A kind of protoplast preparation and regeneration method of Lily mushroom
A technology of lotus leaves and protoplasts, applied in the field of bioengineering, to reduce the loss of protoplasts, reduce the cost of preparation, and improve the production rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0025] Bacteria of the present invention
[0026] The lotus-leaved mushroom strain (preservation number CGMCC1518, strain number ZY48-1, patent number ZL200510096405.0) was provided by the Edible Fungi Research Institute of Hexi University.
[0027] Reagents of the present invention
[0028] Lysozyme (Guangdong Provincial Institute of Microbiology); Helicase (Beijing Baitai Biochemical Technology Company); Cellulase (US Bettery Company); other reagents were domestic analytically pure.
[0029] Configuration of enzyme solution
[0030] Dissolve lytic enzyme and helicase with 0.6mol / L sucrose osmotic pressure stabilizer, make lytic enzyme mass concentration be 2%, helicase mass concentration be 1%, with 2% lytic enzyme and 1% helicase by volume ratio Mix 1:1, so that the concentration of lysozyme in the mixed enzyme is 1%, and the concentration of helicase is 0.5%, centrifuge at 10000r / min for 10min, take the supernatant and use 0.22 mu m microporous membrane filtration, sta...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com