Intestinal symbiotic bacteria for enhancing baculovirus resistance of spodoptera frugiperda and application of intestinal symbiotic bacteria

Abstract

The invention discloses an intestinal symbiotic bacterium for enhancing baculovirus resistance of spodoptera frugiperda and application thereof, and relates to the technical field of microorganisms, the intestinal symbiotic bacterium is derived from the midgut of the spodoptera frugiperda, is separated and purified and is subjected to 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing identification, the sequence length is 1397 bp, and the sequence homology with the known Glutamicibacter soli is 99.93%. The strain is preserved in Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No.34972. The invention provides intestinal symbiotic bacteria for enhancing baculovirus resistance of spodoptera frugiperda and application of the intestinal symbiotic bacteria, and provides an experimental basis for further revealing an interaction mechanism among a host, a pathogen and the symbiotic bacteria. And meanwhile, a technical basis is provided for realizing a comprehensive virus prevention and control strategy of targeted biological prevention and control and non-target beneficial insect protection of the lepidoptera pests.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial technology, more particularly to a kind of intestinal symbiotic bacteria for enhancing the function of Spodoptera frugiperda against baculovirus and its application. BACKGROUND

[0002] Spodoptera frugiperda, as an invasive agricultural pest with polyphagous characteristics, has caused serious crop yield reduction in many regions of the world and caused huge economic losses. Baculovirus has become an important alternative to chemical pesticides due to its environmentally friendly characteristics. However, in practical application, baculovirus faces many challenges. First, the target pest population may gradually develop virus resistance under long-term virus selection pressure, thereby reducing the control effect. Second, baculovirus may infect and kill non-target economic beneficial insects such as Bombyx mori. Therefore, how to enhance the specific pathogenic ability of baculovirus to target pests while avoiding its negative effects on beneficial insects is a key problem that needs to be solved in the field of biological control.

[0003] Baculovirus has the characteristics of activating itself in the host intestine, specifically invading intestinal epithelial cells, completing initial replication, and breaking through the barrier to start systemic infection. However, whether the virus can successfully break through the intestinal barrier directly affects its replication and pathogenicity in the host body. At the same time, symbiotic microorganisms in insects, especially gut flora, play a key role in host nutrient metabolism, development and differentiation, and immune regulation. Studies have shown that gut symbiotic bacteria are necessary for the host to resist many pathogens. Therefore, regulating the composition of the target pest's gut flora has been considered an important strategy to improve virus infection sensitivity and overcome resistance. At the same time, this mechanism provides a theoretical basis for solving the problem of non-target beneficial insects infected with baculovirus: by optimizing the gut flora structure of economic beneficial insects such as Bombyx mori, an anti-virus "microecological barrier" can be constructed to effectively protect beneficial insects.

[0004] Based on the above research background, the research team conducted a systematic isolation and screening of the gut flora of Spodoptera frugiperda and successfully isolated a specific gut strain Glutamicibacter soli. This strain has the function of enhancing the host's resistance to baculovirus. This research result provides experimental support for revealing the interaction mechanism among host-pathogen-symbiotic bacteria, and lays a technical foundation for developing comprehensive virus control strategies for target biological control of lepidopteran pests and protection of non-target beneficial insects. SUMMARY

[0005] The present application aims at solving the problems in the background art, and provides an intestinal symbiotic bacteria for enhancing the baculovirus resistance of Spodoptera frugiperda and an application thereof.

[0006] In order to achieve the above-mentioned purpose, the present application specifically adopts the following technical solutions. The intestinal symbiotic bacteria for enhancing the baculovirus resistance of Spodoptera frugiperda is characterized in that the strain is derived from the midgut of Spodoptera frugiperda, is isolated and purified, and is identified by 16S rDNA gene sequencing, and the sequence length is 1397 bp, and the sequence homology with the known Glutamicibacter soli is 99.93%. The strain is preserved in the Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 34972, and the preservation time is June 23, 2025.

[0007] Preferably, the strain has enhanced resistance of Spodoptera frugiperda larvae to Spodoptera frugiperda nuclear polyhedrosis virus (SfMNPV) infection.

[0008] Preferably, the intestinal flora structure is suitable for regulating non-target economic beneficial insects, so as to reduce the infection sensitivity of the non-target economic beneficial insects to baculovirus.

