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Lyophyllum decastes strain, cultivation method and application thereof

A technology of lotus-leaf-shimeji mushroom and its cultivation method, which is applied to the lotus-leaf-shimeji mushroom strain and its cultivation method and its cultivation method and application field, and can solve the problems of long cultivation period of the strain, low rate of good products, and inability to meet industrialized cultivation Conditions and market demand and other issues, to achieve the effects of short cultivation period, high yield per unit area, and favorable mechanization and automation operations

Active Publication Date: 2016-01-13
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a lotus leaf phyllomyces strain and its cultivation method and its application, which are used to solve the problems of long cultivation cycle and low yield rate of the current strains, which cannot meet the conditions of industrialized cultivation and market demand.

Method used

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  • Lyophyllum decastes strain, cultivation method and application thereof
  • Lyophyllum decastes strain, cultivation method and application thereof
  • Lyophyllum decastes strain, cultivation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 hybridization obtains Finc-L10

[0049] Finc-L10 was obtained by hybrid breeding method.

[0050] Specific steps are as follows:

[0051] 1) Select parental strains: select the strains Finc-LD-2 and Finc-LD-3 domesticated by Shanghai Fengke Biotechnology Co., Ltd. as parents;

[0052] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;

[0053] 3) Isolation of basidiospores: place the caps of Finc-LD-2 and Finc-LD-3 in a petri dish respectively, after 24 hours, collect the spores falling on the petri dish, and dilute them into an appropriate amount in sterile water. Concentration, and spread on the PDA medium plate. Cultivate at 23°C for 25 days, and inoculate PDA slant test tubes with inoculation loops after the spores germinate. These tubes were incubated at 23°C for 25 days, after which hyphae were isolated from the tubes...

Embodiment 2

[0057] Embodiment 2 Antagonism experiment

[0058] Finc-L10 of the present invention, 2 parent strains Finc-LD-2, Finc-LD-3 and 2 control strains Finc-LD-3, Finc-LD-4 are test materials, and the medium used is PDA medium.

[0059] Finc-L10, Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 were inoculated and cultured on the plate medium respectively. The distance between the inoculation points was 2.5 cm, and cultured at 23°C for 25 days , see Table 1 for the test results.

[0060] Table 1 Antagonism test among different strains

[0061] Variety Finc-LD-1 Finc-LD-2 Finc-LD-3 Finc-LD-4 Finc-L10 antagonistic antagonistic antagonistic antagonistic

[0062] Such as Figure 1-4 As shown, there are obvious antagonistic lines between Finc-L10 and Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 at the junction of confrontation culture colonies, indicating that Finc-L10 is related to Finc-LD-1, Finc-LD-2, Finc-LD-3, and Finc-LD-4 are different strain...

Embodiment 3

[0063] Embodiment 3 cultivation experiment

[0064] Lotus leaves of the present invention strain Finc-L10, parent bacterial strains Finc-LD-2 and Finc-LD-3, and existing varieties Finc-LD-1, Finc-LD-4 are cultivated and cultivated respectively, each Cultivate 500 bottles, then summarize and compare the results of small batch cultivation, and summarize the advantages and characteristics of the bacterial strain Finc-L10 of the present invention.

[0065] The culture medium of this embodiment participates in the cultivar culture medium, and will not be repeated here.

[0066] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.

[0067] Cultivation: temperature 23~28℃, air relative humidity 75%, carbon dioxide concentration 2000~3500ppm;

[0068] Cultivation: The fruiting temperature is set at 15°C-18°C, the relative air humidity is 93%-100%, the carbon dioxide concentration is 1000-2000ppm, the light intensity is 10-50Lux for the first 6 days, and the light intensity ...

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Abstract

The invention pertains to the technical field of edible fungus, in particular to a lyophyllum decastes Finc-L10 strain, a cultivation method and application thereof. The name of the lyophyllum decastes strain is Finc-L10 and the lyophyllum decastes strain is obtained by system selection in such a manner that a strain Finc-LD-2 and a strain Finc-LD-3, as parents, are subjected to hybridization, pertaining to lyophyllum decastes and preserved in the institute of microbiology, the address of which is BeiChen West road 1st courtyard NO.3 in Chaoyang District of Beijing. The preservation number is CGMCC NO.11202. The preservation time is 2015, 08, 13. By adoption of the technical scheme, the gap for crossbreeding of lyophyllum decastes is filled. The cultivation period is short and time for producing mushrooms is concentrated. The cultivation method is high in yield per unit, attractive in flower shapes, few in deformed mushrooms, uniform in external appearance and high in effective number of mushroom buds and suitable for large-area promotional plantation. A broad market prospect is obtained.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a lotus-leaved mushroom strain, a cultivation method thereof, a cultivation method and an application thereof. Background technique [0002] Lotus leaf Liphyllum decastes (Latin name Lyophyllum decastes), which belongs to Basidiomycotina, Phyllomycetes, Agaricaceae, Liphyllumaceae, Liphyllum genus, is an excellent edible fungus that is being researched and cultivated in the field of edible fungi at home and abroad. The flesh of lotus leaf phyllomycetes is thick and delicate, fragrant and tangy, crisp and smooth, delicious, rich in nutrition, and has shown great advantages in the repair of human cells and in the treatment of cardiovascular diseases and anti-aging. It is a rare edible fungus that can be used both as food and medicine. Its food and medicine value has been accepted and recognized by more and more people, and it will have a broad market prospect in th...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 韩成玲吴慧敏张文宏
Owner SHANGHAI FINC BIO TECH INC
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