A kind of stropharia polysaccharide and its preparation method and application

The technology of the polysaccharide and the polysaccharide is applied in the field of the polysaccharide and the preparation of the polysaccharide. The effect of enhancing phagocytic activity

Active Publication Date: 2022-06-21
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the fine structure, immunomodulatory activity and application of Stropharia polysaccharides

Method used

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  • A kind of stropharia polysaccharide and its preparation method and application
  • A kind of stropharia polysaccharide and its preparation method and application
  • A kind of stropharia polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Separation and extraction of the polysaccharide SR-1 of A. maxima

[0055] 1.1 Separation and extraction of polysaccharide SR-1

[0056] 1.1.1 Extraction of crude polysaccharide from Mushroom globulus by water extraction and alcohol precipitation

[0057] The pulverized M. globosa fruit body powder and distilled water were boiled in a water bath at 100° C. for 3 hours at a material-to-liquid ratio (W / V) of 1:3, boiled 3 times, and the supernatant was collected and concentrated to 200 mL. Three volumes of absolute ethanol were added to form a flocculent precipitate, which was collected and dried. The Sevage method was used to remove the protein in the extract to obtain the crude polysaccharide of Mushroom.

[0058] 1.1.2 Separation and Purification of Crude Polysaccharide of P. globosa

[0059]Weigh 50.00 g of DEAE-cellulose, stir evenly with distilled water, and soak for 24 hours to remove suspended particles. Then soaked in 0.5mol / L NaOH solution for 4 ho...

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PUM

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Abstract

The invention discloses a stropharia polysaccharide, a preparation method and application thereof. The polysaccharide is obtained from the fruiting bodies of Stropharia stropharii through water extraction and alcohol precipitation, protein removal, ion exchange column chromatography and dialysis concentration, which are α-D-galactose, α-D-glucose and β-D-glucose A heteropolysaccharide composed of glucose, wherein the molar ratio of α‑D‑galactose to glucose (including α‑D‑glucose and β‑D‑glucose) residues is 3:1, and α‑D‑glucose to β‑D‑glucose The molar ratio of glucose residues is 2:1. The polysaccharide has significant immunomodulatory activity.

Description

technical field [0001] The invention belongs to the technical field of application of fungal polysaccharides, in particular to a polysaccharide of A. maxima and a preparation method and application thereof. Background technique [0002] Polysaccharides are a class of biological macromolecules that are ubiquitous in living organisms. Modern biochemical studies have found that polysaccharides can participate in the cytoskeleton and some biologically active molecules; such as cellulose and chitin that maintain cell structure; starch, glycogen and other substances that provide energy sources. The development of polysaccharide is relatively late, and its structure is complex and diverse, so it is difficult to study its structure and activity. [0003] In the past 20 years, the research reports on the biological activities of polysaccharides mainly focus on the aspects of immune regulation, anti-tumor, anti-virus, anti-oxidation and hypoglycemia, and their effects are multi-chann...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00A23L33/125
CPCC08B37/006A61P37/04A61P35/00A23L33/125A23V2002/00A23V2200/324A23V2200/308
Inventor 侯怡铃丁祥蒋琳
Owner CHINA WEST NORMAL UNIVERSITY
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