Method of establishing regeneration system and genetic transformation system of wild tomato solanum sities

Abstract

The invention relates to the field of genetic transformation, and specifically relates to a method of establishing a regeneration system and a genetic transformation system of wild tomato solanum sities. The method comprises following steps: (1) solanum sitie aseptic seedlings are bred; (2) three different explants, namely the root, the stem, and leaves of solanum sities, are induced to produce callus tissue and multiple shoot points in a callus-inducing medium (CIM); (3) the induced multiple shoots are individually separated, then put in a aseptic seedling medium, and the multiple shoots are induced to generate roots and turn into seedlings in the aseptic seedling medium. Wild tomato solanum sities instead of modern technology clutivated tomatoes which have strong genotype specificity are chosen as the research objects, which benefit researches in tomato genetic engineering and the process in tomato molecular breeding. The built corresponding systems of solanum sities are suitable for the three explants, and the transformation medium and the culture conditions for the three explants are completely the same, therefore related research cost is largely reduced. More important, the method has advantages in that: the regeneration and transformation efficiencies are high, the system is stable and the testing time is short.

Description

technical field [0001] The invention relates to the field of genetic transformation, in particular to a method for constructing a regeneration system and a genetic transformation system of wild tomato. Background technique [0002] Tomato is a worldwide economic crop of the family Solanaceae. Wild tomato Riki solanum is a wild relative of modern cultivated tomato. Its natural growth environment is special and its geographical distribution is narrow. It only exists in Chile and Peru in South America and is on the verge of extinction. However, its biological growth is huge and fast, especially its rich wild gene resources, which are valuable gene sources for modern tomato resistance, hybrid breeding and variety improvement. At present, domestic and foreign researches on Solanaceae plants, especially tomato, mostly focus on the conventional hybrid breeding of tomato, the interaction mechanism between tomato and pathogenic bacteria, the synthesis and metabolism of lycopene and t...

AI Technical Summary

Problems solved by technology

At present, domestic and foreign researches on Solanaceae plants, especially tomato, mostly focus on the conventional hybrid breeding of tomato, the interaction mechanism between tomato and pathogenic bacteria, the synthesis and metabolism of lycopene and tomatine, tomato heat resistance, salt tolerance and tomato development. In terms of molecular biology, etc., there have been no systematic reports on the regeneration and genetic transformation system of the wild relative of tomato, Solanum solani.

Images