98 results about "Transfer gene" patented technology

The invention belongs to the technical field of biology and particularly relates to preparation and cell transfection application of a CS-based (chitosan-based) and CRISPR/Cas9 plasmid-constructed nano gene drug. The preparation comprises dissolving chitosan into acetic acid solution, dropwise adding the chitosan acetic acid solution into water, adding in CRISPR in mass proportion to the chitosan,encapsulating and carrying CRISPR/Cas9 with the chitosan to prepare a novel gene drug nano particle for good gene transfection effects or targeted exogenous gene introduction and further to achieve the effects of transferring gene drugs into cells. The novel nano compound of CRISPR@CS structured on the CS and the CRIPPR through an optimal feeding ratio can significantly improve the transfection efficiency of genes in cells to convey more genes into the cells to take effects, thereby being expected to being widely applied in the field of gene treatment through gene drug delivery.

This invention discloses one EPSPS gene enzyme immune agent case and its use method in the anti-herbicide bean, wherein, the case comprises anti-EPSPS multi-clone antigen, enzyme board with the antigen, enzyme antigens for two, gene switch bean standard product, non-transfer gene bean standard, intense wash liquid, develop liquid and terminal liquid. This invention method comprises the following steps: cloning CP4-EPSPS gene from the gene switch bean expressed in the bacillus coli; then through protein purification complex property to regroup EPSPS protein antibody immune animal to get special single clone antigen and multi-clone antigen; establishing double antigen clamp enzyme immune system test sample to determine its EPSPS protein content.

The invention provides a universal yeast donor strain that contains a conditional centromere and a URA3 allele on every chromosome. This strain was constructed in four rounds of crosses of individual conditional chromosome strains using a novel tetrad-based screen to identify segregants in which all marked chromosomes were contained in the same spore. The invention also provides an improved high efficiency method to transfer extrachromosomal genetic material such as plasmid DNA into any Saccharomyces strain for use with the current gene disruption libraries. The method of transfer is mating-based method which uses a kar1 plasmid donor strain that can initiate mating but cannot form a diploid and allows plasmid transfer (plasmoduction) between nuclei in the heterokaryon. kar1 matings have been used to transfer YACs between yeast strains, but previous methods required specialized genetic backgrounds in the recipient strains and suffered from high rates of spurious chromosome transfer (Hugerat, Y., et al. 1994. Genomics 22:108). Plasmoduction with the universal donor strain only requires that the recipient strain be ura3, GAL+ and have another marker available for selection of the transferred plasmid. Counterselection against every donor chromosome also limits the amount of spurious allele transfer. The universal donor strain and the method of the invention are used to screen the yeast gene disruption library with plasmid-based dominant negative alleles of various genes.

This invention discloses a method for improving the salt resistance and drought resistance of the corn and wheat by aggregating the beta, NHX1, PPase genes with the transfer genes, the steps as follows: regrouping the solution film natrium hydrogen counter-operating protein gene NHX1, the solution film tar phosphatase gene PPase and the Cholinesterase dehydrogenase gene from the escherichia colim to the plant expression carrier, then adding the corn or wheet cells and make them express efficiently, then producing the transfer gene plant; screening out the transfer gene pure cell that has apparently higher salt resistance and drought resistance from the transfer gene plant and its offspring; getting the transfer gene plant with the three object genes through twice tranfering the transfer gene pure cell or making the plant with the different transfer genes mate mutully, then sceening out the ones that have better salt resistance and drought resistance in their offsprings, and making them mute and purify by themselves to creat the new corn and wheat seeds with the salt resistance and drought resistance; or making use of the transfer polymeric material to produce the mixing seeds directly.

This invention relates to a transfer gene soybean checking method and its eduction, which includes:1 distilling soybean DNA;2 PCR expanding reaction, the total volume of the system is 25ul, each component and its final concentration is as follows: cushion solution 10mM, dNTP0.2mMú¼Mg2+1.5mMú¼the up eduction and the down eduction are 0.2mMú¼Taq 1Uú¼DNA50ng respectively, the special eduction react under the condition of the special PCR reaction by filling ddH20 up to the 25ul; after the reaction ending, distills the systerm solution 10ul and makes electrophoresis detection with 2.0úÑ agarose gelatin, and makes record; 3judging whether the soybean is the transfer gene soybean according to the record that whether it can display a 337bp unusual zoster. The related matter includes the up eduction and the down matter, whichare:Fú‘5'-CACTGACGTAAGGGATGA-3 18nt Tmú‘55.0ú¼Rú‘5'-TGTGCTGTAGCCACTGAT-3'18nt Tmú‘55.0.

The invention discloses a method of cultivated silkworm transfer-gene, which comprises the following steps: formulating paste with forced fecula to fix silk seed; utilizing 70% alcohol to sterilize silk seed; arranging the silk seed one by one with fine writing brush; colonizing extra-source gene on the rotor seat carrier; constituting recombinant rotor seat; setting the mass ratio with Helper plasmid as 1 : 1; adjusting the density at 0.4 mu g/mu l with injection buffer liquid; carrying out microscopic injection with stereoscopic microscope; setting the injecting quantity at 15-20ng DNA; sealing with leather and gel; transferring the silk seed and slide into incubation vessel; incubating at 25 deg.c; hatching and seeding; choosing successive; checking.

The invention discloses a cotton salt-tolerant gene, i.e. Gossypium wild dry land cotton CIPK-like protein phosphokinase GarCIPK. The gene has the sequence of SEQ ID No.1 in a sequence list. A cotton stress tolerance related gene is separated by an electronic cloning and reverse transcription-polymerase chain reaction (RT-PCR) technology, and function verification is performed by transferring gene into tobacco and shows that the salt tolerance of a transgenic plant is obviously improved.

