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Method for small-scale production of selenium-rich lyophyllum decastes mycelium and application

A technology of lotus leaf phymeji and mycelium, which is applied in the field of small-scale production of selenium-enriched lotus leaf lyme mycelium, can solve problems such as immaturity, and achieve improved biological activity, increased yield, and improved safety sexual effect

Pending Publication Date: 2021-09-17
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the large-scale technology of artificial selenium-enriched cultivation of lotus leaf pleil mushroom is not very mature. How to realize the large-scale production of selenium-enriched lotus leaf phyme mycelium is an important topic that the industry has been constantly exploring

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  • Method for small-scale production of selenium-rich lyophyllum decastes mycelium and application
  • Method for small-scale production of selenium-rich lyophyllum decastes mycelium and application
  • Method for small-scale production of selenium-rich lyophyllum decastes mycelium and application

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Embodiment 1

[0031] A method for small-scale production of selenium-enriched lotus leaf phyllomy mycelia, comprising the following steps:

[0032] S1. Preparation of the lotus-leaf mushroom seed culture solution: In a 250mL Erlenmeyer flask, add 100mL of enriched PDA medium, insert the activated lotus-leaf mushroom strain, and culture it on a shaking table for 5 days to obtain the lotus-leaf mushroom The seed culture solution was placed in a 4°C refrigerator for later use;

[0033] S2. Preparation of protein hydrolyzate: Weigh 100 g of soybeans, soak them overnight, beat, boil and cool to 40°C, add 5% protease, and bathe in a constant temperature water bath at 40°C for 5 hours to prepare protein hydrolyzate;

[0034] S3. Preparation of cornstarch hydrolyzate: Weigh 600g of cornflour, add 6000g of hot water at 65°C, then add 5% amylase, place in a constant temperature water bath at 65°C and mix until it becomes colorless with iodine solution to obtain cornstarch hydrolyzate liquid;

[003...

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Abstract

The invention discloses a method for small-scale production of selenium-rich lyophyllum decastes mycelium and application, and relates to the technical field of microbial fermentation. The method comprises the steps that protein hydrolysate, corn starch hydrolysate and glucose are used as a fermentation culture medium, a lyophyllum decastes seed culture solution is inoculated into the fermentation culture medium, a sterilized Na2SeO3 solution is added in the culture process, and culture parameters are adjusted, so that the lyophyllum decastes mycelium with higher selenium content is obtained. According to the method for small-scale production of the selenium-rich lyophyllum decastes mycelium, the mycelium yield is greatly increased, the selenium-rich lyophyllum decastes mycelium can be continuously produced in a 20L biological fermentation tank, and the product batch stability is high. The produced selenium-rich lyophyllum decastes mycelium can be used for preparing functional foods, medicines or cosmetics for reducing blood fat, reducing blood sugar and resisting oxidation.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a small-scale production method and application of selenium-enriched lotus-leaf phyllum mycelium. Background technique [0002] As an essential trace element for the human body, selenium can stimulate the production of human immunoglobulins and antibodies, and has anti-aging effects. The lotus leaf lily mushroom is a fungus of the genus of lily mushroom, which has high nutritional value. Because of the strong selenium-enriching ability of the lotus leaf pleura, inorganic selenium can be safely and effectively converted into organic selenium polysaccharides and selenoproteins, which greatly improves the nutritional value and application range of edible fungi. [0003] At present, the large-scale technology of artificial selenium-enriched cultivation of the lotus leaf pleil mushroom is not very mature. How to realize the method of large-scale production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L31/00A23L33/00A61K8/99A61K36/06A61P3/06A61P3/10A61P39/06A61Q19/00A61Q19/08C12R1/645
CPCC12N1/14A23L31/00A23L33/00A61K8/99A61K36/06A61Q19/00A61Q19/08A61P3/06A61P3/10A61P39/06A23V2002/00A23V2200/3262A23V2200/328A23V2200/30
Inventor 高慧娟王雪梅魏生龙梁倩倩王晓琴
Owner HEXI UNIV
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