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Method for culturing spirulina by using livestock and poultry excrement wastewater

A technology of livestock and poultry excrement and spirulina, which is applied in the field of culturing spirulina by utilizing livestock and poultry waste waste water, can solve the problem of high cost of spirulina cultivation, and achieve the effects of reducing cultivation land, high quality and high cultivation efficiency

Active Publication Date: 2014-01-01
深圳市华胜隆科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the cultivation of spirulina is carried out in natural lakes or fresh water or seawater. It is still necessary to add the nutrients needed for the growth of spirulina, or to treat the culture medium to achieve the conditions suitable for the growth of spirulina, which will lead to the cultivation of spirulina. high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Pre-treatment of pig excrement wastewater:

[0040] First, filter the excrement of livestock and poultry and its washing water to remove the coarse residue, and dry the coarse residue as an organic fertilizer; then use HCl and NaOH to adjust the pH value of the filtrate to 8.6, and then pass through ozone to sterilize the filtrate ; The resulting liquid is the culture medium of spirulina.

[0041] (2) Domestication of Spirulina:

[0042] The spirulina algae are inoculated into seawater for acclimation culture, and the seawater used is used; in the acclimatization step, the temperature is controlled at 30° C., and the pH is maintained at 8.0.

[0043] (3) Cultivation of Spirulina:

[0044] Spirulina in the logarithmic growth phase in step (2) was inoculated into the culture medium obtained in step (1) at a ratio of 1:3, and cultured in a U-shaped track. During this process, the pH was maintained at 7.2, The light was maintained at 20000LX, the temperature was main...

Embodiment 2

[0050] (1) Pre-treatment of pig excrement wastewater:

[0051] The first is to filter the excrement of livestock and poultry and its flushing water, remove the coarse residue, and dry the coarse residue as organic fertilizer; then carry out anaerobic fermentation on the filtrate for 2 days to achieve the purpose of deodorization, and collect the generated biogas; then use HCl and NaOH to adjust the pH value of the filtrate to 9.0, and then pass through ozone to sterilize the filtrate; the obtained liquid is the culture liquid of spirulina.

[0052] (2) Domestication of Spirulina:

[0053] The spirulina algae are inoculated into seawater for domestication and cultivation, and the practical salinity of the seawater used is less than 35; in the domestication step, the temperature is controlled at 35° C., and the pH is maintained at 9.0.

[0054] (3) Cultivation of Spirulina:

[0055] The spirulina in the logarithmic growth phase in step (2) was inoculated into the culture mediu...

Embodiment 3

[0061] (1) Pre-treatment of pig excrement wastewater:

[0062] The first is to filter the excrement of livestock and poultry and its flushing water to remove the coarse residue, dry the coarse residue and use it as an organic fertilizer; then carry out anaerobic fermentation on the filtrate for 3 days to achieve the purpose of deodorization and collect the generated biogas; then use HCl and NaOH to adjust the pH value of the filtrate to 9.5, and then pass through ozone to sterilize the filtrate; the obtained liquid is the culture liquid of spirulina.

[0063] (2) Domestication of Spirulina:

[0064] The spirulina algae are inoculated into seawater for domestication and cultivation, and the practical salinity of the seawater used is less than 35; in the domestication step, the temperature is controlled at 37° C., and the pH is maintained at 10.0.

[0065] (3) Cultivation of Spirulina:

[0066] Spirulina in the logarithmic growth phase in step (2) was inoculated into the cultu...

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Abstract

The invention provides a method for culturing spirulina by using livestock and poultry excrement wastewater, belonging to the fields of sewage treatment and algal culture. The method comprises the following steps: (1) pretreatment of livestock and poultry excrement wastewater: filtering, and sterilizing the filtrate to obtain a culture solution; (2) domestication of spirulina: inoculating an alga strain into fresh water or sea water, and performing domestication culture; (3) culture of spirulina: inoculating the spirulina in a logarithmic growth phase of the step (2) into the culture solution of the step (1), and culturing; (4) collection of spirulina: filtering the spirulina cultured in the step (3), cleaning, collecting the spirulina, and then drying the spirulina; and (5) filtrate recovery. The method provided by the invention solves the problems that the livestock and poultry excrement pollutes the environment and the spirulina culture cost is high. The method can realize the triple effects of protecting the environment, lowering the spirulina culture cost and producing nutrient substances required by human beings.

Description

technical field [0001] The invention relates to the fields of sewage treatment and algae cultivation, in particular to a method for cultivating spirulina by utilizing livestock and poultry excrement waste water. Background technique [0002] With the development of the economy, there are more and more large-scale livestock and poultry farms, so the amount of livestock and poultry excrement that is intensively processed is very large. If the treatment method is not appropriate, it will easily lead to environmental pollution. The treatment methods of livestock and poultry excrement in traditional livestock and poultry farms are: (1) direct discharge; (2) discharge into ponds for fish farming; (3) solid-liquid separation, dry the solid to make compost, and discharge the liquid directly; ( 4) Fermentation is used to produce biogas, which is used as fuel, and the fermented liquid is discharged directly. The traditional method can also turn waste into wealth to a certain extent, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F11/04C05F3/00C12R1/89
CPCY02E50/343Y02A40/20Y02E50/30Y02W30/40
Inventor 高荣盛屠爱华
Owner 深圳市华胜隆科技有限公司
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