sensation upon use. Furthermore, the composition can be easily rinsed off after use thereof.

A modified protein of an extracellular domain of protein A, which has the reduced ability to bind to immunoglobulin in an acidic region, compared with the wild-type extracellular domain of protein A, without impairing a selective antibody-binding activity in a neutral region. On the basis of three-dimensional structure coordinate data on a complex of the extracellular domain of protein A bound with the Fc region of immunoglobulin G, the modified protein is obtained by the substitution of amino acid residues that are located within the range of 10 angstroms from the Fc region and have a 20% or more ratio of exposed surface area, by histidine residues. Preferably, the modified protein is obtained by the substitution of amino acid residues at sites identified from the analysis of sequences selected from a library constituted by the protein group, by histidine residues. These substitutions may be combined.

The present invention provides modified zirconia fine particles which are stable in an acidic region as well as in an alkaline region, and which may be readily adjusted in refractive index in a predetermined range. The present invention further provides a substrate with a hard coat film excellent in adhesiveness with the substrate, abrasion resistance, scratch strength, pencil hardness and the like without interference fringes and a coating solution which may form the hard coat film.The substrate with a hard coat film is composed of composite oxide particles formed on at least one surface of the substrate and a matrix component, wherein the composite oxide particles are composite oxide particles having a core-shell structure composed of a core formed from zirconium oxide and a shell formed from antimony pentoxide and / or silica.

Novel main chain acid degradable polymer backbones and drug delivery systems comprised of materials capable of delivering bioactive materials to cells for use as vaccines or other therapeutic agents are described. The polymers are synthesized using monomers that contain acid-degradable linkages cleavable under mild acidic conditions. The main chain of the resulting polymers readily degrade into many small molecules at low pH, but remain relatively stable and intact at physiological pH. The new materials have the common characteristic of being able to degrade by acid hydrolysis under conditions commonly found within the endosomal or lysosomal compartments of cells thereby releasing their payload within the cell. The materials can also be used for the delivery of therapeutics to the acidic regions of tumors and other sites of inflammation.

Novel microgels, microparticles and related polymeric materials capable of delivering bioactive materials to cells for use as vaccines or therapeutic agents. The materials are made using a crosslinker molecule that contains a linkage cleavable under mild acidic conditions. The crosslinker molecule is exemplified by a bisacryloyl acetal crosslinker. The new materials have the common characteristic of being able to degrade by acid hydrolysis under conditions commonly found within the endosomal or lysosomal compartments of cells thereby releasing their payload within the cell. The materials can also be used for the delivery of therapeutics to the acidic regions of tumors and sites of inflammation.

The invention discloses a fluorescent probe for identifying hydrogen sulfide in lysosome. The fluorescent probe is 2-(9-(2-ethoxy ethyl ester)-2-(pyridyl-4-yl)vinyl)-9H-carbazolyl-3-yl)vinyl)-1-ethyl-3,3-dimethyl-3H-indolyl-1-iodine which is called PCAI for short, and the chemical structural formula is disclosed as Formula (I). The invention also discloses application of the fluorescent probe in detecting H2S in lysosome. The experiment proves that the fluorescent probe can identify lysosome and H2S in the lysosome through fluorescence enhancement; and the probe has potential application value in the field of targeted molecular markers of acidic regions of living cells.

A modified protein of an extracellular domain of protein A, which has the reduced ability to bind to immunoglobulin in an acidic region, compared with the wild-type extracellular domain of protein A, without impairing a selective antibody-binding activity in a neutral region. On the basis of three-dimensional structure coordinate data on a complex of the extracellular domain of protein A bound with the Fc region of immunoglobulin G, the modified protein is obtained by the substitution of amino acid residues that are located within the range of 10 angstroms from the Fc region and have a 20% or more ratio of exposed surface area, by histidine residues. Preferably, the modified protein is obtained by the substitution of amino acid residues at sites identified from the analysis of sequences selected from a library constituted by the protein group, by histidine residues. These substitutions may be combined.

