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Rapid evaluation method for salt tolerance of tomatoes

An evaluation method and technology of salinity tolerance, applied in the field of rapid evaluation of tomato salinity tolerance, can solve problems such as difficult application, and achieve the effect of large profit, high yield and high quality

Inactive Publication Date: 2014-06-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the prior art, the method for evaluating tomato salt tolerance is mainly through the tomato salt tolerance index, such as physiological indicators such as salt damage index and chlorophyll content, plasma membrane permeability (Li Ye, Jiang Jingbin, etc. Tomato seedling stage tolerance Screening research on salinity-related indicators. Northern Horticulture, 2009 (10): 84-86) for research, but these indicators are difficult to apply in actual production

Method used

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  • Rapid evaluation method for salt tolerance of tomatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Material planting

[0023] Select plump Pennelli tomatoes (from the Horticultural Department of the University of California, USA) and the seeds of tomato varieties such as Jiaonong No. 1, Jiao Nong No. 2, and "Xianfeng" to germinate, and broadcast the germinated seeds directly on the soil filled with peat: perlite. : Sand = 1:1:2 in a nutrient bowl, placed in a double-layer air-filled film greenhouse to grow seedlings. After the plants of Pennelli tomato and other tomatoes grow branches, cut the side branches with leaves of the same thickness and length for rooting.

[0024] (2) Tissue culture rooting

[0025] The tomato branches used for rooting are sterilized, and cuttages are placed on 1 / 2 MS medium under sterile conditions, and each 100g of MS medium contains 3.0g of sucrose, 0.8g of agar, pH5.8, at a temperature of 25 ± 1 ℃, light intensity 25μmol·m -2 ·s -1 , The photoperiod is 14h light / 10h dark condition. After the branches sprouted roots, the seawater...

Embodiment 2

[0038] (1) Material planting

[0039] Select full-bodied Pannelier tomatoes, Jiaonong No. 1, Jiaonong No. 2, "Xianfeng" and other tomato varieties for germination, and broadcast the germinated seeds in a container filled with peat:perlite:sand=1:1:2 In the nutrient bowl, place the seedlings in a double-layer inflatable film greenhouse. After the Pennelli tomato and other tomato plants grow branches, cut side branches of the same thickness and length for rooting.

[0040] (2) Hydroponic rooting

[0041] Sterilize the distilled water, put it in a jar, add an appropriate amount of Hoagland nutrient solution, and put the tomato branches for rooting into the jar after being sterilized. The bottom leaf axil should be submerged in the water. Carry out rooting culture. After the branches sprouted roots, the seawater culture experiment was carried out.

[0042] (3) Preparation and gradient experiment of artificial seawater and nutrient solution

[0043] Configuration of artificial s...

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Abstract

The invention discloses a rapid evaluation method for the salt tolerance of tomatoes. The rapid evaluation method is characterized in that wild tomatoes with very high salt tolerance, namely solanum pennellii LA716, are adopted as a reference substance, and the salt tolerance degree of a tomato material to be evaluated is compared with that of the solanum pennellii LA716 under different concentrations of artificial seawater for making quick judgment. The rapid evaluation method comprises the specific steps of: (1) planting the solanum pennellii LA716 and the tomato material to be evaluated; (2) carrying out tissue cultivation or water cultivation on the solanum pennellii LA716 and the tomato material to be evaluated; (3) carrying out a salt tolerance gradient experiment on the solanum pennellii LA716 and the tomato material to be evaluated; and (4) comparing and evaluating the salt tolerance conditions of the tomato material to be evaluated and the solanum pennellii LA716 so as to directly judge the salt tolerance sequence of various tomato materials. The rapid evaluation method for the salt tolerance of the tomatoes is simple and convenient, is easy to grasp and is suitable for rapidly identifying the salt tolerance degrees of tomato varieties; the rapid evaluation method is used for culture experiments and production to obtain high-output and high-quality products and the maximum benefit is obtained.

Description

technical field [0001] The invention relates to a quick evaluation method for tomato salinity tolerance, which can be used for salt-tolerant tomato breeding and planting and popularization of salt-tolerant varieties, and belongs to the technical field of crop breeding. Background technique [0002] Tissue culture technology is a mature technology commonly used in crop breeding. The whole plant is obtained by cutting out plant parts such as leaves, stems, and roots for aseptic culture. Hydroponics technology uses the regeneration ability of plants, selects leaves and branches, immerses them in sterile water for rooting, and promotes the growth of plants by injecting nutrient solution. [0003] Salinized soil is widely distributed in the world, accounting for one-third of the world's total land area (Zhai Fenglin, Cao Mingqing. Salt tolerance and improvement of plants. Beijing: Agricultural Press, 1989, 3-4). Salinity is one of the major environmental factors limiting plant g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 王新华陈火英刘杨
Owner SHANGHAI JIAO TONG UNIV
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