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Clenbuterol matrix standard substance and preparation method thereof

A standard substance and solid substance technology, applied in the field of clenbuterol matrix standard substance and its preparation, can solve problems such as quality control and result calibration difficulties, and achieve good market application value, far-reaching social benefits, and health protection effects

Inactive Publication Date: 2012-11-28
SHANGHAI INST OF MEASUREMENT & TESTING TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome defects such as the matrix effect of the existing clenbuterol detection method, the quality control of the detection process and the difficulty in result calibration, and provide a clenbuterol matrix standard substance and its Preparation

Method used

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  • Clenbuterol matrix standard substance and preparation method thereof
  • Clenbuterol matrix standard substance and preparation method thereof
  • Clenbuterol matrix standard substance and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Preparation of Clenbuterol-based standard substance in pig urine freeze-dried powder

[0035] The first step, freeze-dried powder preparation

[0036] For the preparation process, see the attached figure 1 .

[0037] 1. Material preparation

[0038] About 2L of fresh pig urine samples were collected from healthy pigs at the Shanghai Nanhui Animal Health Supervision Institute, put them in an ice box and immediately transferred to the laboratory. Use quantitative analysis filter paper to filter pig urine at room temperature (20°C) to remove solid matter, collect the filtered pig urine sample into a 5L beaker, and add a clean magnet to it, stir with a magnetic stirrer at a stirring speed of 500rpm 30min to make it fully mixed.

[0039] The above mixed pig urine samples were tested by LC / MS / MS and ELISA methods, and the content of clenbuterol was less than 0.10ng / mL, which was used as the base urine sample and stored in a refrigerator at 4°C for later use.

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Abstract

The invention discloses a clenbuterol matrix standard substance and a preparation method thereof. The preparation method comprises the following steps of: uniformly mixing pig urine in which a solid substance is removed; then adding a clenbuterol standard substance to a quantity value level; uniformly mixing to obtain a mixed solution; and carrying out uniformity check on the mixed solution and then carrying out subpacking and freeze drying to obtain the clenbuterol matrix standard substance. The preparation method disclosed by the invention is scientific and reasonable and is strong in operability; the prepared matrix standard substance can be stored under the condition of -20DEGC and provides a quantity value tracing substance for detecting clenbuterol residue in daily pig urine; and according to the prepared matrix standard substance, the problem that different detection results are caused by matrix difference is solved and the monitoring of the clenbuterol residue in pig products is accurately ensured.

Description

technical field [0001] The invention relates to a clenbuterol matrix standard substance and a preparation method thereof. Background technique [0002] Clenbuterol, commonly known as "Clenbuterol", is a β-adrenergic receptor agonist, which can promote biological growth and significantly increase the lean meat rate of animals. It has been widely used as a growth-promoting feed additive (grams of hydrochloric acid in feed Determination of Renbuterol by HPLC method, Ying Yongfei, Wu Pinggu, etc., Feed Testing, 2006, 23(7): 54-56), but after a series of food poisoning incidents caused by eating animal sources containing "clenbuterol", Clenbuterol has been banned as a feed additive by many countries in the world, and requires that clenbuterol must not be detected in animal-derived foods (Ministry of Agriculture of the People's Republic of China, Maximum Residue Limits of Veterinary Drugs in Animal Food [Z], 1997, Agriculture and Animal Husbandry (1997) No. 7). In the breeding f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/42G01N33/558
Inventor 刘刚李兰英徐勤闻艳丽
Owner SHANGHAI INST OF MEASUREMENT & TESTING TECH
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