Lyophyllum decastes polysaccharide extract and preparation method and application thereof

A technology of polysaccharides and extracts from velvet antler mushrooms, which can be used in drug combinations, pharmaceutical formulas, and medical preparations containing active ingredients. Increased yield and simple equipment

Inactive Publication Date: 2019-01-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the traditional hot water extraction method is simple to operate, the time is too long; ultrasonic-assisted extraction shortens the extraction time, but it still takes several hours; the complex enzyme extraction conditions are mild and impurities are easy to remove, but the enzyme itself is easily inactivated and the cost is high

Method used

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  • Lyophyllum decastes polysaccharide extract and preparation method and application thereof
  • Lyophyllum decastes polysaccharide extract and preparation method and application thereof
  • Lyophyllum decastes polysaccharide extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 High-speed shear extraction of pilose antler polysaccharide

[0030] Take the dried antler mushroom fruiting body, crush it, soak it in 4 times 95% ethanol overnight, filter and collect the filter residue the next day, evaporate the reagents to obtain the degreased antler mushroom powder, and set aside;

[0031] Taking the polysaccharide content of velvet antler mushroom as an index, the influence of three factors of different solid-liquid ratio, rotation speed and extraction time on the polysaccharide content of velvet antler mushroom in high-speed shear extraction was explored, and the results are shown in Table 2;

[0032] Polysaccharide content (%) = total sugar content (%) - reducing sugar content (%)

[0033] Test No.

Ratio of solid to liquid mL / g

Speed ​​r / min

time min

Polysaccharide extraction rate (%)

1

15

9500

1

22.53

2

15

11500

2

22.34

3

15

14500

4

22.23

4

20

...

Embodiment 2

[0037] Example 2 Antler Mushroom Crude Polysaccharides LDS30, LDS60, LDS80—Determination of Reducing Power

[0038] Take the crude polysaccharides LDS30, LDS60, and LDS80 of antler mushroom obtained in Example 1, and make the crude polysaccharides LDS30, LDS60, and LDS80 into aqueous solutions of 0, 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mg / mL, respectively, for use;

[0039] Take 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg / mL of LDS30 each 1.0 mL in a test tube, add 1.0 mL of 0.2 mol / L phosphate buffer solution with pH = 6.6 and 1.0 mL of 1% ferricyanide Potassium solution, vortex and mix, keep warm at 50°C for 20min, then add 1.0mL of 10% TCA solution, add 1.0mL of 0.1% FeCl after cooling 3 Solution, react for 10min, measure the absorbance value A at 700nm x . Use an equal volume of distilled water instead of LDS30 as a blank control, and measure the absorbance value A 0 , V C As a positive control; LDS60, LDS80 operate as above. The reducing power formula is as follows:

[0040] Reduc...

Embodiment 3

[0043] Example 3 Deer antler mushroom crude polysaccharides LDS30, LDS60, LDS80——Determination of ABTS free radical scavenging ability

[0044] Measure 8.0mL of 7mmol / L ABTS solution and mix with 141μL of 140mmol / L potassium persulfate solution, and then react in the dark at 4°C for 12-16h. Dilute the stock solution with phosphate buffer solution of 10mmol / LpH=7.4 to OD=0.70±0.02 for later use;

[0045] Take 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2mg / mL of LDS30 each 1.0mL into a test tube, add 3.0mL ABTS free radical solution, mix well, react in the dark at 30°C for 5min, measure the absorbance at 734nm Value A i ; Use 1.0mL distilled water as a blank control, and measure the absorbance value A under the same conditions 0 ; Replace the ABTS free radical solution with 3.0mL distilled water as the sample control group, measure the absorbance value A j , V C As a positive control; LDS60, LDS80 operate as above. The formula of ABTS free radical scavenging ability is as follows:

[0...

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Abstract

: after smashing the dry product of a lyophyllum decastes fruiting body, soaking in 95% ethanol; after degreasing and drying, carrying out high-speed shearing extraction for 1 to 4 minutes at the ratio of materials to liquid being 1:15-25 and at the rotation speed of 9500 to 14500 rpm; centrifuging an extracting solution to discard precipitate, and collecting liquid supernatant; and after concentrating the liquid supernatant, carrying out grading alcohol precipitation, centrifuging, and carrying out freeze-drying to obtain the lyophyllum decastes polysaccharide extract. The lyophyllum decastespolysaccharide extract has obvious antioxidant activity and certain reducing power, can effectively remove ABTS free radicals and DPPH free radicals, and can be applied to the preparation of novel antioxidants. As lyophyllum decastes is safe and natural and has no toxic side effects, the lyophyllum decastes polysaccharide extract has good application prospect.

Description

(1) Technical field [0001] The invention relates to antler mushroom polysaccharide extract and its preparation method and application. (2) Background technology [0002] Antler mushroom, also known as Lyophyllum decastes., belongs to Basidiomycetes, Agaricaceae, Tricholomaceae, and Lyophyllum genus. It is a large-scale wild fine fungus with both food and medicine. Mainly eaten fresh, the mushroom meat is thick and delicate, fragrant, crispy and delicious, and the nutrition and taste remain unchanged after drying. It is called "Friedchicken mushroom" in Europe. Studies have shown that the content of crude protein and amino acids in the fruiting bodies of velvet antler mushrooms is high, and the content of fat is low. It also contains trace elements zinc, selenium and copper that are beneficial to the human body and a large amount of vitamin B 1 , Vitamin B 2 , Vitamin B 6 , Vitamin B 12 And niacin, which has high nutritional value. At the same time, its fruiting body can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P39/06
CPCA61K31/715A61K36/07A61P39/06C08B37/0003
Inventor 张安强秦春青姚垚瑜
Owner ZHEJIANG UNIV OF TECH
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