Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing.

Abstract

The invention relates to a preparation method of a liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. The preparation method comprises the following steps: (1) weighing the following raw materials of the medium according to the parts by mass: 10-30 parts of sucrose, 2-5 parts of soybean meal, 1-3 parts of beef extract, 0.3-1 part of magnesium sulfate heptahydrate, 0.3-1 part of potassium dihydrogen phosphate and 0.1-0.3 part of defoaming agent; (2) mixing the raw materials in the step (1), controlling the PH value to be 5.5-6.5, putting 400-500 parts of the mixture in each culture bottle, and covering and packaging the bottles; (3) sending the culture bottles into a vacuum sterilizer, carrying out ozone sterilization for 1-2 hours, then vacuumizing until the vacuum degree is 50-80KPa, carrying out insulation at 115 DEG C for 30 minutes, and carrying out insulation at 121 DEG C for 60 minutes. The preparation method has the advantages that the medium is convenient to prepare and is low in cost; when the medium is used for preparing liquid spawns, the spawns are fast in growth, are not polluted and do not have mycoderma, thus being conducive to improving the spawn growth effects and increasing the yield.

Description

technical field [0001] The invention relates to a liquid culture medium of velvet antler mushroom, a preparation method thereof, and a preparation method of liquid strains. Background technique [0002] Antler mushroom, also known as Lyophyllum decastes (Fr. : Fr.) Sing.), lotus leaf mushroom, also known as Lengxiang fungus, a nest of sheep, Beifeng fungus, etc. in Yunnan, my country, is a basidiomycete Subphylum, Phylomycetes, Agarita, Agaricaceae, Agaricus, known as fried chicken mushroom in Europe, the meat is thick, delicate, fragrant and delicious, research shows that the crude protein, The amino acid content is high, and the fat content is low; it also contains trace elements zinc, copper and selenium that are beneficial to the human body and a large amount of vitamins B1, B2, B6, B12 and niacin, and has high nutritional value. According to modern scientific research, the β-glucan contained in pilose antler mushroom has anti-tumor effect after long-term consumption. ...

AI Technical Summary

Problems solved by technology

In 2008, Juegouxun Forestry Farm of Iturihe Forestry Bureau, Yakeshi City, Inner Mongolia Autonomous Region applied for a patent for the cultivation method of lotus-leafed mushrooms (antler mushrooms). The cultivation method of solid strains was adopted. The preparation cost of solid strains was long and the inoculation efficiency was low. , the germination speed of the bacteria is slow, and the contamination rate of the culture bottle is high