Polycaprolactone/methylacryloyl elastin nanofiber composite membrane and preparation method and application thereof

A technology of methacrylylating elasticity and polycaprolactone, which is applied in the field of medical materials for tendon injury repair, can solve the problems that biological materials cannot meet clinical requirements, and achieve tissue engineering repair, avoid cytotoxicity, and be easy to batch the effect of

Active Publication Date: 2020-11-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings and deficiencies that the single-performance biomaterials existing in the prior art are difficult to meet the clinical requirements, the primary purpose of the present invention is to provide a preparation of polycaprolactone / methacrylated elastin nanofiber composite membrane method

Method used

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  • Polycaprolactone/methylacryloyl elastin nanofiber composite membrane and preparation method and application thereof
  • Polycaprolactone/methylacryloyl elastin nanofiber composite membrane and preparation method and application thereof
  • Polycaprolactone/methylacryloyl elastin nanofiber composite membrane and preparation method and application thereof

Examples

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Embodiment 1

[0036] A preparation method of polycaprolactone / methacryloyl elastin nanofiber composite film includes the following steps:

[0037] (1) Weigh 10g of soluble elastin and add it to 100mL of PBS solution, stir under a constant temperature water bath at 50℃ until the elastin is completely dissolved; add 20ml of methacrylic anhydride to 10w through a syringe pump at a rate of 0.5ml / min / v% elastin solution, and then placed at 50°C and stirred for 3h; then add 400mL of 50°C PBS solution to dilute the reaction solution of elastin and methacrylic anhydride, stir for 30min and terminate the reaction; add the diluted solution Put it into a dialysis bag with MW=1000 and dialyzed in deionized water at 40°C for 12 days. During this period, the fluid was changed every 8h to remove unreacted methacrylic anhydride and by-products; after 12 days, take it out and pass the dialyzed solution through Filter on qualitative filter paper and collect the filtrate; pre-freeze the collected filtrate and f...

Embodiment 2

[0041] A preparation method of polycaprolactone / methacryloyl elastin nanofiber composite film includes the following steps:

[0042] (1) Weigh 10g of soluble elastin and add it to 100mL of PBS solution, stir under a constant temperature water bath at 50℃ until the elastin is completely dissolved; add 20ml of methacrylic anhydride to 10w through a syringe pump at a rate of 0.5ml / min / v% elastin solution, and then placed at 50°C and stirred for 3h; then add 400mL of 50°C PBS solution to dilute the reaction solution of elastin and methacrylic anhydride, stir for 30min and terminate the reaction; add the diluted solution Put it into a dialysis bag with MW=1000 and dialyzed in deionized water at 40°C for 12 days. During this period, the fluid was changed every 8h to remove unreacted methacrylic anhydride and by-products; after 12 days, take it out and pass the dialyzed solution through Filter on qualitative filter paper and collect the filtrate; pre-freeze the collected filtrate and f...

Embodiment 3

[0046] A preparation method of polycaprolactone / methacryloyl elastin nanofiber composite film includes the following steps:

[0047] (1) Weigh 10g of soluble elastin and add it to 100mL of PBS solution, stir under a constant temperature water bath at 50℃ until the elastin is completely dissolved; add 20ml of methacrylic anhydride to 10w through a syringe pump at a rate of 0.5ml / min / v% elastin solution, and then placed at 50°C and stirred for 3h; then add 400mL of 50°C PBS solution to dilute the reaction solution of elastin and methacrylic anhydride, stir for 30min and terminate the reaction; add the diluted solution Put it into a dialysis bag with MW=1000 and dialyzed in deionized water at 40°C for 12 days. During this period, the fluid was changed every 8h to remove unreacted methacrylic anhydride and by-products; after 12 days, take it out and pass the dialyzed solution through Filter on qualitative filter paper and collect the filtrate; pre-freeze the collected filtrate and f...

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Abstract

The invention belongs to the field of medical materials for tendon injury repair and discloses a polycaprolactone/methylacryloyl elastin nanofiber composite membrane and a preparation method and application thereof. The preparation method specifically comprises the following steps of grafting methacrylic anhydride to elastin to obtain the methylacryloyl elastin; then mixing polycaprolactone with the methylacryloyl elastin uniformly to prepare a spinning solution; carrying out electrostatic spinning by using the spinning solution to obtain a composite membrane; and soaking the composite membrane by using a photoinitiator solution, carrying out photo-crosslinking under ultraviolet light and then carrying out washing and drying for many times to obtain the polycaprolactone/methylacryloyl elastin nanofiber composite membrane. The preparation method is easy to operate and relatively low in production cost, and the obtained composite membrane is good in mechanical property and biocompatibility and can be used for tendon tissue engineering and tissue repair.

Description

Technical field [0001] The invention belongs to the field of tendon injury repairing medical materials, and specifically relates to a polycaprolactone / methacrylylated elastin nanofiber composite film and a preparation method and application thereof. Background technique [0002] Tendon injuries caused by various traumas and diseases are very common clinically. Statistics show that there are at least 30 million tendon injuries worldwide every year. Unlike bone tissue, tendon healing cannot form a homogenous tissue, returning to an undamaged state. Conversely, the formation of fibrous scars causes the tendon stress to be weaker than that of the native tendon, and the resulting lack of strength may increase the risk of re-injury and other complications. Clinical repair methods for tendon defects mainly include autologous tendon transplantation, allogeneic tendon transplantation, xenogeneic tendon transplantation and prosthetic material transplantation. However, these tendon regen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/728D04H1/4382D01F8/02D01F8/14D06M10/00C08H1/00A61L27/22A61L27/18A61L27/50A61L27/56D06M101/32D06M101/02
CPCD04H1/728D04H1/4382D01F8/02D01F8/14D06M10/001C08H1/00A61L27/227A61L27/18A61L27/50A61L27/56D06M2101/32D06M2101/02A61L2430/10C08L89/00C08L67/04
Inventor 杜昶吕玲美吴晶晶
Owner SOUTH CHINA UNIV OF TECH
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