Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method

A technology of liquid culture medium and antler mushroom, applied in botany equipment and methods, liquid fertilizer, animal carcass fertilizer, etc., can solve the problems of long preparation cost of solid bacteria, slow germination speed of bacteria, high pollution rate of culture bottle, etc. To achieve the effect of improving the effect and output of bacteria, the speed of bacteria, and the effect of convenient production

Inactive Publication Date: 2015-03-11
江苏菇本堂生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2008, Juegouxun Forestry Farm of Iturihe Forestry Bureau, Yakeshi City, Inner Mongolia Autonomous Region applied for a patent for the cultivation method of lotus-leafed mushrooms (antler mushrooms). The cultivation met...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A liquid culture medium for antler mushrooms, comprising 10 parts by mass of sucrose, 2 parts of soybean meal, 1 part of beef extract, 0.3 part of magnesium sulfate heptahydrate, 0.3 part of potassium dihydrogen phosphate, and 0.1 part of a defoamer.

[0022] The preparation method of antler mushroom liquid culture medium comprises the following steps:

[0023] (1) Weigh the raw materials of the culture medium in parts by mass: 10 parts of sucrose, 2 parts of soybean meal, 1 part of beef extract, 0.3 part of magnesium sulfate heptahydrate, 0.3 part of potassium dihydrogen phosphate, and 0.1 part of defoamer;

[0024] (2) Mix the raw materials in step (1), control the pH value to 5.5, put them into culture bottles, 400 parts per bottle, and seal them with a gland;

[0025] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 1 hour, then vacuumize to 50KPa, keep warm at 115°C for 30 minutes, and keep warm at 121°C for 60 minutes.

[0026] The pr...

Embodiment 2

[0033] A liquid culture medium for antler mushrooms, comprising 30 parts by mass of sucrose, 5 parts of soybean meal, 3 parts of beef extract, 1 part of magnesium sulfate heptahydrate, 1 part of potassium dihydrogen phosphate, and 0.3 parts of an antifoaming agent.

[0034] The preparation method of antler mushroom liquid culture medium comprises the following steps:

[0035] (1) Weigh the raw materials of the culture medium in parts by mass: 30 parts of sucrose, 5 parts of soybean meal, 3 parts of beef extract, 1 part of magnesium sulfate heptahydrate, 1 part of potassium dihydrogen phosphate, and 0.3 parts of defoamer. ;

[0036] (2) Mix the raw materials in step (1), control the pH value to 6.5, put it into a culture bottle, 500 parts per bottle, and seal it with a gland;

[0037] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 2 hours, vacuumize to 80KPa, keep warm at 115°C for 30 minutes, and keep warm at 121°C for 60 minutes.

[0038] ...

Embodiment 3

[0046] A liquid medium for antler mushrooms, comprising 20 parts by mass of sucrose, 3.3 parts of soybean meal, 2 parts of beef extract, 0.5 parts of magnesium sulfate heptahydrate, 0.5 parts of potassium dihydrogen phosphate, and 0.1 parts of an antifoaming agent.

[0047] A preparation method for antler mushroom liquid culture medium, is characterized in that, comprises the following steps:

[0048] (1) Weigh the raw materials of the medium according to the parts by mass: 20 parts of sucrose, 3.3 parts of soybean meal, 2 parts of beef extract, 0.5 part of magnesium sulfate heptahydrate, 0.5 part of potassium dihydrogen phosphate, and 0.1 part of defoamer;

[0049] (2) Mix the raw materials in step (1), control the pH value to 6, put them into culture bottles, 500 parts per bottle, and seal them with a gland;

[0050] (3) Put the culture bottle into a vacuum sterilizer, sterilize with ozone for 2 hours, vacuumize to 70KPa, keep warm at 115°C for 30 minutes, and keep warm ...

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Abstract

The invention relates to a lyophyllum decastes liquid medium, a preparation method thereof, and a liquid spawn preparation method. The lyophyllum decastes liquid medium comprises the following components in parts by weight: 10-30 parts of cane sugar, 2-5 parts of soybean meal, 1-3 parts of beef extract, 0.3-1 part of magnesium sulfate heptahydrate, 0.3-1 part of monopotassium phosphate and 0.1-0.3 part of a defoaming agent. The lyophyllum decastes liquid medium is convenient to manufacture with low cost; in a liquid spawn preparation process, the spawn running speed of the liquid spawn is high, no pollution exists, no old mycoderma generates, and the improvement of the spawn running effect and the yield is facilitated.

Description

technical field [0001] The invention relates to a liquid culture medium of velvet antler mushroom, a preparation method thereof, and a preparation method of liquid strains. Background technique [0002] Antler mushroom, also known as Lyophyllum decastes (Fr. : Fr.) Sing.), lotus leaf mushroom, also known as Lengxiang fungus, a nest of sheep, Beifeng fungus, etc. in Yunnan, my country, is a basidiomycete Subphylum, Phylomycetes, Agarita, Agaricaceae, Agaricus, known as fried chicken mushroom in Europe, the meat is thick, delicate, fragrant and delicious, research shows that the crude protein, The amino acid content is high, and the fat content is low; it also contains trace elements zinc, copper and selenium that are beneficial to the human body and a large amount of vitamins B1, B2, B6, B12 and niacin, and has high nutritional value. According to modern scientific research, the β-glucan contained in pilose antler mushroom has anti-tumor effect after long-term consumption. ...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
CPCC05B17/00C05G5/20C05D5/00C05F1/00C05F5/002C05F11/00
Inventor 贲伟东杨仁智向银春
Owner 江苏菇本堂生物科技股份有限公司
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