[0009] As the same inventive concept as the above technical solutions, the present application also claims a method for evaluating the SfMNPV resistance function of the Glutamicibacter soli strain, using the above-mentioned intestinal symbiotic bacteria for enhancing the baculovirus resistance of Spodoptera frugiperda, comprising the following steps: (1) obtaining Spodoptera frugiperda larvae without intestinal bacteria by feeding artificial feed containing antibiotics; (2) re-implanting the Glutamicibacter soli strain into the intestines of the above-mentioned sterile larvae; (3) orally infecting the normal intestinal flora group, the intestinal bacteria-free group and the Glutamicibacter soli re-implantation group of larvae with SfMNPV; (4) monitoring the virus mortality rate, median lethal time (LT 50 ) and virus gene expression level of each group of larvae, so as to evaluate the influence of the strain on the antiviral ability of the host.

[0010] Preferably, the SfMNPV infection-induced mortality of the larvae is significantly reduced, the median lethal time is prolonged, and the expression level of the virus polyhedrin gene is inhibited.

[0011] As the same inventive concept as the above technical solutions, the present application also claims an application of the intestinal symbiotic bacteria for enhancing the baculovirus resistance of Spodoptera frugiperda, using the above-mentioned intestinal symbiotic bacteria for enhancing the baculovirus resistance of Spodoptera frugiperda.

[0012] Preferably, the intestinal symbiotic bacteria for enhancing the baculovirus resistance function of Spodoptera frugiperda is used to prepare the preparation and fermentation culture.

[0013] Preferably, the intestinal symbiotic bacteria for enhancing the baculovirus resistance function of Spodoptera frugiperda of claim 1 is used to prepare the insect microecological regulation material with baculovirus resistance function.

[0014] Compared with the prior art, the application provides the intestinal symbiotic bacteria Glutamicibacter soli of Spodoptera frugiperda and its application in enhancing the resistance of the host to baculovirus infection, and has the following beneficial effects: 1. The bacterial strain Glutamicibacter soli with clear baculovirus resistance function is screened and identified from the intestinal tract of Spodoptera frugiperda for the first time. The strain can realize stable colonization in the intestinal tract of insects, and provides a new biological resource and strategy for regulating the microecological environment of economic beneficial insects and carrying out virus prevention and control.

[0015] 2. The biological function of the Glutamicibacter soli strain is verified through in vivo virus infection experiments. When the larvae without intestinal bacteria are re-planted with the strain, the mortality rate of the virus-infected larvae is significantly reduced, the median lethal time is prolonged, and the virus gene expression level is also significantly reduced. This result shows that the strain can enhance the resistance of Spodoptera frugiperda to SfMNPV.

[0016] 3. The application uses the three-step method of antibacterial sterilization, target strain re-planting and virus infection to construct an experimental model, which actually verifies the resistance effect of the strain on baculovirus and has significant repeatability and biological function characteristics.

[0017] 4. The application further expands the interaction mechanism among the host, pathogen and symbiotic bacteria, and provides experimental basis for realizing the targeted biological prevention and control of lepidopteran pests.

