The invention relates to the technical field of
genetic engineering, in particular to bemisia tabaci
pectin methylesterase, a BtPME1
gene, dsRNA and application of the bemisia tabaci
pectin methylesterase to prevention and treatment of bemisia tabaci. The
amino acid sequence of the bemisia tabaci
pectin methylesterase provided by the invention is as shown in SEQ ID NO. 2. Based on bemisia tabaci
genome data in the early stage of a laboratory, a bemisia tabaci horizontal
transfer gene is analyzed, it is found that a
plant-derived horizontally-transferred pectin-like methylesterase coding
gene BtPME1 exists in bemisia tabaci, and the
amino acid sequence of pectin-like methylesterase expressed by the coding
gene BtPME1 is shown as SEQ ID NO.2. After the BtPME1 gene is silenced, the
survival rate and
egg laying amount of bemisia tabaci on a host
plant can be effectively reduced. The invention provides a new target for a bemisia tabaci green control technology based on
RNA interference guidance, and opens up a new way for improving the
insect resistance of crops.