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Breeding method of Spirulina alga species

A technology of spirulina and algae species, applied in the direction of bacteria, etc., can solve problems such as difficulty in adapting to environmental factors and inability to apply large-scale production, and achieve the effects of good quality, increased yield, and improved product quality

Inactive Publication Date: 2015-09-09
JIANGXI ZHONGZAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, open or semi-closed, runway-type circulation culture ponds are widely adopted at home and abroad. Many environmental factors, especially temperature and light intensity, are difficult to control artificially. Although it showed excellent production performance in the small test, it cannot be applied to large-scale production because it is difficult to adapt to large changes in environmental factors after entering the production pool

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The processing steps of the breeding method of the spirulina algae species of the present practical example are:

[0021] A. Take 5L of algae culture solution with spirulina algae, and filter it with 150 mesh silk cloth to remove individual small algae;

[0022] B. Wash the filtered algae with pH 7.0 phosphate buffer, and then suspend them in phosphate buffer; after NTG filtration and sterilization, add them to the algae liquid at a final concentration of 100 μg / mL, and treat them in a water bath at 37°C in the dark 25min; centrifuge the mutagenized algae solution and wash with sterile pH7.0 phosphate buffer;

[0023] C. Add water to dilute 5 times, and use a capillary tube under the microscope to select a single spirulina with a large algae body and more than 13 spiral circles; put it in different test tubes and add culture medium to cultivate;

[0024] D. During the cultivation process, the optical density method is used to continuously measure the growth of algae an...

Embodiment 2

[0029] The processing steps of the breeding method of the spirulina algae species of the present practical example are:

[0030] A. Take 5L of algae culture solution with spirulina algae, and filter it with 200 mesh silk cloth to remove individual small algae;

[0031] B. Wash the filtered algae with pH 7.0 phosphate buffer, and then suspend them in phosphate buffer; after NTG filtration and sterilization, add them to the algae liquid at a final concentration of 100 μg / mL, and treat them in a water bath at 37°C in the dark 25min; centrifuge the mutagenized algae solution and wash with sterile pH7.0 phosphate buffer;

[0032] C. Add water to dilute 5 times, and use a capillary tube to select a single spirulina with a large algae body and more than 12 spiral circles under a microscope; put them in different test tubes and add culture medium for cultivation.

[0033] D. During the cultivation process, the optical density method is used to continuously measure the growth of algae...

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Abstract

The invention discloses a breeding method of Spirulina alga species, which comprises the following steps: 1. selecting 5L of alga culture solution, filtering with a silk fabric to remove smaller algae; 2. carrying out mutagenesis with a phosphate buffer solution; 3. selecting single Spirulina with larger body and more spiral rings; culturing in a test tube, and calculating the growth rate; 4. culturing the Spirulina elements subjected to amplification culture in a 40001*12 DEG C lighting incubator; and 5. selecting the Spirulina element with the highest growth rate to obtain the mutant line subjected to mutagenesis screening, purification and cloning. The method changes the coarse breeding method of screening the alga species by the filter screen into capillary selected breeding; and the unicell screening is utilized to remove bad Spirulina individuals to obtain high purification, and the mutagenesis passivation is performed to obtain the high-quality high-growth-rate Spirulina alga species. The alga species purification and culture conditions are continuously optimized, so that the Spirulina yield is enhanced to 30-50%, and the product quality is further improved.

Description

technical field [0001] The invention relates to the technical field of spirulina, in particular to a method for breeding spirulina species. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral shape. At present, a huge spirulina industry has been formed on the basis of human population research, and it is used in food, biomedicine and health care, feed, fine chemicals and other fields. [0003] Spirulina is the most widely used variety in commercial breeding production and development at home and abroad. It is worth pointing out that many experimental studies and long-term production practices have shown that there are many strains of Spirulina species, and they have requirements for environmental factors such as temperature, light quality, light intensity, and salinity, pH, and nutrient content of the culture solution. Significantly different from adaptability. At present, open or se...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 郑祖凤郑训艺
Owner JIANGXI ZHONGZAO BIOTECH
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