Method for determining content of saffron in medicinal composition

A measurement method, the technology of saffron, applied in the field of medicine, can solve the problems of unstable peak time, easy tailing, and many interference components, and achieve the effects of stable measurement results, enhanced elution ability, and reduced interference

Active Publication Date: 2014-01-22
JINHE TIBETAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Above-mentioned HPLC measures the saffron content determination result in the saffron medicinal material and the simple composition of prescription is satisfactory, and degree of separation is better, but for the pharmaceutic

Method used

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  • Method for determining content of saffron in medicinal composition
  • Method for determining content of saffron in medicinal composition
  • Method for determining content of saffron in medicinal composition

Examples

Experimental program
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Example Embodiment

[0031] Experimental example 1 The condition selection and method verification of the content determination of saffron in pharmaceutical composition by HPLC

[0032] Take Zuzhu Darcy as an example for condition selection.

[0033] 1. Instruments, reagents and test samples

[0034] Instrument: High performance liquid chromatograph: Hitachi L-7110 pump, Hitachi-7420 detector; Shimadzu AUW220D electronic balance.

[0035] Reference substance: crocin-I reference substance (batch number: 111588-200501), crocin-II reference substance (batch number: 100001-200504).

[0036] Sample: Zuozhu Dasi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) batch number: 20090501, 20090502, 20090503.

[0037] 2. Mobile phase selection

[0038] Using the mobile phase in the prior art, that is, methanol-water or acetonitrile-water system, the content of saffron in a pharmaceutical composition with complex ingredients was determined. As a result, the separation between the main peak of the sample and the i...

Example Embodiment

[0065] Example 1 A high performance liquid chromatography method for determining the content of safflower

[0066] Sample: Zuozhu Dasi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) batch number: 20090501, 20090502, 20090503.

[0067] A high performance liquid chromatography method for determining the content of the pharmaceutical composition Zuozhu Daxi Zhongxi Safflower, the steps are as follows:

[0068] Chromatographic conditions and system applicability: use octadecylsilane bonded silica as filler; use methanol-acetonitrile-0.05mol / L ammonium acetate as mobile phase, the volume ratio is 30:5:65; detection wavelength is 440nm; The number of theoretical plates should not be less than 4000 calculated based on the crocin-I peak;

[0069] Preparation of saffron control solution: accurately weigh crocin-I and crocin-II reference substance, add 60% methanol by volume to make 30μg crocin-I, 15μg crocin in 1ml -II mixed solution, ready;

[0070] Preparation of the test solution...

Example Embodiment

[0075] Example 2: A high performance liquid chromatography method for the content determination of safflower

[0076] Sample: Zuozhu Dasi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) batch number: 20090501, 20090502, 20090503.

[0077] A high performance liquid chromatography method for determining the content of the pharmaceutical composition Zuozhu Daxi Zhongxi Safflower, the steps are as follows:

[0078] Chromatographic conditions and system applicability: use octadecylsilane bonded silica as filler; use methanol-acetonitrile-0.05mol / L ammonium acetate as mobile phase, the volume ratio is 40:5:55; detection wavelength is 440nm; The number of theoretical plates should not be less than 4000 calculated based on the crocin-I peak;

[0079] Preparation of saffron control solution: accurately weigh crocin-I and crocin-II reference substance, add 60% methanol by volume to make 30μg crocin-I, 15μg crocin in 1ml -II mixed solution, ready;

[0080] Preparation of the test solut...

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Abstract

The invention discloses a method for determining the content of saffron in a medicinal composition, and aims to solve the problem that the content of saffron in a complex medicinal composition is difficult to determine. According to the method, methanol-acetonitrile-0.05mol/L ammonium acetate in a volume ratio of (30-45):5:(50-65) is adopted as a mobile phase, and a high performance liquid chromatography is adopted for determination; the mobile phase is a multi-solvent system, the mobile phase system is kept to be stable by adopting buffer salt, and the elution power is reinforced. The method provided by the invention is stable in determination result and good in linearity, the correlation coefficient, the resolution and the number of theoretical plates can meet the requirement of measurement standard, and a good detection result can be obtained on the saffron in a medicinal composition with a complex prescription.

Description

technical field [0001] The invention relates to a method for determining the content of a traditional Chinese medicine compound preparation, in particular to a method for determining the content of saffron in a pharmaceutical composition, and belongs to the technical field of medicine. Background technique [0002] Saffron, also known as saffron, saffron, and the stigma of Crocus sativus L., a plant of the family Iridaceae, is a famous and precious Chinese medicinal material with strong physiological activities, sedative, expectorant, and antispasmodic effects. As a precious medicinal material, saffron is used in many prescriptions, especially in ethnic medicines such as Tibetan medicine, such as Zhuzhu Daxi, Renqing Changjue, Ganchang Capsule, Zhenlong Xinnao Capsule, Twenty-five flavors of coral balls, etc. [0003] At present, there are many methods for determining the content of saffron, such as ultraviolet spectrophotometry, high-performance liquid chromatography, etc....

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 马宏伟赵延霞孙绪丁任松鹏魏永义
Owner JINHE TIBETAN MEDICINE
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