Fermentation technology of selenium-enriched crude intercellular polysaccharide in lyophyllum decastes mycelium

The invention relates to a technology of lotus leaf folded umbrella and fermentation process, which is applied in the field of bioengineering and can solve the problems of low efficiency of selenium-enriched crude polysaccharide and the like.

Inactive Publication Date: 2014-11-19
GANSU QILIAN EDIBLE FUNGI ENG TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problem of low efficiency in the production of selenium-enriched crude polysaccharides, the present invention provides a fermentation process for selenium-enriched intracellular crude polysaccharides

Method used

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  • Fermentation technology of selenium-enriched crude intercellular polysaccharide in lyophyllum decastes mycelium
  • Fermentation technology of selenium-enriched crude intercellular polysaccharide in lyophyllum decastes mycelium
  • Fermentation technology of selenium-enriched crude intercellular polysaccharide in lyophyllum decastes mycelium

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Experimental program
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Effect test

Embodiment 1

[0061] In a 250mL Erlenmeyer flask, take 100mL of the liquid medium prepared above and 1mg / mL Na 2 SeO 3 solution, mix the two and sterilize at 121°C for 20 minutes, make the concentration of selenium in the liquid medium be 10ug / mL when mixing, cool to 20~25°C, insert 6 pieces of lotus leaves with a diameter of 6mm Cultivate the activated strain block of Li phymeji at 20~25°C and 121rpm until the 12th day after inoculation, and the selenium-enriched intracellular crude polysaccharide content of the obtained Li phyme mycelia is 22.6mg / 100mL.

Embodiment 2

[0063] In a 250mL Erlenmeyer flask, take 100mL of the liquid medium prepared above and 1mg / mL Na 2 SeO 3 solution, the two were sterilized at 121°C for 20 minutes, and then mixed, so that the concentration of selenium in the liquid medium was 10ug / mL when mixed, cooled to 20~25°C, and inserted into 6 pieces of 6mm in diameter The activated strain block of the lotus leaf mushroom was cultivated at 20~25°C and 121rpm until the 12th day after inoculation, and the selenium-enriched intracellular crude polysaccharide content of the obtained lotus leaf mushroom mycelium was 70.8mg / 100mL.

Embodiment 3

[0065] In a 250mL Erlenmeyer flask, add liquid medium 100mL and 1mg / mL Na 2 SeO 3 Solution, take the liquid medium and Na 2 SeO 3The different methods of mixing the solutions after they are sterilized separately and after mixing them, the sterilization conditions of the two different methods are 115°C, 20min, 115°C, 30min, 121°C, 15min, 121°C, 20min , 121°C, 30min; when mixing, the concentration of selenium in the liquid medium was 10ug / mL, after cooling to 20~25°C, insert 6 pieces of activated bacterial seed blocks of the lotus leaf with a diameter of 6mm. Cultivate at 22°C and 121rpm until the 12th day after inoculation. Three parallel control experiments were set up for each sterilization condition to measure the content of crude polysaccharide in the selenium-enriched mycelia of A. pleinosaurs. The results are as follows: figure 2 Shown, liquid medium and Na 2 SeO 3 The content of selenium-enriched intracellular polysaccharides in the selenium-enriched mycelium of th...

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Abstract

The invention discloses a fermentation technology of selenium-enriched crude intercellular polysaccharide in lyophyllum decastes mycelium. According to the fermentation technology, low-cost inorganic Na2SeO3 is adopted as a selenium source, a Na2SeO3 solution is added in a liquid culture medium prepared from potato glucose in a manner of liquid fermentation cultivation, Na2SeO3 is converted into organic selenium by virtue of enrichment of the lyophyllum decastes mycelium, so that the toxicity of selenium can be reduced, and the utilization value of the mycelium can be improved; Na2SeO3 is added in the intermediate fermentation phase of the liquid culture medium, so that the inhibition and toxic action of the selenium on the lag-phase lyophyllum decastes mycelium can be effectively reduced, and the accumulation amount of the selenium-enriched intercellular polysaccharide in lyophyllum decastes mycelium can be increased. By virtue of a single-factor experiment and a Box-Behnken experiment design as well as a response surface analysis method, the fermentation conditions of selenium-enriched intercellular polysaccharide in lyophyllum decastes can be optimized to obtain optimal fermentation conditions, and a quadratic regression equation model of the intercellular polysaccharide and factor variables of fermentation conditions can be obtained, and the model is extremely remarkable in regression and better in experiment fitting, thus having certain application values.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a production process of selenium polysaccharides, in particular to a fermentation process of selenium-enriched intracellular crude polysaccharides of lotus leaf pleils. Background technique [0002] Lotus Leaf Shimegum ( Lyophyllum decays ) Mycelium has high protein content, a complete range of amino acids, and contains a variety of vitamins. Its polysaccharides can inhibit the growth of tumors and reduce the blood sugar concentration of insulin-dependent mice. It is a bacterium with high nutritional and medicinal value. kind. Moreover, the mycelium of the lotus leaf phymeji mushroom can effectively enrich a certain amount of selenium. Edible fungi have a strong ability to enrich selenium. Through the metabolism of substances in mycelium cells, inorganic selenium can be safely and effectively converted into organic selenium polysaccharides. Its biopharmacological activity is general...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/645
Inventor 高慧娟魏生龙席亚丽梁倩倩刘志芳袁承玲赵丽
Owner GANSU QILIAN EDIBLE FUNGI ENG TECH RES CENT
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