[0018] 5. The Glutamicibacter soli strain provided by the application shows excellent culture performance and significant biological activity stability. This characteristic lays a solid technical foundation for its application in the field of precise targeted biological prevention and control of lepidopteran pests. At the same time, the strain also performs well in protecting non-target beneficial insects, providing strong support for constructing comprehensive virus prevention and control strategies. In addition, the Glutamicibacter soli strain has the potential for subsequent preparation development, providing reliable technical support for its commercialization. The strain not only has an important protective effect in the agricultural ecosystem, but also shows broad economic prospects, and is expected to bring significant benefits to the sustainable development of agriculture. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 A phylogenetic tree of the target bacterial strains in the intestine of Spodoptera frugiperda constructed based on 16S rDNA sequence similarity in the present application; Figure 2 A survival curve of Spodoptera frugiperda larvae infected with SfMNPV in different treatment groups in the present application; Figure 3 A viral gene expression amount in the midgut of Spodoptera frugiperda larvae infected with SfMNPV in different treatment groups in the present application. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] (I) A method for isolating and identifying Glutamicibacter soli, a symbiotic bacterium in the intestine of Spodoptera frugiperda 1. Strain isolation and purification Healthy 4th instar Spodoptera frugiperda larvae continuously bred for more than 30 generations in the laboratory were selected, the midgut tissues were taken under sterile conditions after surface disinfection, ground and gradiently diluted, and then inoculated on LB and NA solid culture media, and cultured at 37°C for 24 to 48 hours. Different morphological single colonies were selected, and the cultivable single strain was obtained by continuous streaking and purification, and stored in a -80°C ultra-low temperature refrigerator containing glycerol; 2. Molecular identification and phylogenetic analysis of the strain The genomic DNA of the single strain was extracted, PCR amplified and sequenced using 16S rDNA universal primers, and the obtained sequence was analyzed by BLAST comparison in the NCBI database to determine the species classification of the cultivable strain. The comparison result shows that the target strain is Glutamicibacter soli, and the 16S rDNA sequence similarity with the known sequence is 99.93%. Further based on the 16S rDNA sequence, a phylogenetic tree was constructed by the neighbor-joining method (NJ) of MEGA 6.0 software to analyze the evolutionary relationship.

[0022] (II) Construction of germ-free bacterial larvae model and re-implantation of target strain Penicillin (300 U / g) and streptomycin (300 μg / g) were uniformly mixed into artificial feed to feed the newly hatched larvae to the end of the 2nd instar stage to eliminate the intestinal flora, and the germ-free insect body was constructed. Then the cultured Glutamicibacter soli strain was collected in the logarithmic growth phase, adjusted to OD600 = 2.0 were prepared into high-concentration bacterial suspensions and uniformly added to the feed, which was fed to 3rd instar larvae after antibiotic treatment to 4th instar larvae, to ensure that the strains successfully colonized the host gut and established a single-strain gut ecological model.

[0023] (Three) Baculovirus infection method and lethal effect monitoring Standard virus infection experimental system was constructed: SfMNPV virus suspension (1 x 10 8 OBs / mL) preserved and quantitatively purified in the laboratory was uniformly coated on the surface of artificial feed and naturally dried for infection. The insect bodies of each experimental group (normal gut bacteria group, no gut bacteria group, Glutamicibacter soli reimplantation group) were transferred to virus-treated feed for free feeding for 1 hour to complete a one-time oral infection. After infection, the larvae were continuously raised under constant conditions, and fresh feed was replaced daily to avoid cross contamination. The state of insect bodies in each group was continuously monitored, and the death determination was based on the softening, disintegration and typical virus lysis symptoms of insect bodies.

[0024] (Four) Functional verification method of Glutamicibacter soli against baculovirus 1. Virus virulence monitoring From the 4th day of infection, the number of deaths was recorded daily at regular intervals until all larvae developed or died. GraphPad Prism software was used to calculate the mortality rate and median lethal time (LT 50 ) of each treatment group, and Log-rank test was used for survival analysis comparison.

[0025] 2. Virus replication capacity determination qPCR technology was used to detect the expression level of virus polyhedrin gene in the midgut tissue of different treatment groups of Spodoptera exigua, to evaluate the regulation of intestinal bacteria on virus replication.

