Preparation method of novel decellularized tendon or ligament bracket

Abstract

The invention relates to a preparation method of a decellularized tendon or ligament bracket, and aims at providing a decellularized tendon or ligament collagen fiber material with good biomechanical characteristic and good biocompatibility. The preparation method comprises the following steps: acquiring animal tendon or ligament with the adventitia or synovial tissue removed, wherein during the whole decellularization process, a mixed protecting liquid containing a ligament or tendon support structure protective agent is adopted; pre-separating cells in the ligament or tendon tissue by adopting a high hydrostatic pressure technology; digesting the residual cell nuclei by adopting a compound nuclease method; removing loose broken cell sheets by adopting a detergent; and carrying out rinsing by adopting the mixed protecting liquid. Through the efficient physical means, the adoption of small amount of enzyme and the detergent removing means, the cellular constituent in the ligament or tendon can be sufficiently removed, and the protecting liquid is adopted for the whole-process protection, so that on the basis of guaranteeing the complete removal of the cell nuclei, while the immunogenicity of the tendon or ligament tissue is reduced to the maximum, the three-dimensional structure and arrangement polarity of the normal collagenous fibers of the tendon or ligament tissue are reserved.

Description

technical field

[0001] The invention relates to the field of preparation of bioengineering medical materials, in particular to a preparation method of decellularized tendon / ligament scaffold. Background technique

[0002] Poor healing ability after repair of ligament and tendon injury is a common clinical problem, and the use of substitutes for ligament and tendon repair and reconstruction is urgently needed clinically. Currently tendon and ligament substitutes mainly include autografts, allografts or xenografts, and synthetic material grafts, but all have their unavoidable shortcomings. Although autologous ligament transplantation is the most effective method of repair and reconstruction, it cannot be widely used clinically due to the limited range and quantity of materials obtained. Allogeneic or heterogeneous tendon has strong antigenicity, and the inevitable postoperative immune rejection is its biggest shortcoming. Artificial synthetic materials such as carbon fiber, ...