39 results about "Lyophyllum decastes" patented technology

The invention relates to a lyophyllum decastes cultivation method. The lyophyllum decastess cultivation method comprises the following steps: weighing culture medium raw materials in parts by mass; mixing the raw materials; controlling the pH value to be 6-7 and putting the materials into culture bottles, wherein 400-500 parts of the raw materials are put into each bottle; pressing a cap and sealing; conveying the culture bottle into a vacuum sterilization pot; sterilizing with ozone for 1 to hours until the vacuumizing degree is 50KPa-80KPa; sterilizing at 150 DEG C for 100-120 minutes; cooling at 15-20 DEG C and adding 1-4 parts of humic acid; cooling and conveying a mixture into each bottle to be inoculated for 40-50 minutes; culturing in a culture room at 20-25 DEG C for 20-25 days under the conditions that the humidity is 70%-80% and the concentration of carbon dioxide is 1600PPM-3200PPM; carrying out mycelium stimulation: carrying out mycelium stimulation treatment by using a mycelium stimulation machine and supplying water according to 85% of the weight of old mycoderma; carrying out bud promotion and collecting: after the mycelium stimulation, starting to collect after 21 days in the culture room under the conditions that the temperature is 14-15 DEG C, the humidity is 95%-98%, the concentration of the carbon dioxide is under 1200PPM, the illumination intensity of 0-50Lux from day 1 to day 3, the illumination intensity of 50Lux-100Lux from day 4 to day 6, the illumination intensity of 150Lux-200Lux from day 7 to day10 days, and the illumination intensity of 200Lux-300Lux for day 10 to day 20.

The invention relates to the technical field of edible fungus, particularly to a generating method of original seeds of Lyophyllum decastes, including the following steps: composting process of saw dust, preparation of stock culture ground substrance, bottling, sterilization, inoculation and culture. The inventive method causes the hairy fungus period of the original seed of the Lyophyllum decastes to shorten from 35-38 days to 30-32 days, hypha colonization ability after inoculation is shortened from 10-15 days to 7-10 days, cultivation bottle-filling time is shortened by 5-7 days.

The invention relates to a molecular marker for identifying lyophyllum decastes, and a primer and a probe. An ITS specificity molecular marker of the lyophyllum decastes has a nucleotide sequence shown in SEQ ID NO.1. A gene chip for specificity detection of the lyophyllum decastes is designed for the molecular marker, the lyophyllum decastes can be subjected to specificity identification within a short time, the identification accuracy and sensitivity of the lyophyllum decastes are improved, and the molecular marker has the characteristics of quickness, accuracy, and low cost.

The invention relates to a preparation and regeneration method of a lyophyllum decastes protoplast. The preparation and regeneration method comprises the following steps of treating lyophyllum decastes hyphae having fungus age of 72-96h by a cane sugar osmotic pressure stabilizing agent having content of 0.6mol / L, carrying out enzymolysis on a mixed enzyme liquid containing 1% by mass of lywallzyme and 0.5% by mass helicase at a temperature of 30 DEG C for 2.5-3h, preparing a self-made needle filter, and carrying out separation by the self-made needle filter to obtain the lyophyllum decastes protoplast. The preparation and regeneration method has a protoplast yield of 4.79*10<7> per milliliter (5.76*10<5> per milligram) and a protoplast regeneration rate of 7.81%. Compared with single lywallzyme, the mixed enzyme liquid containing lywallzyme and helicase improves protoplast preparation and regeneration rate and reduces a cost. The preparation and regeneration method utilizes a small-needle and small-area filtration method so that a cost is lower than that of the routine method. The preparation and regeneration method lays a foundation for lyophyllum decastes breeding adopting the protoplast technology.

The invention belongs to the technical field of edible fungi and particularly relates to a lyophyllum decastes strain Finc-L6 and a cultivation method and application thereof. The lyophyllum decastes strain is named Finc-L6, and obtained in the mode that a strain Finc-LD-2 and a strain Finc-LD-3 serve as parents to carry out hybridization, and systemic breeding is carried out, the lyophyllum decastes strain belongs to Lyophyllum decastes and is preserved in the common microorganism center of the China microorganism strain preservation management council, the address of the center is room 3, Beichen west road No.3, Chaoyang district of Beijing, China, the preservation serial number is CGMCC No.11201, and the preservation date is August 13th, 2015. According to the technical scheme, the blank of lyophyllum decastes hybridization breeding is filled up, and the obtained novel strain is short in cultivation period, high in per unit yield, beautiful in flower shape and suitable for large-area plantation and popularization, fruiting time is centralized, the freshness keeping period is long, and the market prospect is wide.

The invention pertains to the technical field of edible fungus, in particular to a lyophyllum decastes Finc-L10 strain, a cultivation method and application thereof. The name of the lyophyllum decastes strain is Finc-L10 and the lyophyllum decastes strain is obtained by system selection in such a manner that a strain Finc-LD-2 and a strain Finc-LD-3, as parents, are subjected to hybridization, pertaining to lyophyllum decastes and preserved in the institute of microbiology, the address of which is BeiChen West road 1st courtyard NO.3 in Chaoyang District of Beijing. The preservation number is CGMCC NO.11202. The preservation time is 2015, 08, 13. By adoption of the technical scheme, the gap for crossbreeding of lyophyllum decastes is filled. The cultivation period is short and time for producing mushrooms is concentrated. The cultivation method is high in yield per unit, attractive in flower shapes, few in deformed mushrooms, uniform in external appearance and high in effective number of mushroom buds and suitable for large-area promotional plantation. A broad market prospect is obtained.

The invention relates to a main material of an edible fungus culture medium, in particular to the main material of the culture medium of lyophyllum decastes. The main material of the culture medium of the lyophyllum decastes is characterized by being obtained by composting wastes obtained after fermentation and cultivation of edible wood-rotting fungi. The main material of the culture medium of the lyophyllum decastes solves the problem of high cost caused by taking bark compost as the main material now.