Method for extracting polysaccharide of spirulina platensis

The technology of a spirulina polysaccharide and an extraction method is applied in the extraction field of spirulina polysaccharide, which can solve the problems of less impurities, high biological activity and high purity, and achieve the effects of improving efficiency and simple operation.

Active Publication Date: 2013-05-01
YUNNAN PHYTOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no process route that can guarantee less impurities, high purity,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 100kg of spirulina, add 4 times the amount of 95% ethanol, reflux extraction once, each time for 1 hour, pour off the ethanol, dry the solid, add 4 times the amount of water, extract once, extract 2 hours, the extraction temperature is Centrifuge the extract at 15,000 r / min at 60°C, filter the supernatant through a 1.5 μm microporous membrane, add the filtrate to an ultrafiltration machine, and select a filter material with a molecular weight of 0.5 million for ultrafiltration until ultrafiltration When there is no liquid at the machine outlet, the concentrated solution in the ultrafiltration machine is collected and dried to obtain 17 kg of water-extracted spirulina crude polysaccharide, with a total sugar content of 12% and a molecular weight of more than 5,000.

Embodiment 2

[0034] Take 100kg of spirulina, add 10 times the amount of 95% ethanol, reflux extraction 3 times, each time for 3 hours, pour off the ethanol, dry the solid, add 20 times the amount of water, extract 5 times, each time 10 hours, the extraction temperature At 100°C, combine the extracts, centrifuge at 9000 r / min, combine the supernatants, filter through a 1.2 μm microporous membrane, add the filtrate to an ultrafiltration machine, and select a filter material with a molecular weight of 10,000 for ultrafiltration , when no liquid comes out from the outlet of the ultrafilter, collect the concentrated liquid in the ultrafilter, and then use a filter material with a molecular weight of more than 50,000 to perform ultrafiltration on the concentrated liquid, and when there is no liquid at the outlet of the ultrafilter, collect the concentrated liquid from the ultrafilter Concentrate and effluent within. Dry them separately to obtain 8 kg of water-extracted spirulina crude polysaccha...

Embodiment 3

[0036] Take 100kg of spirulina, add 10 times the amount of 95% ethanol, reflux extraction 3 times, each time for 3 hours, pour off the ethanol, dry the solid, add 20 times the amount of water, extract 5 times, each time 10 hours, the extraction temperature at 100°C, combine the extracts, centrifuge at 15,000 r / min, combine the supernatants, and heat to reduce the volume to 5 times the amount of Spirulina fed. Add 50% ethanol to the concentrated solution until the alcohol content reaches 40%, stir well, let it stand for 12 hours, suck up the supernatant, collect the precipitate, and add water to dissolve it. Centrifuge at a speed of 15000r / min, take the supernatant, combine, filter through a 1.5μm microporous membrane, add the filtrate to the ultrafiltration machine, select a filter material with a molecular weight of 0.5 million for ultrafiltration, and reach the outlet of the ultrafiltration machine. When the liquid comes out, the concentrated liquid in the ultrafiltration ma...

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PUM

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Abstract

The invention discloses a method for extracting polysaccharide of spirulina platensis. The method comprises the steps that spirulina platensis is subjected to an extraction process to form an extracting solution, the extracting solution is filtered through a microfiltration membrane and is subjected to ultrafilteration, concentration and drying, then more than one type of crude polysaccharide of aqueous extract spirulina platensis is obtained, the content of total sugar is larger than or equal to 10%, and the molecular weight is more than 5,000; or the extracting solution is concentrated and decontaminated, a processed solution is centrifuged under the rotating speed lower than or equal to 15,000 r/min, filtered through the microfiltration membrane and subjected to ultrafilteration by a filter material with the molecular weight in a range from 5,000 to 200,000, when no effluent flows out from an outlet of an ultrafilter, the effluent of the ultrafilter and a concentration solution in the ultrafilter are collected to be dried, then more than one type of polysaccharide of aqueous extract spirulina platensis is fromed, the content of total sugar is larger than or equal to 40%, and the molecular weight is in a range from 5,000 to 200,000. According to the method for extracting polysaccharide of spirulina platensis, reasonable processing measures are selected at different stages of a proces route according to different requirements for product purity, and then high purity olysaccharide with the scheduled molecular weight specification can be formed without detection; and moreover, polysaccharide of spirulina platensis with a pluraity of specifications can be formed simultaneosuly. Accordingly, the method has the advanatags which are impossible to be obtained by other traditional methods.

Description

technical field [0001] The invention relates to an extraction method for simultaneously obtaining spirulina polysaccharides in various molecular weight ranges. The prepared polysaccharides of different specifications can be used in medicine, food, health products and cosmetics. Background technique [0002] Spirulina is an ancient single-celled algae in the ocean, which contains a large amount of amino acids, cyanobacteria, carotenoids, γ-linolenic acid, inositol and other nutrients. Spirulina polysaccharide is a class of important natural bioactive substances extracted and separated from Spirulina algae and Spirulina culture fluid, which has the functions of promoting cell growth, improving immunity, anti-tumor, anti-radiation, and anti-aging. This disease can also significantly enhance the dual effect of immunity of the body. [0003] There are a lot of proteins in spirulina, which are polymer substances like polysaccharides, and some water-soluble proteins and polysacch...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 梅艳李俊
Owner YUNNAN PHYTOPHARML
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