[0026] Example One, isolation and purification of Spodoptera exigua intestinal bacteria The Spodoptera frugiperda collected from Guangdong Province was continuously bred for more than 30 generations in the laboratory with artificial feed, and the breeding conditions were as follows: temperature 27 ± 1 ℃, relative humidity 70% ± 5%, and light cycle L∶D = 12 h∶12 h. 20 healthy and consistent 4th instar larvae were selected, washed with sterile water for three times, immersed in 75% ethanol for 2 min, and then washed with sterile water for three times. The larvae were placed in a sterile culture dish and dissected in a clean bench, and the midgut was quickly taken out and placed in a 1.5 mL sterile centrifuge tube. After adding an appropriate amount of sterile water, the midgut tissue was ground into homogenate, and then sterile water was added to make the volume to 1 mL, and then mixed thoroughly. Centrifuged at 5000 r / min for 1 min, the precipitate was discarded, and the supernatant was used as the midgut stock solution. The stock solution was sequentially gradient diluted, 100 μL of each dilution gradient was uniformly coated on LB solid medium (10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 20 g of agar, and 1 L of water) and NA solid medium (3 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar, and 1 L of water), and three replicates were set for each dilution. The culture dishes were placed in a 37 ℃ constant temperature incubator for 24 to 48 hours. Single colonies with different morphological characteristics were selected and purified by continuous streaking on freshly prepared LB solid medium for 5 times to obtain single strains. The purified strains were inoculated in 10 mL of LB liquid medium and cultured in a 37 ℃ constant temperature shaker at 180 r / min for 12 hours. After the culture was completed, 20% glycerol was added, mixed, and then stored in a-80 ℃ ultra-low temperature refrigerator. -2 -3 -4 -5 Gradient dilution, 100 μL of each dilution gradient was uniformly coated on LB solid medium (10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 20 g of agar, and 1 L of water) and NA solid medium (3 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar, and 1 L of water), and three replicates were set for each dilution. The culture dishes were placed in a 37 ℃ constant temperature incubator for 24 to 48 hours. Single colonies with different morphological characteristics were selected and purified by continuous streaking on freshly prepared LB solid medium for 5 times to obtain single strains. The purified strains were inoculated in 10 mL of LB liquid medium and cultured in a 37 ℃ constant temperature shaker at 180 r / min for 12 hours. After the culture was completed, 20% glycerol was added, mixed, and then stored in a-80 ℃ ultra-low temperature refrigerator.

[0027] Example Two, Molecular identification and phylogenetic analysis of cultivable intestinal bacterial strains ​​​The single strain of cultivable bacteria isolated from the intestine of Spodoptera frugiperda and obtained after purification was sent to Beijing Tianyihuiyuan Biotechnology Co., Ltd. for 16S rDNA gene sequencing analysis. The specific steps are as follows: first, the genomic DNA of each target strain was extracted, and PCR amplification was performed using 16S rDNA universal primers. The size and purity of the amplification product were verified by 1% agarose gel electrophoresis, and then the target band was recovered and purified. The purified product was subjected to bidirectional sequencing using an ABI3730-XL sequencer to obtain high-quality 16S rDNA sequence data. The measured sequence was submitted to the NCBI GenBank database, and homology comparison analysis was performed using the BLAST tool. In the comparison results, the reference sequence with a similarity (Sequence Identity) of ≥98% and the highest alignment score (Max Score) was used as the basis to determine the species attribution and classification status of the target strain. The sequencing sequence of Glutamicibacter soli is shown as SEQ ID NO. 1. Based on the 16S rDNA sequence, 20 strains of sequences most closely related to the target strain at the species level were selected, and a phylogenetic tree was constructed using the Neighbor-Joining (NJ) method by MEGA 6.0 software to analyze the phylogenetic relationship, wherein the target strain is numbered as IOZCAS-Sf-Gb.

[0028] SEQ ID NO. 1: Example Three, removal of Spodoptera frugiperda intestinal bacteria and single strain re-implantation method To study the influence of specific intestinal bacterial strains on the anti-baculovirus function of Spodoptera frugiperda, the original intestinal flora of the larvae was first removed by antibiotic treatment. The specific method is as follows: select newly hatched larvae, feed them with artificial feed containing antibiotics, and the antibiotics are mixed in the feed at a ratio of 300 U / g of penicillin and 300 μg / g of streptomycin. Continuous feeding until the end of the 2nd instar, dissect under aseptic conditions, prepare intestinal homogenate and perform plate dilution coating culture to confirm the acquisition of intestinal bacteria-free larvae individuals.

[0029] Subsequently, the re-implantation of the target strain was performed: the single bacterial strain to be re-implanted was inoculated into LB liquid medium and cultured in a constant temperature shaker at 37°C and 180 r / min for 12 hours to ensure that the bacteria were in the logarithmic growth phase. After the culture was completed, the bacteria were collected by centrifugation at 8000 r / min for 5 min, part of the supernatant was discarded, and the bacteria were resuspended to a final optical density (OD 600 ) of 2.0 to prepare a high-concentration bacterial suspension for standby. The above bacterial suspension was uniformly added to the artificial feed, and the 3rd instar larvae treated with antibiotics were fed, and the larvae were fed to the 4th instar. After the feeding was completed, the individuals were randomly sampled for intestinal flora detection to confirm the successful colonization of the target strain in the intestine, i.e., the Spodoptera frugiperda experimental material with specific strain reconstruction was obtained, which could be used for subsequent functional verification experiments.

