Two channel-based multi-spectrum fluorescent imaging microscopic system and method

Abstract

The invention is applicable to the field of optics, biomedicine, life science and the like, and provides a two channel-based multi-spectrum fluorescent imaging microscopic system and method, wherein the two channel-based multi-spectrum fluorescent imaging microscopic system comprises a picosecond pulse laser device, a fluorescent excitation and collection light path, a microscopic objective lens, a light beam lens, a double-ICCD detector, and a control and processing module. The invention further discloses a method for performing multi-spectrum imaging by utilizing the two channel-based multi-spectrum fluorescent imaging microscopic system. According to the two channel-based multi-spectrum fluorescent imaging microscopic system and method, the limitation of the existing fluorescent microscope and a fluorescent life imaging microscopic system only can acquire single wavelength fluorescent signal with one-time detection can be effectively solved, the simultaneous acquisition of the multi-spectrum fluorescent strength and fluorescent light image aiming at the dynamic process of fluorescent intensity-related detection limited in biomedicine and life science can be performed, so that the research and application ranges of biophotonics can be extended.

Description

technical field [0001] The invention relates to the field of optical imaging technology and biomedicine, in particular to a dual-channel-based multispectral fluorescence imaging microscope system and method. Background technique [0002] In recent years, with the progress of biomedical research, the means of obtaining biophotonics information by using fluorescence lifetime microscopy imaging has been gradually adopted. Combined with fluorescence intensity imaging, the fluorescence lifetime imaging method can be used for the detection of cellular microenvironment and imaging of fluorophores with overlapping spectra. Specifically, it can complete the transfer of fluorescence resonance capabilities, quantitative monitoring of intracellular physical and chemical environments such as oxygen ion concentration, calcium ion, and pH value, etc. . [0003] At present, fluorescence microscopy imaging systems that can detect fluorescence lifetimes mainly include: single-photon fluoresc...

AI Technical Summary

Problems solved by technology

[0004] In the existing technology, the fluorescence microscopy system mostly monitors the fluorophore of a single wavelength, or uses a spectrometer combined with a detector to image and fuse the wavelength of interest; Bands of interest, or detection of bands of interest one by one, such as dynamic monitoring of living cells, FRET process, etc., the existing system cannot simultaneously acquire multiple bands of interest in the entire monitoring process and perform fusion processing on the image information

Images