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Hydrobiontic algae chlorophyll measuring method

A determination method and chlorophyll technology, applied in algae products, botanical equipment and methods, applications, etc., can solve the problems of strong volatilization of reagents, large deviation of measurement results, long test time, etc., and achieve good reproducibility and reproducibility. Improved and stable measurement results

Inactive Publication Date: 2005-06-29
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY +1
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Problems solved by technology

Some water quality monitoring departments in my country also use this method for determination, but the reagents used in this method are highly volatile and toxic, pollute the working environment, and endanger the health of testers. Moreover, the filter membrane required by this method depends on imports, and the test time is long and the test cost is high. high
At present, there is no national standard method for determining the chlorophyll content of algae in water in my country. The domestically recommended method has the disadvantages of large deviation of measurement results, unstable measurement results, poor reproducibility, and pollution of the working environment.

Method used

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Effect test

Embodiment

[0058] 1. Equipment and reagents

[0059] (1) Vacuum pump, the vacuum degree is not greater than 0.05MPa;

[0060] (2) All-glass vacuum filtration device;

[0061] (3) Magnetic or agate mortars;

[0062] (4) Medical centrifuge, rotating speed 3500-4000r / min, load capacity 500-675g;

[0063] (5) Centrifuge tube, 15mL, with graduated scale and screw cap;

[0064] (6) Spectrophotometer, the minimum scale is 0.5-2nm;

[0065] (7) Cuvette, optical path 1 ~ 3cm;

[0066] (8) Acetate cellulose membrane, with a diameter of 50 mm and a pore size of 0.8 to 1.0 μm;

[0067] (9) 90% ethanol aqueous solution (volume ratio);

[0068] (10) 1% MgCO 3 Suspension: add 1.0g MgCO to 100ml distilled water 3 powder;

[0069] 2. Measuring procedure

[0070] (1) Sample preparation. Take a water sample of 500mL at a certain depth under the water surface. If the algae content is high, the water volume can be reduced accordingly. Because the content of algae varies greatly at different water...

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Abstract

The invention discloses a hydrobiontic algae chlorophyll measuring method which consists of, using spectrophotometry method to measure the algae chlorophyll content in the water, filtering subaqueous algae with cellulose acetate microporous filtering film, carrying out interception to the algae cellulose acetate through immersion with 90% ethanol, extracting the chlorophyll in algae cell into ethanol, centrifuging the tobacco extract to obtain the clarified solution, using 90% ethanol as reference, determining absorbancy of the tobacco extract at 630, 647, 664 and 750 nm with a spectrophotometer, calculating content of chlorophyll a, b, c in the water sample by utilizing the measured data.

Description

technical field [0001] The invention relates to a method for measuring chlorophyll of aquatic algae, in particular to a method for measuring chlorophyll of aquatic algae by spectrophotometry, which is suitable for measuring chlorophyll a, chlorophyll b and chlorophyll c of algae in water. Background technique [0002] Water eutrophication and algae blooms lead to deterioration of water quality. In order to evaluate the degree of eutrophication of water body, the content of algae should be measured. Algae are green plankton that contain chlorophyll for photosynthesis. Algae chlorophyll includes chlorophyll a, chlorophyll b and chlorophyll c. All algae contain chlorophyll a, which accounts for about 1-2% of the dry weight of the organism, and some algae also contain chlorophyll b and c. Chlorophyll a accounts for the vast majority of chlorophyll and is the energy transfer center in the entire photosynthesis process. Therefore, the use of chlorophyll, especially chlorophyll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H13/00
Inventor 黄廷林丛海兵何文杰
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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