[0030] Example Four, virus infection experiment and death monitoring method To verify the pathogenicity of specific intestinal bacterial strains on Spodoptera frugiperda nucleopolyhedrovirus, the following infection experiment and death monitoring process was designed and carried out: (I) Preparation of experimental materials Select healthy Spodoptera frugiperda 4th instar larvae with consistent development, and divide them into four treatment groups: control group, normal intestinal bacteria group, intestinal bacteria-free group, and single strain re-implantation group, with 3 replicates in each group, and each replicate containing 24 larvae. The virus used in the experiment is the SfMNPV strain preserved in the laboratory, which is purified and quantified in advance to prepare a virus suspension of 1×10 8 OBs / mL for standby.

[0031] (II) Infection method The virus suspension was uniformly coated on the surface of fresh artificial feed and allowed to dry naturally to ensure stable adhesion of the virus. Then the larvae in each group were transferred to the treated feed separately and allowed to feed freely for 1 hour to complete the one-time oral infection. The control group was not added with virus and served as a blank control.

[0032] (III) Post-infection feeding management and death monitoring After the end of infection treatment, the larvae were continuously reared under standard environmental conditions: temperature 27 ± 1 °C, relative humidity 70% ± 5%, photoperiod L:D = 12 h:12 h. Fresh food was replaced daily to avoid cross-contamination, and the larvae were continuously observed. The criteria for death caused by viral infection were: softening of the body surface, disintegration of the structure, and appearance of typical lytic phenomena.

[0033] Example Five, Method for determining the infection ability of Glutamicibacter soli on Spodoptera frugiperda nucleopolyhedrovirus To evaluate the regulatory effect of specific intestinal bacteria Glutamicibacter soli strain on SfMNPV infection of Spodoptera frugiperda, systematic analysis was carried out from two aspects of viral mortality rate and viral gene expression.

[0034] (I) Virulence monitoring From the 4th day after viral infection treatment, the survival status of larvae in each treatment group was observed and recorded daily at regular times, and the dead individuals were immediately removed and recorded. The observation was continued until all larvae completed development or died. The data of each treatment group were statistically analyzed using GraphPad Prism software, and the survival rate difference was compared by Log-rank test, and the mortality rate and median lethal time (LT 50 ) were calculated. The statistical results are as follows: the mortality rate of the normal intestinal bacteria group was 62.86%, the LT 50 was 8.16 days; the mortality rate of the no intestinal bacteria group was 94.29%, the LT 50 was 7.12 days; the mortality rate of the Glutamicibacter soli strain re-implantation group was 64.79%, the LT 50 was 8.54 days. The results showed that the removal of intestinal bacteria can significantly enhance the lethal ability of SfMNPV, and the re-implantation of Glutamicibacter soli can significantly alleviate this effect.

[0035] (II) Viral gene expression determination Real-time fluorescent quantitative PCR (qPCR) technology was used to detect the expression level of viral genes in different treatment groups.

[0036] 1. Experimental treatment and sample collection According to the method of Example 4, Spodoptera frugiperda 4th instar larvae were subjected to viral infection treatment. The midgut tissues were collected at 24 hours and 72 hours after infection for viral gene expression detection. Three biological replicates were set for each treatment group, and 10 larvae were collected for each replicate, and the samples were immediately stored in liquid nitrogen for preservation.

[0037] 2. Total RNA extraction and cDNA synthesis Total RNA of midgut was extracted using Trizol reagent, and RNA degradation was strictly prevented during operation. The purity and concentration of the RNA sample were detected by NanoDrop2000, and the A260 / A280 ratio was ensured to be between 1.8 and 2.1. The cDNA was synthesized using Evo M-MLV RT Mix Kit with gDNA Clean for qPCR Ver.2 kit (Hunan Aikangrui Biological Engineering Co., Ltd.).

