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Extraction and determination method of crude polysaccharide in ganoderma spirulina

A determination method and technology of crude polysaccharides, which are applied in the preparation of test samples, material analysis by observing the influence of chemical indicators, and analysis by causing materials to undergo chemical reactions, etc., can solve the problem of lack of reliable detection methods and determination The accuracy of the data is not high, and there are not many problems, so as to achieve the effect of simplifying the steps of extraction and determination, high sensitivity and high accuracy

Active Publication Date: 2010-09-22
NINGBO YUFANGTANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Cracked ganoderma lucidum spore powder and spirulina powder have been proven to have the function of enhancing immunity as water-soluble crude polysaccharides, but mature and reliable extraction of water-soluble crude polysaccharides There are not many methods for polysaccharides, and there are almost no corresponding reliable detection methods
Especially because there are many kinds of interfering components in Ganoderma lucidum spirulina health products, the accuracy of the measurement data of many determination methods is not high, and the steps of extraction and determination methods are also relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Precisely weigh 0.9552g of Ganoderma lucidum spirulina tablet fine powder, add 30g of 80% ethanol solution, oscillate and stir evenly, ultrasonically extract for 40min, centrifuge and discard the solution. Add 30 g of 80% ethanol solution to the precipitation, oscillate and stir evenly, ultrasonically extract for 40 min, and centrifuge to discard the solution. Add the precipitate to 30ml of purified water and extract in a water bath at 100°C for 3 hours, filter, transfer the filtrate to a 100ml volumetric flask, wash the residue 4 times, transfer the washed solution to the volumetric flask to constant volume, shake well, and use it as a sample solution.

[0032] Accurately weigh 0.1080g of glucose standard product dried to constant weight, add distilled water to dissolve and constant volume in a 1000ml volumetric flask to make a 0.108mg / ml control solution, and take the glucose solution 0, 0.2ml, 0.4ml, 0.6ml, 0.8ml, and 1.0ml were respectively placed in 20ml stoppered ...

Embodiment 2

[0038] Precisely weigh 0.5016g of Ganoderma lucidum spirulina tablet fine powder, add 15g of 80% ethanol aqueous solution, oscillate and stir evenly, ultrasonically extract for 20min, centrifuge and discard the solution. Add 15 g of 80% ethanol aqueous solution to the precipitate, oscillate and stir evenly, ultrasonically extract for 20 min, and centrifuge to discard the solution. Add 50ml of purified water to the precipitate and extract in a water bath at 100°C for 1 hour, filter, transfer the filtrate to a 100ml volumetric flask, wash the residue twice, transfer the washing solution to the volumetric flask to constant volume, shake well, and use it as a sample solution. Draw 1ml sample solution in the stoppered test tube of 20ml, prepare with measuring standard solution method, measure absorbance A=0.356 at 490nm place with ultraviolet spectrophotometer, bring sample solution absorbance into equation and promptly obtain the content of crude polysaccharide in sample solution, ...

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Abstract

The invention discloses an extraction and determination method of crude polysaccharide in ganoderma spirulina, which comprises the following steps: weighing ganoderma spirulina samples accurately and putting the samples into a great capacity centrifuge tube, adding ethanol aqueous solution and mixing evenly, carrying out ultrasonic extraction and centrifugation, and discarding solution; adding the ethanol aqueous solution to the obtained sediment, vibrating and mixing evenly, carrying out ultrasonic extraction and centrifugation, precipitating, then moving to a round bottomed flask after adding water, after extraction in boiling water bath, filtering, bringing filtrate to volume, and determining the content of the crude polysaccharide by phenol-sulphuric acid method by taking glucose or dextran standardized products solution as a reference material. The method has simple steps, is accurate, has high sensitivity and is especially appropriate for the extraction and determination of the crude polysaccharide in the ganoderma spirulina which is health food and does not contain disruptors, dextrin, starch and the like.

Description

technical field [0001] The invention relates to a method for extracting and measuring crude polysaccharides in Ganoderma lucidum spirulina health food, in particular to a method for extracting and measuring crude polysaccharides in Ganoderma lucidum spirulina tablets. Background technique [0002] Ganoderma lucidum spores are extremely small spores ejected from the cap during the mature growth period of Ganoderma lucidum. Ganoderma lucidum is called top grade by "The Classic" and is known as "immortal grass". Ganoderma lucidum spore powder is the reproductive cells of Ganoderma lucidum, which has all the genetically active substances of Ganoderma lucidum, and its nutritional value and pharmacological effects are much higher than that of Ganoderma lucidum itself. Ganoderma lucidum spores contain proteins, amino acids, glycopeptides, vitamins, carotene, sterols, triterpenes, alkaloids, fatty acids, lactones and inorganic ions. Among them, crude polysaccharides can resist tumo...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N1/34
Inventor 黄金宝贾福怀邢晓伟
Owner NINGBO YUFANGTANG BIOTECH
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