Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method
A technology of liquid culture medium and antler mushroom, applied in botany equipment and methods, liquid fertilizer, animal carcass fertilizer, etc., can solve the problems of long preparation cost of solid bacteria, slow germination speed of bacteria, high pollution rate of culture bottle, etc. To achieve the effect of improving the effect and output of bacteria, the speed of bacteria, and the effect of convenient production
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Embodiment 1
[0021] A liquid culture medium for antler mushrooms, comprising 10 parts by mass of sucrose, 2 parts of soybean meal, 1 part of beef extract, 0.3 part of magnesium sulfate heptahydrate, 0.3 part of potassium dihydrogen phosphate, and 0.1 part of a defoamer.
[0022] The preparation method of antler mushroom liquid culture medium comprises the following steps:
[0023] (1) Weigh the raw materials of the culture medium in parts by mass: 10 parts of sucrose, 2 parts of soybean meal, 1 part of beef extract, 0.3 part of magnesium sulfate heptahydrate, 0.3 part of potassium dihydrogen phosphate, and 0.1 part of defoamer;
[0024] (2) Mix the raw materials in step (1), control the pH value to 5.5, put them into culture bottles, 400 parts per bottle, and seal them with a gland;
[0025] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 1 hour, then vacuumize to 50KPa, keep warm at 115°C for 30 minutes, and keep warm at 121°C for 60 minutes.
[0026] The pr...
Embodiment 2
[0033] A liquid culture medium for antler mushrooms, comprising 30 parts by mass of sucrose, 5 parts of soybean meal, 3 parts of beef extract, 1 part of magnesium sulfate heptahydrate, 1 part of potassium dihydrogen phosphate, and 0.3 parts of an antifoaming agent.
[0034] The preparation method of antler mushroom liquid culture medium comprises the following steps:
[0035] (1) Weigh the raw materials of the culture medium in parts by mass: 30 parts of sucrose, 5 parts of soybean meal, 3 parts of beef extract, 1 part of magnesium sulfate heptahydrate, 1 part of potassium dihydrogen phosphate, and 0.3 parts of defoamer. ;
[0036] (2) Mix the raw materials in step (1), control the pH value to 6.5, put it into a culture bottle, 500 parts per bottle, and seal it with a gland;
[0037] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 2 hours, vacuumize to 80KPa, keep warm at 115°C for 30 minutes, and keep warm at 121°C for 60 minutes.
[0038] ...
Embodiment 3
[0046] A liquid medium for antler mushrooms, comprising 20 parts by mass of sucrose, 3.3 parts of soybean meal, 2 parts of beef extract, 0.5 parts of magnesium sulfate heptahydrate, 0.5 parts of potassium dihydrogen phosphate, and 0.1 parts of an antifoaming agent.
[0047] A preparation method for antler mushroom liquid culture medium, is characterized in that, comprises the following steps:
[0048] (1) Weigh the raw materials of the medium according to the parts by mass: 20 parts of sucrose, 3.3 parts of soybean meal, 2 parts of beef extract, 0.5 part of magnesium sulfate heptahydrate, 0.5 part of potassium dihydrogen phosphate, and 0.1 part of defoamer;
[0049] (2) Mix the raw materials in step (1), control the pH value to 6, put them into culture bottles, 500 parts per bottle, and seal them with a gland;
[0050] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 2 hours, vacuumize to 70KPa, keep warm at 115°C for 30 minutes, and keep warm ...
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