Sulfated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

A technology of polysaccharide sulfuric acid and hollyhock, which is applied to organic active ingredients, medical preparations containing active ingredients, food ingredients containing polysaccharides/gum, etc., can solve the problems of less active research and achieve the goal of improving immune activity and good ecological environment Protective significance, effect of significant immunomodulatory activity

Inactive Publication Date: 2018-06-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign studies on polysaccharides from stems and leaves of hollyhock mainly focus on the analysis of physical and chemical properties and monosaccharides, and there are few studies on its activity.

Method used

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  • Sulfated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Sulfated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Sulfated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of the polysaccharide sulfated modification product of the stem and leaf of hollyhock comprises the following steps:

[0031] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock

[0032] Take 100 g of dry dregs of hollyhock stems and leaves extracted by ethanol reflux, add water 30 times the weight of the dregs, and extract by reflux in a water bath at 100°C for 3 times, each time for 1 h. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.

[0033] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock

[0034] Weigh 3 g of the crude po...

Embodiment 2

[0037] Example 2 Determination of total sugar content and degree of substitution of hollyhock stem and leaf polysaccharide sulfated modification product

[0038] 1, with D-glucose anhydrous as standard substance, adopt the phenol-sulfuric acid method to measure the total sugar content of the crude polysaccharide of hollyhock stem and leaf before and after sulfation modification; 2 SO 4As a standard product, the barium chloride-gelatin turbidimetric method was used to measure the sulfate content, and the degree of substitution (DS) was calculated according to the following formula.

[0039]

[0040] In the formula: S% is the content of sulfur element

[0041] 2. Monosaccharide composition analysis

[0042] Using trifluoroacetic acid hydrolysis, PMP derivatized polysaccharide samples, using mixed monosaccharides as standard products, using high performance liquid chromatography to determine the composition of monosaccharides according to the peak time, and drawing the stand...

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PUM

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Abstract

The invention discloses an abelmoschus manihot stem leaf polysaccharide sulfating modified product having an immune activity improving function and a preparation method thereof. The sulfate group content in the sulfating modified product is 24.79%, the polysaccharide content is 52.10%, and polysaccharide is prepared from mannose, glucose, galactose and arabinose by the mole ratio of 0.45 to 18.96 to 1.0 to 0.53. An extraction separation process is optimized through a large number of experiments, the crude polysaccharide is obtained by adopting a water extraction and alcohol precipitation method, deproteinization is performed, then DEAE-52 cellulose resin is adopted for purification, high-purity abelmoschus manihot stem leaf polysaccharide is prepared, and then modification is performed by adopting sulfamic acid to obtain the abelmoschus manihot stem leaf polysaccharide sulfating modified product. Wasted abelmoschus manihot stem leaf resources are fully utilized and turned into wealth to obtain the abelmoschus manihot stem leaf polysaccharide sulfating modified product capable of improving the immune activity, sustainable application of traditional Chinese medicine resources can be achieved, and the abelmoschus manihot stem leaf polysaccharide sulfating modified product has a very good economic value and ecological environment protection significance.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to a sulfated modified product of hollyhock stem and leaf polysaccharide with the ability to improve immunity and a preparation method thereof. Background technique [0002] Traditional Chinese medicine resources are a solid foundation for safeguarding national health and developing ethnic medicine. In recent years, the production area of ​​traditional Chinese medicine and natural medicinal biological resources has exceeded 2.40×10 6 hm 2 , the output of medicinal materials can reach 5.40×10 6 t, while the biomass of discarded plant roots and aboveground stems and leaves is as high as 1.1×10 7 ~1.6×10 7 t is 2 to 3 times the output of medicinal materials, causing serious waste of resources and environmental pollution. Therefore, in the process of industrialization of traditional Chinese medicine resources, improving the utilization value of medicinal biological resources, especially en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C08B37/14C08B37/00A61K31/737A61P37/04A23L33/125
CPCA23V2002/00A61K31/737C08B37/0003C08B37/0009C08B37/0036C08B37/0087A23V2250/51A23V2250/618A23V2250/61A23V2250/608A23V2250/602A23V2300/14
Inventor 江曙潘欣欣段金廒朱悦钱大玮严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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