8 results about "Spirochaete" patented technology

A chewable tablet a first probiotic strain of bacteria that inhibits Streptococcus mutans; a second probiotic strain of bacteria that inhibits one or more of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (i.e., the red complex); a remineralizing agent such as hydroxyapatite with a micron-level particle size; and a sugar alcohol such as xylitol.

The invention relates to an aptamer which binds to the CspZ protein present on the surface of bacteria of the genus Borrelia, and to the use thereof in an in vitro method for diagnosing an infection with a bacterium of the genus Borrelia in a subject.

The present invention discloses a variant D-allulose 3-epimerase (DAEase) that is distinguished from a wild-type DAEase derived from Spirochaetales bacterium (spDAEase) by at least one amino acid sequence variation comprising substitution of an 5 amino acid residue corresponding to position 69, 119, 138, 175, 203, 208, 229, 240, or 265 of SEQ ID NO. 1; or a variant DAEase that is distinguished from a wild-type DAEase derived from Clostridium cellulolyticum (ccDAEase) by an amino acid sequence variation comprising substitution of an amino acid at position 46 and / or a truncation of C-terminal end of in SEQ ID NO. 2.

The invention provides a blood microorganism-based autism prediction model and application thereof, the prediction model takes blood microorganisms as markers, and the detection rate or relative abundance of the blood microorganisms is reduced; the risk that the blood sample suffers from autism is predicted by increasing the detection rate of blood microorganisms II or enhancing the relative abundance; the first blood microorganisms comprise ochrobactrum, pseudoalteromonas sp.3J6, rhodococcus equi, parabacteroides dieldrii, trichoderma harzianum, enterobacter hormaechei, wolbachia, yersinia enterocolitica, treponema pallidum and Elizabeth mosquito-repellent bacteria; the blood microorganisms II comprise Klebsiella compacticola and bdellovibrio bacteriovorus. According to the method, the model is constructed based on the blood metagenome data of 1946 standard autism four families, the advantages of blood microorganisms are brought into full play, disease signals are sensitively and accurately captured, the method can be applied to clinical autism prediction, and early risk assessment and layering of diseases are achieved.

The invention belongs to the technical field of biology, and discloses a method for determining a multivalent antigen sequence of an anti-periodontitis red complex, which comprises the following steps: firstly, screening out key virulence factor antigens (FimA-II, RgpA, Msp and BspA) from three core pathogenic bacteria of the red complex: porphyromonas gingivalis, treponema denticola and fusisterone; then, independently cloning each antigen gene to a pUC57 cloning vector, constructing a single antigen recombinant plasmid, and avoiding intergene interference by designing a specific enzyme cutting site; and finally, through plasmid transformation, positive clone screening and double enzyme digestion verification, the plasmid quality is ensured. The obtained single antigen plasmid can be flexibly combined and used, is beneficial to subsequent multivalent vaccine development, and has the advantages of wide antigen coverage, high expression reliability, flexible construction strategy, easiness in industrialization and the like.

Molecular probes for the diagnosis of Lyme borreliosis The invention relates to an aptamer which binds to the CspZ protein present on the surface of bacteria of the genus Borrelia and their use in an in vitro method for diagnosing an infection by a bacterium of the genus Borrelia in a subject.

The application provides an autism prediction model based on blood microorganisms and application thereof. The prediction model takes blood microorganisms as markers, and predicts the risk of autism of a blood sample through the decrease of a blood microorganism one detection rate or relative abundance, or the increase of a blood microorganism two detection rate or relative abundance. The blood microorganism one comprises Xanthobacteraceae bacterium, Pseudomonas sp.3J6, Rhodococcus equi, Parabacteroides distasonis, Xylella fastidiosa, Enterorhabdus hofmannii, Wolbachia, Yersinia enterocolitica, Borrelia parkeri and Elizabethkingia sp. The blood microorganism two comprises Klebsiella michiganensis and Bdellovibrio sp. The application constructs a model based on blood metagenomic data of 1946 standard four-generation families of autism, fully develops the advantages of blood microorganisms, sensitively and accurately realizes the capture of disease signals, and can be applied to the prediction of autism in the clinic, and realizes the early risk assessment and stratification of diseases.