Method for determining content of saffron in medicinal composition
A measurement method, the technology of saffron, applied in the field of medicine, can solve the problems of unstable peak time, easy tailing, and many interference components, and achieve the effects of stable measurement results, enhanced elution ability, and reduced interference
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experiment example 1
[0031] Experimental example 1 Condition selection and methodological verification for the content determination of saffron in pharmaceutical composition by HPLC
[0032] Take Zuozhu Darcy as an example to carry out conditional selection.
[0033] 1. Instruments, reagents and test samples
[0034] Instrument: High performance liquid chromatography: Hitachi L-7110 pump, Hitachi-7420 detector; Shimadzu AUW220D electronic balance.
[0035] Reference substances: Crocin-I reference substance (batch number: 111588-200501), crocin-II reference substance (batch number: 100001-200504).
[0036] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.
[0037] 2. Mobile phase selection
[0038] Using the mobile phase in the prior art, i.e. methanol-water or acetonitrile-water system, to measure the content of saffron in the pharmaceutical composition with complex components, as a result, the separation R between the main p...
Embodiment 1
[0065] Example 1 A high-performance liquid chromatography method for content determination of saffron
[0066] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.
[0067] A high-performance liquid chromatography method for content determination of the pharmaceutical composition Zuzudaxi Chinese saffron, the steps are as follows:
[0068] Chromatographic conditions and system applicability: octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile-0.05mol / L ammonium acetate is used as mobile phase, and its volume ratio is 30:5:65; detection wavelength is 440nm; The number of theoretical plates calculated based on the crocin-I peak should not be less than 4000;
[0069] Preparation of saffron reference solution: Accurately weigh crocin-I and crocin-II reference substances, add 60% methanol by volume to make each 1ml contain 30 μg crocin-I and 15 μg saffron - the mixed solution of II, to get f...
Embodiment 2
[0075] Example 2: A high-performance liquid chromatography method for content determination of Zuzudaxi Chinese saffron
[0076] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.
[0077] A high-performance liquid chromatography method for content determination of the pharmaceutical composition Zuzudaxi Chinese saffron, the steps are as follows:
[0078] Chromatographic conditions and system applicability: octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile-0.05mol / L ammonium acetate is used as mobile phase, and its volume ratio is 40:5:55; detection wavelength is 440nm; The number of theoretical plates calculated based on the crocin-I peak should not be less than 4000;
[0079] Preparation of saffron reference solution: Accurately weigh crocin-I and crocin-II reference substances, add 60% methanol by volume to make each 1ml contain 30 μg crocin-I and 15 μg saffron - the mixed soluti...
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