4 results about "Spirochaete" patented technology

The invention relates to an aptamer which binds to the CspZ protein present on the surface of bacteria of the genus Borrelia, and to the use thereof in an in vitro method for diagnosing an infection with a bacterium of the genus Borrelia in a subject.

The invention belongs to the technical field of biology, and discloses a method for determining a multivalent antigen sequence of an anti-periodontitis red complex, which comprises the following steps: firstly, screening out key virulence factor antigens (FimA-II, RgpA, Msp and BspA) from three core pathogenic bacteria of the red complex: porphyromonas gingivalis, treponema denticola and fusisterone; then, independently cloning each antigen gene to a pUC57 cloning vector, constructing a single antigen recombinant plasmid, and avoiding intergene interference by designing a specific enzyme cutting site; and finally, through plasmid transformation, positive clone screening and double enzyme digestion verification, the plasmid quality is ensured. The obtained single antigen plasmid can be flexibly combined and used, is beneficial to subsequent multivalent vaccine development, and has the advantages of wide antigen coverage, high expression reliability, flexible construction strategy, easiness in industrialization and the like.

Molecular probes for the diagnosis of Lyme borreliosis The invention relates to an aptamer which binds to the CspZ protein present on the surface of bacteria of the genus Borrelia and their use in an in vitro method for diagnosing an infection by a bacterium of the genus Borrelia in a subject.

The application provides an autism prediction model based on blood microorganisms and application thereof. The prediction model takes blood microorganisms as markers, and predicts the risk of autism of a blood sample through the decrease of a blood microorganism one detection rate or relative abundance, or the increase of a blood microorganism two detection rate or relative abundance. The blood microorganism one comprises Xanthobacteraceae bacterium, Pseudomonas sp.3J6, Rhodococcus equi, Parabacteroides distasonis, Xylella fastidiosa, Enterorhabdus hofmannii, Wolbachia, Yersinia enterocolitica, Borrelia parkeri and Elizabethkingia sp. The blood microorganism two comprises Klebsiella michiganensis and Bdellovibrio sp. The application constructs a model based on blood metagenomic data of 1946 standard four-generation families of autism, fully develops the advantages of blood microorganisms, sensitively and accurately realizes the capture of disease signals, and can be applied to the prediction of autism in the clinic, and realizes the early risk assessment and stratification of diseases.