Acid soluble soy protein preparation method
A technology for soybean protein and soybean protein isolate, applied in the field of preparing acid-soluble soybean protein, can solve the problems of high cost, short reaction time, production cost, long reaction time, etc. Effect
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Embodiment 1
[0069] 10kg of defatted soybean meal was dissolved in 100kg of water, and 2mol / L sodium hydroxide solution was added dropwise therein to adjust the pH to 7.0, stirred evenly, and centrifuged at 2000G for 30min. The supernatant after centrifugation was taken out, and 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 3.0, followed by centrifugation at 3000G for 15 minutes to obtain a soybean protein isolate precipitate. The soybean protein isolate precipitate prepared above was dissolved in water to form a 5wt% slurry, and 1mol / L hydrochloric acid solution was added dropwise to the slurry to adjust the pH to 2.0, and the soybean protein isolate precipitate was completely dissolved by stirring to obtain protein liquid.
[0070] The above protein solution was subjected to microwave digestion treatment, and the microwave digestion treatment used the ETHOS T microwave digestion / extraction system of Italy Milestone Company, and the specific ...
Embodiment 2
[0072] Dissolve 10kg of soybean defatted soybean meal in 150kg of water, and dropwise add 2mol / L sodium hydroxide solution to adjust the pH to 8.0, stir evenly, and centrifuge at 3000G for 15min. The centrifuged supernatant was taken out, and 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 4.5, followed by centrifugation under 4000G centrifugal force for 15 minutes to obtain soybean protein isolate precipitate. The soybean protein isolate precipitate prepared above was dissolved in deionized water to form a 7wt% slurry, 2mol / L hydrochloric acid solution was added dropwise to the slurry to adjust the pH to 2.5, and the soybean protein isolate precipitate was completely dissolved by stirring, get protein.
[0073] The above protein solution was subjected to microwave digestion treatment, and the microwave digestion treatment used the ETHOS T microwave digestion / extraction system of Italy Milestone Company, and the specific parameters ...
Embodiment 3
[0075] Dissolve 10kg of soybean defatted soybean meal in 200kg of water, add dropwise 2mol / L sodium hydroxide solution to adjust the pH to 9.0, stir well and centrifuge at 4000G for 10min. The centrifuged supernatant was taken out, and 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 6.0, followed by centrifugation under 2000G centrifugal force for 30 min to obtain soybean protein isolate precipitate. The soybean protein isolate precipitate prepared above was dissolved in deionized water to form a 9wt% slurry, and a 2mol / L phosphoric acid solution was added dropwise to the slurry to adjust the pH to 3.5, and the soybean protein isolate precipitate was completely dissolved by stirring, get protein.
[0076] The above protein solution was subjected to microwave digestion treatment, and the microwave digestion treatment used the ETHOS T microwave digestion / extraction system of Italy Milestone Company, and the specific parameters were: 8...
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