A Cell Membrane Imaging Reagent Based on Fluorescent Nanoparticles

Abstract

The invention discloses a fluorescent nanoparticle-based cell membrane imaging reagent, which consists of component A and component B; the component A is an amino-bearing polymer whose side chain contains a biotin molecular unit and a hydrophobic anchor unit; The component B is fluorescent nanoparticles grafted with avidin molecules. Based on fluorescent nanoparticles and multi-site anchoring technology, the reagent has good biocompatibility and excellent imaging effect. tracer mark.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a cell membrane fluorescent imaging reagent based on fluorescent nanoparticles. Background technique [0002] The cell membrane not only serves as the boundary of the cell structurally, providing a relatively stable internal environment for the cell, but also plays a vital role in the process of material transportation, energy conversion and information transmission between the cell and the external environment. As a powerful research method, cell membrane imaging technology uses fluorescent dyes to label and trace the cell membrane, revealing many important biological processes including pinocytosis, exocytosis, cell division and apoptosis. [0003] However, some traditional lipophilic dyes not only endocytose quickly, but the imaging time is short; and these small organic molecule dyes are easy to be quenched (such as FITC and other organic fluorescent molecules will disappear afte...

AI Technical Summary

Problems solved by technology

However, most of the current fluorescent nanoparticles are used for the overall labeling of cells and the tracking of specific organelles, but not for the specific imaging of cell membranes. The main reason is that fluorescent nanoparticles themselves are easily endocytized by cells, thus losing cell membrane labels. specificity

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