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Composite culture medium for bifidobacterium fermentation and preparation method thereof

A technology for bifidobacteria and culture medium, which is applied to the field of compound medium for bifidobacteria fermentation and its preparation, and can solve the problem that bifidobacteria have harsh nutritional and environmental conditions, poor pH tolerance of TPY medium, quality and production conditions It is difficult to control and other problems, to achieve the effect of saving energy consumption and equipment cost, good economic benefits and low cost

Active Publication Date: 2007-10-24
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present TPY culture medium has the following problems: the first is that the formula is more complicated, the quality and production conditions are not easy to control, there are many types of raw materials and the price is high, and it is not suitable for large-scale fermentation; the second is that too many inorganic salts need to be added, which is harmful to the human body. The third is that bifidobacteria have harsh requirements on nutrition and environmental conditions, poor tolerance to low nitrogen source and low pH of TPY medium, shortened survival time, and low fermentation biomass. meet production requirements

Method used

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  • Composite culture medium for bifidobacterium fermentation and preparation method thereof
  • Composite culture medium for bifidobacterium fermentation and preparation method thereof
  • Composite culture medium for bifidobacterium fermentation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] The composition of the compound culture medium for Bifidobacterium infantis fermentation is:

[0108] Standard Spirulina Powder (LVT) 800g

[0109] Stachyose 600g

[0110] 5% pork liver extract 400mL

[0111] Distilled water to make up 10000mL

[0112] Preparation of compound medium for fermentation of Bifidobacterium infantis:

[0113] (1) Preparation of standard spirulina micropowder (LVT):

[0114] a. Select commercially available food-grade or feed-grade spirulina powder with an average particle size of 150-250 μm.

[0115] b. After weighing the spirulina powder, use a 60-mesh sieve to remove impurities.

[0116] c. The spirulina powder taken by sieving is frozen at a low temperature to the critical point of fragmentation (glass point), and then air-jet milling is carried out by the jet-jet milling method until the soluble spirulina powder with an average particle size of 60-80 μm is obtained , dried, packaged and sealed for later use.

[0117] (2) Preparatio...

Embodiment 2

[0121] The composition of the compound culture medium for Bifidobacterium adolescentis fermentation is:

[0122] Standard Spirulina Powder 800g

[0123] Stachyose 500g

[0124] 5% pig liver extract 500mL

[0125] Distilled water to make up 10000mL

[0126] Preparation of compound culture medium for Bifidobacterium adolescentis fermentation:

[0127] (1) Preparation of standard spirulina micropowder (LVT):

[0128] a. Select commercially available food-grade or feed-grade spirulina powder with an average particle size of 150-250 μm.

[0129] b. After weighing the spirulina powder, use a 60-mesh sieve to remove impurities.

[0130] c. The spirulina powder taken by sieving is frozen at a low temperature to the critical point of fragmentation (glass point), and then air-jet milling is carried out by the jet-jet milling method until the soluble spirulina powder with an average particle size of 60-80 μm is obtained , dried, packaged and sealed for later use.

[0131] (2) Prep...

Embodiment 3

[0138] The composition of the compound culture medium for Bifidobacterium longum fermentation is:

[0139] Standard Spirulina Powder 800g

[0140] Stachyose 400g

[0141] 5% pork liver extract 600mL

[0142] Distilled water to make up 10000mL

[0143] Preparation of compound medium for fermentation of Bifidobacterium longum:

[0144] (1) Preparation of standard spirulina micropowder (LVT):

[0145] a. Select commercially available food-grade or feed-grade spirulina powder with an average particle size of 150-250 μm.

[0146] b. After weighing the spirulina powder, use a 60-mesh sieve to remove impurities.

[0147] c. The spirulina powder taken by sieving is frozen at a low temperature to the critical point of fragmentation (glass point), and then air-jet milling is carried out by the jet-jet milling method until the soluble spirulina powder with an average particle size of 60-80 μm is obtained , dried, packaged and sealed for later use.

[0148] (2) Preparation of stach...

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Abstract

The invention discloses a composite culture medium for fermentation of bifidobacteria and the manufacturing method, which comprises the following parts: 50-150g spirulina powder, 40-80g stachyose, 30-100ml 5% pork liver infusion, 1000ml distilled water. The effect of the invention is that: the infant bifidobacteria, adolescent bifidobacteria and long bifidobacteria grow well in the culture medium; the maximum increment reaches 1010cfu / ml in the best fermentation condition; the submerged culture effect is the best of the classic bifidobacteria selective culture medium, TPY culture medium and improved TPY culture medium. The composite culture medium provides a simple formulation, a low cost, a simple manufacturing process, which can be used to the research, exploitation, production of bifidobacteria, provides the important meanings of utilizing and developing spirulina and stachyose resource.

Description

technical field [0001] The invention belongs to the technical field of production of medicines, food and food additives, veterinary medicine, biological feed and feed additives, and in particular relates to a compound culture medium for bifidobacterium fermentation and a preparation method thereof. Background technique [0002] Bifidobacterium (Bifidobacterium) is the most important physiological bacteria in the human body and the core dominant flora in the normal flora of the gastrointestinal tract. Long-term and a large number of studies have shown that in the whole life process of humans and domesticated animals, the multiple physiological regulatory functions of Bifidobacteria play a vital role in maintaining their healthy state; in other words, almost all non-healthy states of the body , are directly or indirectly related to the imbalance of the number and function of bifidobacteria. Because of this, since the late 1970s, the development and industrialization of bifido...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 吴力克王宇明
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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