Method for detecting contents of chlorophyll a and carotinoid in crop laminas

A technology of carotene and chlorophyll, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of complex operation, many links, and long time consumption, and achieve the effect of simple equipment, easy operation, and fast speed

Inactive Publication Date: 2012-06-27
ZHEJIANG UNIV
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Problems solved by technology

[0006] The invention provides a method for detecting the content of chlorophyll a and carotenoids in the leaves of agricultural crops. The measurement result is stable, the operation is simple, the cost is low, the speed is fast, no chemical reagents and extraction are required, and the traditional method for measuring plant leaf pigments is time-consuming and time-consuming. Many links and complex operations

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  • Method for detecting contents of chlorophyll a and carotinoid in crop laminas
  • Method for detecting contents of chlorophyll a and carotinoid in crop laminas
  • Method for detecting contents of chlorophyll a and carotinoid in crop laminas

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the embodiments and accompanying drawings, but the present invention is not limited thereto.

[0025] Below take paddy rice leaf as example to illustrate the inventive method:

[0026] A method for detecting chlorophyll a and carotenoid content in leaves of crops, comprising the following steps:

[0027] (1) Rice is used as the crop to be tested, and the flag leaves of different rice varieties before maturity are used as the measurement object. A group of 12 leaves with different leaf colors are selected, and the entire leaf or 2 / 3 of the entire leaf is taken, and it is protected from light. , moisturizing, as a leaf sample;

[0028] (2) Use a scanner to scan the group of leaf samples taken respectively, and save them as different leaf image files in tiff format; then use computer image analysis software (Image J image processing software is used here) to analyze each The leaf image file is pr...

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Abstract

The invention discloses a method for detecting contents of chlorophyll a and carotinoid in crop laminas. The method comprises the following steps of: selecting a set of laminas with obviously different leaf colours, obtaining an image brightness value of the set of lamina samples through scanning and image analyzing and processing, and, with the help of the contents of the chlorophyll a and the carotinoid of the set of laminas accurately measured by using a spectrophotometry, respectively establishing a functional relation between the chlorophyll a and the image brightness value of the laminas and a functional relation between the carotinoid and the image brightness value of the laminas; and then, obtaining an image brightness value of a to-be-detected lamina through scanning and image analyzing and processing, and respectively calculating the contents of the chlorophyll a and the carotinoid in the to-be-detected lamina according to the functional relations. The method disclosed by the invention has the advantages of being simple in used equipment, simple and convenient for operation, low in cost and rapid in speed; chemical reagents and extraction are not required; detection is not influenced by time and an external environment; a measurement result is steady; and the method can be used for rapidly detecting the contents of the chlorophyll a and the carotinoid in the crop laminas anytime and anywhere.

Description

technical field [0001] The invention belongs to a method for detecting plant physiological indexes, in particular to a method for detecting the contents of chlorophyll a and carotenoids in crop leaves. Background technique [0002] Chlorophyll is the material basis for photosynthesis in plants, and its content is one of the important indicators in the study of plant physiology. The color of leaves has an inseparable relationship with its pigment content. People often use the change of leaf color as the basis for judging the growth state, physiological characteristics and nutritional status of plants. Therefore, it is of great significance to study and establish a method for the determination of leaf pigment content. [0003] The traditional method of analyzing plant leaf pigments is to first extract and then measure by spectrophotometry, which is based on the fact that chlorophyll a and chlorophyll b have maximum absorption at 645nm and 663nm, and the two absorption curves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
Inventor 崔海瑞王伟博舒庆尧
Owner ZHEJIANG UNIV
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