A kind of preparation method of active corn silk pure polysaccharide

A technology of corn silk and pure polysaccharides, which is applied in the field of preparation of active corn silk pure polysaccharides, can solve the problems of low polysaccharide content and no pure corn silk polysaccharides, and achieve the effect of rich resources and good immune regulation activity

Inactive Publication Date: 2017-11-10
QIQIHAR UNIVERSITY
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Problems solved by technology

[0005] At present, the biological activity research of corn silk polysaccharide mainly focuses on the crude polysaccharide, the polysaccharide content is low, and there is no pure corn silk polysaccharide

Method used

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  • A kind of preparation method of active corn silk pure polysaccharide

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Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of active corn silk pure polysaccharide, comprising the steps of:

[0021] 1. Pretreatment of corn silk

[0022] After removing impurities, the corn silk was dried at 65 °C, placed in a dry environment, cooled naturally, and then crushed to 60 mesh.

[0023] 2. Degreasing of corn silk

[0024] The corn silk powder was soaked in 95% ethanol or absolute ethanol at a mass volume ratio of 1:5 for 12 hours, filtered under reduced pressure, and the filter residue was soaked once more in the same way, then dried naturally in a cool place, and stored in a dry environment for later use.

[0025] 3. Preparation of Crude Polysaccharide

[0026] Add distilled water at a mass volume ratio of 1:15, and extract at 95 °C for 2 hours. The extract was centrifuged at 5000 r / min for 10 min, and the supernatant and precipitate were collected respectively. Precipitate and extract again in the same way. Combine the two extracts. The extract was subjected to high-spe...

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Abstract

The invention discloses a preparation method of pure active corn stigma polysaccharide and belongs to the technical field of extraction and separation of natural plant active polysaccharide. The pure active corn stigma polysaccharide is subjected to the processes of pretreatment and degreasing of corn stigma, preparation of crude polysaccharide and polysaccharide purification and is prepared by comprehensively adopting the ultrafiltration and ion chromatographic separation technology; the obtained pure corn stigma polysaccharide is homogeneous polysaccharide; the pure corn stigma polysaccharide prepared through the preparation method has excellent immunoregulatory activity according to discoveries of immunological competence researches.

Description

technical field [0001] The invention relates to a preparation method of active corn silk pure polysaccharide, which belongs to the technical field of extraction and separation of natural plant active polysaccharide. Background technique [0002] corn silk ( Stigmata madis ) alias corn wheat, cob hair, Gramineae maize ( Zea mays L． The style of ) is a traditional Chinese herbal medicine, which is recorded in "Southern Yunnan Materia Medica", "Dictionary of Chinese Medicine", etc., and is a commonly used medicinal material species included in the 1985 edition (Part 1) of "Ministry of Health Standards of the People's Republic of China". [0003] my country is a big corn producing country, and its annual corn production ranks second in the world. Therefore, the resource of corn silk as a by-product of corn is very abundant. [0004] Polysaccharides are one of the important chemical components contained in corn silk. It has been proved that the content of polysaccharides in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 宫春宇余世锋郑喜群王文侠张慧君
Owner QIQIHAR UNIVERSITY
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