The invention relates to the deep processing field of soy bean protein. Specifically, the present invention relates to an acid soluble soy protein preparation method which includes the following steps: alkali dissolving, acid precipitating and centrifugating a defatted soy bean residue to obtain soybean protein isolate precipitation which is dissolved in water to form a slurry, adjusting the pH of the slurry to 1.0 to 4.0 using an acidity regulator, stirring and completely dissolving the soybean protein isolate precipitation to obtain a protein liquid a; microwave digesting the protein liquid a and separating the protein liquid a through centrifugation; treating supernatants obtained by centrifugation at a temperature of 100-180 DEG C and subsequently cooling the treated supernatants; and at last, obtaining the acid soluble soy protein through spray drying. The acid soluble soy protein preparation method has the following advantages of high production efficiency, simple process, low production cost, and high product safety; and produced acid soluble soy protein has a relatively high solubility in the acidic region, and thus acidic beverage containing the acid soluble soy protein has less precipitation, a good taste and a high nutritional value.

This invention provides an economical slime-controlling and deodorizing method in a papermaking process. The method for sterilizing and deodorizing water for the papermaking comprises adding a high-concentration aqueous solution of sodium hypochlorite in a hydrochloric acid condition, having pH>=4 and 1,000-10,000 ppm, preferably 2,000-8,000 ppm effective chlorine concentration to a water tank for a white water system or a recovered scum system, and regulating the effective chlorine concentration of the water so as to be 100-10 ppm. The method enables the reduction of the papermaking slime or deodorization to be achieved at an economical cost. The water for the papermaking after addition is effective even in a neutral region but is preferably used in a weak acidic region.

Provided is a liquid food composition capable of semi-solidifying in the stomach, which is a one-pack type product containing a water-soluble dietary fiber preliminarily added thereto and in the form of a liquid that can be easily taken, and stably sustains the liquid nature thereof during distribution and storage. The liquid food composition, which is capable of semi-solidifying in an acidic region, comprises a water-soluble dietary fiber (a), a specific metal compound (b), a protein (c) and an emulsifier (d), and the particle size distribution of particles contained in said liquid food composition shows two or more peaks in a neutral region.

Novel main chain acid degradable polymer backbones and drug delivery systems comprised of materials capable of delivering bioactive materials to cells for use as vaccines or other therapeutic agents are described. The polymers are synthesized using monomers that contain acid-degradable linkages cleavable under mild acidic conditions. The main chain of the resulting polymers readily degrade into many small molecules at low pH, but remain relatively stable and intact at physiological pH. The new materials have the common characteristic of being able to degrade by acid hydrolysis under conditions commonly found within the endosomal or lysosomal compartments of cells thereby releasing their payload within the cell. The materials can also be used for the delivery of therapeutics to the acidic regions of tumors and other sites of inflammation.

To produce a bifunctional adsorbent capable of adsorption in the acidic region, a polymeric substrate is first exposed to a radiation and then a phosphonic acid group suitable for metal adsorption and a sulfonic acid group are introduced into the substrate by graft polymerization.

It is intended to provide a silage additive which improves silage fermentation and enriches livestock feeds at a low cost. Namely, a silage additive comprising a liquid by-product of amino acid fermentation having a pH value adjusted in an acidic region (3.0 to 6.0).

Disclosed is a technique for sustaining an excellent suitability as a liquid food in a thick liquid food which is rich in protein and minerals (in particular, compounds of the group II elements), whereby stable characteristics without any aggregation in a heat sterilization step or with the passage of time can be imparted. It is found out that the aggregation in a thick liquid food caused by reactions between minerals and protein in a heat sterilization step or with the passage of time can be prevented as far as possible by selecting a soybean protein material, which has a high crude protein content and a relatively low solubility in water and shows a pH value in the slightly acidic region when dissolved in water, together with a dispersing agent.

When industrially separating and purifying poly-3-hydroxyalkanoic acid produced by a microorganism, to obtain poly-3-hydroxyalkanoic acid agglomerates having an arbitrary volume mean particle diameter with favorable productivity and with decreased amount of an organic solvent used is enabled while decreasing contaminants derived from constitutive components of cellular bodies. According to the present invention, agglomerates of poly-3-hydroxyalkanoic acid are obtained by adjusting the pH of an aqueous poly-3-hydroxyalkanoic acid suspension to fall within an acidic region.

Provided is a cultured milk manufacturing method with which taste and quality can be kept constant regardless of the lactic acid bacteria or enzyme conditions. The cultured milk manufacturing method comprises a fermentation process to ferment raw milk that contains an enzyme, and a deoxygenation process that is carried out prior to the fermentation. The enzyme exhibits optimal pH for activation in the neutral region and is inactivated in the acidic region, and the enzyme can break down lactose contained in the raw milk while in the active state. As demonstrated with working examples, by carrying out the deoxygenation process prior to the fermentation, the taste and quality of the cultured milk can be kept constant regardless of the lactic acid bacteria or enzyme conditions.

