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Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water

A phytoplankton and eutrophication technology, which is applied in the analysis and determination of chlorophyll a of phytoplankton in eutrophic water bodies, the analysis and determination of phytoplankton chlorophyll a in eutrophic water bodies, and the extraction field, which can solve the problems of incomplete extraction and operation. The process is complicated, toxic and other problems, to achieve the complete effect of extraction

Inactive Publication Date: 2010-10-13
TONGJI UNIV
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  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a new method for the analysis and determination of phytoplankton chlorophyll a in eutrophic water bodies, so as to make up for the incomplete extraction, toxicity, and operating process of traditional methods for detecting phytoplankton chlorophyll a in water bodies. Complicated and other defects, in order to achieve efficient, fast, accurate and environmentally friendly determination of the content of chlorophyll a contained in phytoplankton in water bodies

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  • Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water
  • Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water
  • Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water

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Embodiment 1

[0029] Embodiment 1: Determination of chlorophyll a of phytoplankton in a certain city river course in Shanghai

[0030] According to the traditional sampling method for detecting the chlorophyll of the phytoplankton in the water body, after collecting the water samples of a river course in Shanghai, use the hot ethanol method of the present invention and the traditional grinding method to measure the chlorophyll a of the phytoplankton in the water body according to the following steps.

[0031] (1) For the concentration of chlorophyll a of phytoplankton in the water body measured by hot ethanol method:

[0032] Take 200ml of water sample for suction filtration (using glass fiber filter membrane with diameter of 47mm and pore size of 0.22μm and mixed fiber filter membrane with diameter of 60mm and pore size of 0.45μm for suction filtration), put the filter membrane and the phytoplankton attached to it in Put it into a 10mL centrifuge tube with a stopper, and then put it in a -...

Embodiment 2

[0044] Embodiment 2: Determination of chlorophyll a of phytoplankton in a certain park in Shanghai

[0045] According to the traditional sampling method for detecting chlorophyll of phytoplankton in the water body, water samples of a park in Shanghai were collected, and the concentration of chlorophyll a of phytoplankton in the water body was measured using the hot ethanol method of the present invention and the traditional grinding method.

[0046] (1) For the concentration of chlorophyll a of phytoplankton in the water body measured by hot ethanol method:

[0047] Take 500ml water sample for suction filtration (using a glass fiber filter membrane with a diameter of 47mm and a pore size of 0.22μm), put the filter membrane after suction filtration and the phytoplankton attached to it into a 10mL centrifuge tube with stopper, and then put it into -18 Freeze in the refrigerator for 8 hours at ℃, take out the centrifuge tube with stopper and return to room temperature, add 10mL o...

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Abstract

An analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water is a new method for extracting, analyzing and measuring the chlorophyll a of phytoplankton in eutrophic water and relates to the technical field of restoration of water environment in the environmental engineering. The method comprises the following steps of: filtering and freezing a collected water sample and then adding hot ethanol; after the water bathing and the ultrasonic treatment, extracting the water sample for a plurality of hours in dark, centrifuging the water sample, taking the supernatant and measuring the content of the chlorophyll in a water body by using the spectrophotometry. The invention has the advantages of simple and quick extraction and measurement of the sample, capability of extracting the chlorophyll in the phytoplankton to the most extent, high degree of extraction, low toxicity, good accuracy, low limit of detection and wide range of detection concentration; the problems that the traditional grinding method has high loss degree of chlorophyll and low extraction efficiency and the acetone extraction is toxic to human bodies to a certain extent are solved; and the invention is suitable for rapidly extracting and accurately measuring the chlorophyll of phytoplankton in the eutrophic water.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering water environment restoration, in particular to a method for analyzing and measuring chlorophyll a of phytoplankton in eutrophic water bodies, which is a new method for extracting, analyzing and measuring chlorophyll a of phytoplankton in eutrophic water bodies . Background technique [0002] Green plants use chlorophyll for photosynthesis. Chlorophyll mainly includes chlorophyll a and chlorophyll b, which are synthetic natural low-molecular organic compounds. They are insoluble in water but soluble in organic solvents, such as ethanol, acetone, ether, and chloroform. Among them, chlorophyll a can directly reflect the growth of algae in the water body. If the content is high, there will be more algae, and if the content is low, there will be less algae. Excessive algae reduce the transparency and dissolved oxygen of the water body, affect the photosynthesis of organisms under th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/34
Inventor 周雪飞黄丽龚本涛张亚雷
Owner TONGJI UNIV
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