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Method for preparing phycocyanin of sea water

A technology of phycocyanin and seawater, applied in the direction of single-cell algae, algae/moss peptide, peptide source, etc., can solve the problems of low production and application value, long process route, high cost, etc., achieve high production and application value, and improve extraction Efficiency and the effect of reducing extraction costs

Inactive Publication Date: 2003-08-27
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation method of phycocyanin reported at present all adopts the spirulina obtained from cultivating the fresh water medium with low salinity as raw material, and there is no report of useful seawater or artificial seawater as the medium; in addition, the preparation of existing phycocyanin Methods, see "Chinese Science (C)" Volume 30, the 5th phase PP449-454 reported algae cells through repeated freezing and thawing broken cells, frozen high-speed centrifugation method to carry out solid-liquid separation, ammonium sulfate salting out, 0- 4°C dialysis, chromatographic column, multiple gradient elution, eluent salting out with ammonium sulfate, 0-4°C dialysis, low temperature freeze-drying, etc. Although high purity can be achieved, the process route is long and most processes need to be It is carried out at low temperature and the extraction period is about one week, so the cost is high, the efficiency is low, and the production and application value is not high

Method used

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Examples

Experimental program
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Effect test

example 1

[0018] [Example 1] Spirulina is inoculated in natural seawater culture medium with a salinity of 60‰, cultivated in a large outdoor pool, harvested algae mud, washed with seawater, dried at 30-60°C to obtain 210 grams of dried algae, and added 5 liters 0.005M phosphate buffer solution (containing 1% NaCl, 50ppm sodium benzoate, and 100ppm glycerin), soaked at 25-35°C, stirred and extracted for 12 hours, centrifuged to remove residue, and obtained 4.3 liters of supernatant, concentrated to 0.5 liters by ultrafiltration, Adjust the buffer solution concentration to 0.075M (pH6.7, containing 0.2MNaCl), add 0.075M phosphate buffer solution (pH6.7, containing 0.2MNaCl) balanced hydroxyapatite, after stirring, centrifuge to remove hydroxyapatite, the obtained Ammonium sulfate was added to the supernatant to reach a saturation of 60%, and a precipitate was formed. The precipitate was collected by centrifugation, ultrafiltered, and 0.005M phosphate buffer solution (pH6.7, containing 0.2...

example 2

[0019] [Example 2] Inoculate Spirulina in artificial seawater culture medium with a salinity of 35‰, expand the cultivation to 10 square meters outdoors, harvest the algae mud, rinse with fresh water, dry in the shade to obtain 1.8 kg of dry algae, add 20 liters of 0.04M Phosphate buffer solution (containing 5% NaCl, 50ppm sodium benzoate, 100ppm glycerol), soaked at 10-25°C, stirred and extracted for 24 hours, centrifuged to remove residue, obtained 20 liters of supernatant, concentrated to 5 liters by ultrafiltration, added About 200ml of hydroxyapatite balanced by 0.05M phosphate buffer solution (pH6.7, containing 0.2MNaCl), after stirring, centrifuged to remove hydroxyapatite, and the obtained supernatant was added with ammonium sulfate to reach a saturation of 60%, forming a precipitate. The precipitate was collected by centrifugation, desalted by ultrafiltration and concentrated, 5 grams of glucose and 1 gram of NaCl were added, and vacuum freeze-dried at ultra-low temper...

example 3

[0020] [Example 3] Inoculate Spirulina in natural seawater culture medium with a salinity of 42‰, expand the cultivation outdoors to 100 square meters, harvest algae mud, rinse with fresh water, and dry at 30-60°C to obtain 6 kg of dry algae , add 80 liters of 0.04M phosphate buffer solution (containing 5% NaCl, 50ppm sodium benzoate, 100ppm glycerol), soak at 10-15°C, stir and extract for 24 hours, centrifuge to remove residue, and obtain 55 liters of supernatant, which is ultrafiltered Concentrate to 20 liters, add about one liter of hydroxyapatite balanced by 0.04M phosphate buffer solution (pH6.7, containing 0.2MNaCl), after stirring, centrifuge to remove hydroxyapatite, and add ammonium sulfate to the obtained supernatant to reach saturation The concentration was 60%, a precipitate was formed, and the precipitate was collected by centrifugation, desalted by ultrafiltration and concentrated, 30 grams of glucose and 5 grams of NaCl were added, and spray-dried to obtain 625 g...

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Abstract

The invention refers to a making method of algae blue protein by using seawater spirulina as raw material. The current making method has long technical course, long cycle of extraction, high cost, low efficiency, low application value and mostly is made at low temperature. The invention can largely and at low cost extract higher-purity algae glue protein, is applied to research and development of natural pigment, health food and new sea medicament.

Description

technical field [0001] The invention relates to a preparation method for extracting phycocyanin from seawater spirulina. technical background [0002] Seawater spirulina (Spirulina, seawater strain) is a seawater culture strain of Cyanophyta, Homogoneles, Oscillatoniaceae, Spirulina or Arthrospira. Take the lead in realizing industrialized farming in the world. By adapting to seawater stress conditions, the content, stability and biological activity of phycocyanin in seawater spirulina can be significantly improved. [0003] The preparation method of phycocyanin reported at present all adopts the spirulina obtained from cultivating the fresh water medium with low salinity as raw material, and there is no report of useful seawater or artificial seawater as the medium; in addition, the preparation of existing phycocyanin Methods, see "Chinese Science (C)" Volume 30, the 5th phase PP449-454 reported algae cells through repeated freezing and thawing broken cells, frozen high-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C12N1/12C12P21/00
Inventor 向文洲何慧林坚士董俊德何妙娟吴伯堂曾呈奎
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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