[0038] 3. qPCR reaction system The SfMNPV late gene polyhedrin (polyh) was selected as the target gene, and the Spodoptera ribosomal protein L13 (RPL13) was selected as the internal reference gene. The primers used are as follows: RPL13 Forward: 5'-GCCTTAACCCTGCTTTTGCTAG-3' Reverse: 5'-GCTTCGCCCTTCAATACCTTC-3' polyh Forward: 5'-CCCACTAGGCCCAACAGATG-3' Reverse: 5'-ACGAGCGACAGTTCAAGGAG-3' The qPCR reaction was performed using SYBR Green Premix Pro Taq HS qPCR Kit (Hunan Aikangrui Biological Engineering Co., Ltd.), and the reaction system and program were performed according to the kit instructions. The relative expression of genes was calculated using 2 -ΔΔCt The results of the analysis showed that the expression of viral genes in the midgut increased significantly after removing intestinal bacteria, and the expression of viral genes decreased significantly after re-planting Glutamicibacter soli, indicating that Glutamicibacter soli had a certain inhibitory effect on the replication of viruses.

[0039] Based on the analysis of virus mortality, survival curve and virus gene expression level, the results showed that Glutamicibacter soli could significantly enhance the resistance of Spodoptera exigua to SfMNPV, which was manifested as a significant decrease in mortality, an increase in median lethal time and a decrease in virus gene expression level in the treatment group with Glutamicibacter soli. Therefore, Glutamicibacter soli plays a significant role in regulating the gut microbiota structure of Spodoptera exigua and improving its resistance to baculovirus infection. This strain can be applied to the construction of intestinal microecological regulation scheme for economic beneficial insects, which is consistent with the microbial intervention strategy in the green prevention and control technology system.

Claims

1. An intestinal symbiotic bacterium that enhances the resistance of fall armyworm to baculovirus, characterized in that, This strain was derived from the midgut of the fall armyworm. It was isolated, purified, and identified by 16S rDNA sequencing. The sequence length was 1397 bp, and it showed 99.93% homology with the known Glutamicibacter soli. The strain is deposited at the Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 34972.

2. The intestinal symbiotic bacteria that enhances the resistance of fall armyworm to baculovirus according to claim 1, characterized in that, The strain enhances the resistance of fall armyworm larvae to fall armyworm nucleopolyhedrovirus (SfMNPV) infection.

3. The intestinal symbiotic bacteria for enhancing the resistance of fall armyworm to baculovirus as described in claim 2, characterized in that, It is suitable for regulating the gut microbiota structure of non-target economically beneficial insects to reduce their susceptibility to baculovirus infection.

4. A method for evaluating the anti-SfMNPV function of Glutamicibacter soli strains, characterized in that, Using the gut symbiotic bacteria that enhances the resistance of fall armyworm to baculovirus as described in claim 1, the steps include: (1) Obtain fall armyworm larvae without intestinal bacteria by feeding them with antibiotic-containing artificial feed; (2) The Glutamicibacter soli strain was reintroduced into the intestine of the above-mentioned sterile larvae; (3) Oral infection of larvae of normal intestinal flora group, non-intestinal flora group, and Glutamicibacter soli replantation group with SfMNPV; (4) Monitor the virus lethality and median lethal time (LT) of larvae in each group. 50 The study also examined viral gene expression levels to assess the impact of the strain on the host's antiviral capacity.

5. The method for evaluating the anti-SfMNPV function of Glutamicibacter soli strain according to claim 4, characterized in that, It significantly reduced the mortality rate of parasites caused by SfMNPV infection, prolonged the median lethal time, and suppressed the expression level of the viral polyhedrin gene.

6. The application of an intestinal symbiotic bacterium that enhances the resistance of the fall armyworm to baculovirus, characterized in that, Using the gut symbiotic bacteria of claim 1 that enhances the anti-baculovirus function of fall armyworm.

7. The application of the intestinal symbiotic bacteria that enhances the resistance of fall armyworm to baculovirus according to claim 6, characterized in that, An intestinal symbiotic bacterium that enhances the fall armyworm's resistance to baculoviruses was used for formulation preparation and fermentation culture.

8. The application of the intestinal symbiotic bacteria that enhances the resistance of fall armyworm to baculovirus according to claim 6, characterized in that, The gut symbiotic bacteria of claim 1, which enhances the anti-baculovirus function of the fall armyworm, are used to prepare insect microecological regulation materials with anti-baculovirus function.

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