The invention discloses an alfalfa genetic inverting method in plant transferring gene technical domain, which comprises the following steps: 1, comprising two methods; evoking callus with alfalfa hypocotyls through floating culture mode; assisting by super sound; sieving body embryo in medium; germinating the body embryo; generating root; getting the transforming plant; 2, using tender true leaf of alfalfa as transferring acceptor material; transforming with agricillin medium guild; sieving in traumatic evoked medium; seeding resistance callus in differential medium; evoking out of body embryo; generating root; getting the transforming plant. This invention increases genetic transferring efficiency of alfalfa and shorten genetic transferring period.

The present invention provides for liposomal vectors which inhibit inflammation and/or enhance expression of transferred genes. It is based, at least in part, of the discovery that vectors comprising a nucleic acid carrying a gene of interest and a cationic liposome containing an immunosuppressive agent induce lower levels of inflammatory cytokine relative to conventional lipolex vectors, as well as on the discovery that a component may be incorporated into the liposome which enhances expression of the gene of interest.

The invention relates to a targeting gene transferring method of folic acid-functionalized PAMAM (polyamidoamine dendrimers) wrapped by gold nanoparticles. The targeting gene transferring method comprises the steps of preparing the folic acid-functionalized PAMAM wrapped by gold nanoparticles; preparing a carrier of the folic acid-functionalized PAMAM wrapped by gold nanoparticles and a plasmid compound; and transferring and expressing targeting cervical cancer cells of the compound. The carrier prepared by the invention is used for transferring genes, has the advantages of moderate transferring condition, easiness in operation, high transferring efficiency, good specificity and the like, and has good application prospects in the aspect of cancer treatment and the like.

A bivalent plant expression carrier for culturing high anti-roundup transfer gene plant contains G2-aroA and gat. It has better anti-roundup performance and can be used to culture various high anti-roundup transfer gene plants.

The invention deals with the method of cloning the big critter using the transferring human a-whey albumen gene, the handling process is showed as follows: (1) using whole Human a-whey albumen gene frame to construct the galactophore unusual expressing carrier, (2) constructing the in series fabric connecting the human a-whey albumen unusual expressing carrier and the double single choosing carrier and transmitting the in series fabric DNA to the body nucleolus to gain the transferring gene cell, (3) cloning the body cell using the transmitting gene cell for the nucleus feeding body to gain the transferring clone big critter containing the transferring human a-whey albumen. The transferring gene milch cow galactophore unusually expressing recombining human a-whey albumen can be tapped the health caring drug and the leechdom, the milk containing the recombining human a-whey albumen can be right tapped to the health care milk and the milk produce with the high appending value.

A method for converting Chinese ephedra total DNA into yeasts, the transfer gene yeasts thereof, a culture medium for separating the transfer gene yeasts and liquid culture medium for transfer gene yeasts are disclosed.

The invention discloses a method for forecasting genes transferring horizontally in a genome by utilizing a bionic pattern identification principle, which comprises the steps of: utilizing the homologous continuity-based bionic pattern identification principle to forecast the gene transferring horizontally in genome, extracting features of gene sequences, transforming the gene into points in a higher dimensional space, analyzing the point distribution of the same sample in the higher dimensional space, determining a constructing network of a geometric solid covering the sample subspace and forecasting the gene transferring horizontally.

A long-grained rice classified controlling gene PHR1 and its use are disclosed. It is cloned from long-grained rice 93-11 and approved gene for controlling long-grained classification and named PHR1. Transfer-gene complementary examination proofs that PHR1 is gene for differentiating long-grained rice classification. It can obtain transfer-gene rice with various anti-insect abilities by inhibiting or excess expressing PHR1 to regulate plant resistance by gene engineering technology.

The invention discloses a method to screen and culture barley transfer-gene plant with non-antibiotic of agricillin dielectric conductance in plant transfer-gene technical domain, which comprises the following steps: leading reported gene into receptor barley cell through agricillin dielectric conductance; utilizing mannose to replace antibiotic as chosen agent; screening the reverting receptor cell; adjusting the genetic reforming process of barley through phosphomannose isomerase; further-screening transfer-gene barley with chlorophenol red colouration method and PCR method; getting the end product. The barley cultivating strain conversion rate can reach 12% with agricillin dielectric conductance and can reach 100% with chlorophenol red colouration method.

The invention refers to the ration checking method for transferring gene farm produce and food compound. The method includes folloiwng steps: the extraction of gene group DNA from transferring gene farm produce and food compound; checks the design of sign gene, special gene added ingredient and probe; ascertains the standard curve of ration checking sample; real-time fluorescence ratio polymerase chain reaction (PCR) checking, ration analysis.

The present invention provides methods for producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope, using neuraminidase (NA) derived from Gram-positive bacteria. The methods comprise the steps of culturing cells producing a viral vector in the presence of an NA from Gram-positive bacteria, and recovering the produced virus. The methods of this invention enable the production of high titer virus at high cost performance. Such a viral vector is capable of transferring genes at high efficiency into cells such as blood cells and hematopoietic cells, including hematopoietic stem cells, and mucous cells including mucoepithelial cells, those not amenable to gene transfer by conventional methods, and therefore should be useful as a vector for gene therapy.

The invention relates to a novel plant genetic transformation method. An ovary injection method is utilized to efficiently and directly introduce vector plasmid DNA dissolved in dimethyl sulfoxide solution into plants so as to obtain transgenic plants.