It has been demanded to provide a method for producing a granulated matter capable of maintaining a stability of a chemically unstable substance in a neutral or acidic region for a long period of time with a simple and safe method in a preparation procedure, and a tablet produced by using the method. The invention provides a granulation method in which an unstable substance is successively subjected to aqueous stabilization treatment and granulation procedure. Further, it became possible to provide a tablet which is absorbed in the intestine without losing the potency in a gastric region by forming an intermediate layer on a surface of the thus obtained granule and subsequently subjecting the granule to enteric coating.

Modified zirconia fine particles which are stable in an acidic region as well as in an alkaline region, and which may be readily adjusted in refractive index in a predetermined range are disclosed. Also disclosed is a substrate with a hard coat film excellent in adhesiveness with the substrate, abrasion resistance, scratch strength, pencil hardness and the like without interference fringes and a coating solution which may form the hard coat film. The substrate with a hard coat film is composed of composite oxide particles formed on at least one surface of the substrate and a matrix component, wherein the composite oxide particles are composite oxide particles having a core-shell structure composed of a core formed from zirconium oxide and a shell formed from antimony pentoxide and / or silica.

This invention relates to a method for measuring autophagy in cells, comprising using, as a probe reagent, a single fluorescent protein, to measure a change in fluorescence properties of the fluorescent probe reagent depending on pH changes associated with autophagy, thereby determining the presence or activity of autophagy, wherein the single fluorescent protein is resistant to degrading enzyme activity in the lysosome or vacuole of the cell, it is not denatured or inactivated under acidic to neutral pH environment, and it is capable of changing excitation spectra or fluorescence spectra when located under the environments of acidic region and neutral region.

The present invention relates to a CMP slurry composition, for polishing an organic film, comprising: cerium salts comprising ultrapure water, abrasives and cerium (IV) ions; and an oxidizing agent, wherein the oxidizing agent has an oxidation potential of 1.72eV or higher in an acidic region and has a pH value of from 1 to 7. The CMP slurry composition enables stabilization of cerium (IV) ions (Ce4+) even at a pH of 1 or higher. Therefore, the present invention shows enhanced polishing effects on an organic film and enables easily organic film polishing, and particularly self-aligned double patterning, by means of an organic film polishing method using the CMP slurry composition.

Provided is a liquid food composition capable of semi-solidifying in the stomach, which is a one-pack type product containing a water-soluble dietary fiber preliminarily added thereto and in the form of a liquid that can be easily taken, and stably sustains the liquid nature thereof during distribution and storage. The liquid food composition, which is capable of semi-solidifying in an acidic region, comprises a water-soluble dietary fiber (a), a specific metal compound (b), a protein (c) and an emulsifier (d), and the particle size distribution of particles contained in said liquid food composition shows two or more peaks in a neutral region.

A compound represented by the following general formula (I):wherein R1 represents an amino group which may be substituted with one or two alkyl groups; R2, R3, R4 and R5 independently represent an alkyl group; and R6 and R7 independently represent a monocarboxyalkyl group, a salt thereof, or an ester thereof, which is useful as a pH-sensitive fluorescent probe which emits intense fluorescence in an acidic region.

The purpose of the present invention is: to provide an excellent protein which is further reduced in the binding property to an Fc region of an immunoglobulin and / or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a protein containing an extracellular domain of wild-type protein G, and which still keeps a high antibody-binding activity in a neutral region; and to capture and collect an antibody readily using the protein without denaturating the antibody. The present invention relates to: a protein that is reduced in the binding property to an Fc region of an immunoglobulin and / or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a multimer comprising an extracellular domain of wild type one, which is a domain having a binding activity to a protein comprising an Fc region of immunoglobulin G, while keeping a high antibody-binding activity in a neutral region, and also has a binding activity to a protein comprising the Fc region of immunoglobulin G, wherein the protein comprises a tandem-type multimer of a mutant of the extracellular domain; and others.

The present invention provides a composition for measuring residual chlorine concentration in the sample water, which is an aqueous solution comprising a specific benzidine compound, e.g., N,N′-bis(2-sulfoethyl)-3,3′-dimethylbenzidine disodium salt, and a surfactant, wherein the content of the surfactant is set to be 0.5% by weight or more and to be 0.5 weight-fold or more of the content of the benzidine compound, and the pH is adjusted to be in the acidic region.This composition can prevent the benzidine compound from crystallizing in the aqueous solution under the low temperature condition such as in winter season or the like.