Lyophyllum decastes cultivation method
A cultivation method, the technology of velvet antler mushroom, is applied in the fields of mushroom cultivation, cultivation, plant cultivation, etc. It can solve the problems of long preparation cost of solid strains, slow germination speed of strains, and high pollution rate of culture bottles, so as to improve the effect of germination and Yield, fast germination speed, and the effect of improving the budding rate
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Embodiment 1
[0016] A kind of preparation method of velvet mushroom liquid strain:
[0017] (1) Weigh the culture medium raw materials by mass parts: 40 parts of sawdust, 10 parts of rice bran, 5 parts of bran, 10 parts of cotton seed husk, 1 part of lime, 10 parts of nutrient soil, and 80 parts of water;
[0018] (2), mix the raw materials in step (1), control the pH value to 6, put into culture bottles, 400 parts per bottle, and seal with a cap;
[0019] (3) The culture bottle is sent to the vacuum sterilizer, and after sterilization by ozone for 1 hour, the vacuum degree is 50KPa, and the sterilization is performed at 150 ℃ for 100 minutes;
[0020] (4) Cool down at 15°C, add 1 part of humic acid, and send it into each bottle to receive 40 of the original seed after cooling;
[0021] (5) Cultivate for 20 days in a 20°C culture room with a humidity of 70% and a carbon dioxide concentration of 1600PPM;
[0022] (6) Scratching bacteria treatment, using the bacteria scraping machine to ca...
Embodiment 2
[0025] A kind of preparation method of velvet mushroom liquid strain:
[0026] (1) Weigh the raw materials of the culture medium in parts by mass: 60 parts of sawdust, 20 parts of rice bran, 10 parts of bran, 20 parts of cotton seed husks, 3 parts of lime, 15 parts of nutrient soil, and 110 parts of water;
[0027] (2), mix the raw materials in step (1), control the pH value to 7, put into culture bottles, 500 parts per bottle, and seal with a cap;
[0028] (3) The culture bottle is sent to the vacuum sterilization pot, and after 2 hours of ozone sterilization, the vacuum degree is 80KPa, and the sterilization is performed at 150 ℃ for 120 minutes;
[0029] (4) Cool at 20°C, add 2 parts of humic acid, and after cooling, send each bottle to receive 50 of the original seed;
[0030] (5) Cultivate for 25 days in a 25°C culture room with a humidity of 80% and a carbon dioxide concentration of 3200PPM;
[0031] (6) Scratching bacteria treatment, using the bacteria scraping machin...
Embodiment 3
[0035] A kind of preparation method of velvet mushroom liquid strain:
[0036] (1), weigh the raw materials of the medium by mass parts: 50 parts of sawdust, 15 parts of rice bran, 8 parts of bran, 15 parts of cotton seed husk, 2 parts of lime, 13 parts of nutrient soil, and 90 parts of water;
[0037] (2), mix the raw materials in step (1), control the pH value to 6, put them into culture bottles, 450 parts per bottle, and seal them with a cap;
[0038] (3) The culture bottle is sent to a vacuum sterilizer, sterilized by ozone for 1.5 hours, and then vacuumized at 60KPa, and sterilized at 150°C for 110 minutes;
[0039] (4) Cool down at 18°C, add 4 parts of humic acid, and send it into each bottle to receive the original seed 46 after cooling;
[0040] (5) Cultivate for 22 days in a 22°C culture room with a humidity of 78% and a carbon dioxide concentration of 2200PPM;
[0041] (6) Scratching bacteria treatment, using the bacteria scraping machine to carry out the bacteria ...
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