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Lyophyllum decastes polysaccharide and preparation method and application thereof

A technology for the polysaccharide of A. lily and the polysaccharide, which is applied in the field of the polysaccharide of A. lichen and its preparation

Active Publication Date: 2019-09-10
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the fine structure and immunomodulatory activity of LDS-1 polysaccharide from Xiaojin County, Sichuan Province and its application

Method used

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  • Lyophyllum decastes polysaccharide and preparation method and application thereof
  • Lyophyllum decastes polysaccharide and preparation method and application thereof
  • Lyophyllum decastes polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Separation and extraction of polysaccharide LDS-1

[0053] 1.1 Isolation and extraction of the polysaccharide LDS-1 from Phyllophyllum chrysanthemum

[0054] 1.1.1 Extraction of polysaccharides from Phyllophyta japonica by hot water extraction

[0055] Take 200 g of lotus leaf shimeji fruit body, wash, dry and pulverize it, and then extract it with distilled water at 90° C. for 3 times to obtain the crude extract of lotus leaf shimeji polysaccharide. Using the ratio of 4 parts of absolute ethanol: 1 part of crude sugar concentrate to ethanol precipitate the crude polysaccharide concentrate, collect the precipitate by centrifugation, and dry it for later use.

[0056] 1.1.2 DEAE-cellulose column chromatography separation and purification of crude polysaccharides

[0057] Weigh 40g of DEAE cellulose-52 cellulose, dissolve it in ultrapure water until there are no particles, let it settle for 6 hours, remove the ultrapure water, soak in 0.5mol / L NaOH for 6 hours...

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Abstract

The invention discloses a Lyophyllum decastes polysaccharide and a preparation method and application thereof. The polysaccharide is extracted and purified from the fruiting body of Lyophyllum decastes by water extraction and alcohol precipitation, ion exchange column chromatography and dialysis concentration. The polysaccharide is a heteropolysaccharide consisting of alpha-D-glucose and beta-D-galactose, wherein the molar ratio of residues of alpha-D-glucose to the residues of beta-D-galactose is 2: 1. The polysaccharide has significant immunomodulatory activity and inhibitory activity on various tumor cells.

Description

technical field [0001] The invention belongs to the field of application technology of fungal polysaccharides, and in particular relates to a polysaccharide from the fungal polysaccharide and its preparation method and application. Background technique [0002] Most of the fungi of the Basidiomycotina subphylum are edible and medicinal fungi. There have been records of using fungi as medicines in my country thousands of years ago, such as Ganoderma lucidum recorded in "Shen Nong's Materia Medica", and Poria cocos recorded in "Compendium of Materia Medica". The biological activities of fungal polysaccharides are complex and diverse, and they have attracted much attention because of their high medical value. A large number of experiments have proved that fungal polysaccharides can resist oxidation and aging, reduce blood pressure and lipids, nourish Qi and nourish the mind, anti-inflammatory and anti-virus, enhance the body's anti-tumor ability and improve the body's immunity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/08A61P35/00A61P37/06A23L33/125
CPCA23V2002/00A23L33/125A61P35/00A61P37/06C07H1/08C07H3/06C08B37/006A23V2200/324A23V2250/51A23V2250/208
Inventor 侯怡铃丁祥刘影
Owner CHINA WEST NORMAL UNIVERSITY
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