Preparation and application for magnetic nanometer microspheres used for magnetic relaxation targeted determination of cancer cells

A magnetic nanometer and cancer cell technology, which can be used in the preparation of in vivo experiments, the determination/inspection of microorganisms, and measurement devices, etc. It can solve the problems of high biological toxicity, accelerated magnetic relaxation measurement, commercial and medical applications, and magnetic saturation strength. Low-level problems, to achieve the effects of low biological toxicity, broad medical application prospects, and high sensitivity

Inactive Publication Date: 2020-06-02
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the above-mentioned magnetic materials can be developed, it will solve the disadvantages of low magnetic saturation intensity and high biological toxicity of traditional magnetic nanomaterials, and will greatly accelerate the commercial and medical applications of magnetic relaxometry, providing new paths and opportunities for early diagnosis of cancer. new ideas

Method used

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  • Preparation and application for magnetic nanometer microspheres used for magnetic relaxation targeted determination of cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) One-step synthesis method to prepare superparamagnetic Fe 3 o 4 @PEG-COOH nanoparticles

[0035] Dissolve iron acetylacetonate and dicarboxypolyethylene glycol in 2-pyrrolidone. Nitrogen gas is fed into the solution to remove oxygen, and then the reaction is carried out for 60-90 minutes under the conditions of a rotating speed of 300-400 rpm and a temperature of 200-300°C. After the reaction was completed, nitrogen was continuously fed until the solution was cooled to room temperature, and then acetone-ether solution was added to the solution to obtain a black sticky precipitate. The black viscous precipitate was dissolved in deionized water and dialyzed for a period of time to remove the precipitate with molecular weight less than 12000. Then, the precipitate was repeatedly settled by acetone-ether solution and dried in vacuum to obtain superparamagnetic Fe 3 o 4 @PEG-COOH nanoparticles.

[0036] (2) Preparation of Fe by carbodiimide coupling method 3 o 4 ...

Embodiment 2

[0044] The steps of Example 2 of the present invention are the same as Example 1, the difference is that the composition ratio of the substance in the implementation steps is different, specifically as follows:

[0045] In the step (1), the mass ratio of dicarboxypolyethylene glycol to iron acetylacetonate is 5.5:1.

[0046] The dialysis time of the step (1) is 2 days.

[0047] Described step (2) cancer cell antibody, superparamagnetic Fe 3 o 4 The mass ratio of @PEG-COOH nanoparticles, N-hydroxysulfosuccinimide and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride is 20:4:1 :1

[0048] The volume ratio of acetone and ether in the step (2) acetone-ether solution is 1.3:1.

Embodiment 3

[0050] The steps of Example 3 of the present invention are the same as Example 1, the difference is that the composition ratio of the substance in the implementation steps is different, specifically as follows:

[0051] In the step (1), the mass ratio of dicarboxypolyethylene glycol to iron acetylacetonate is 6:1.

[0052] The dialysis time of the step (1) is 3 days.

[0053] Described step (2) cancer cell antibody, superparamagnetic Fe 3 o 4 The mass ratio of @PEG-COOH nanoparticles, N-hydroxysulfosuccinimide and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride is 25:6:1 :1

[0054] The volume ratio of acetone and ether in the step (2) acetone-ether solution is 1.5:1.

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Abstract

The invention discloses a preparation and an application for magnetic nanometer microspheres used for magnetic relaxation targeted determination of cancer cells. According to the invention, superparamagnetic Fe3O4@PEG-COOH nanoparticles are prepared by using ferric acetylacetonate and dicarboxyl polyethylene glycol as raw materials through a one-step synthesis method; and the magnetic nanometer microspheres Fe3O4@PEG-COOH are prepared by using the superparamagnetic Fe3O4@PEG-COOH nanoparticles, N-hydroxysulfosuccinimide and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride as raw materials through a carbodiimide coupling method. According to the invention, the antibody protein on the surface of a magnetic nanometer microsphere can be combined with a receptor on a cancer cell membrane, so specific recognition is realized. Through measurement of the magnetic relaxation time of the magnetic nanometer microspheres under a pulsed magnetic field through an atomic magnetometer, accurate detection of cancer cells is achieved; high detection speed, high detection precision and low detection limit are realized; a new idea and a new path are provided for early diagnosis of cancers;and wide medical application prospect is obtained.

Description

technical field [0001] The invention belongs to the field of nanomaterial manufacturing technology and biomedicine, and relates to a preparation method and application of magnetic nanometer microspheres, in particular to a preparation method and application of magnetic nanometer microspheres for magnetic relaxation targeting detection of cancer cells. Background technique [0002] With the rapid development of social economy, the discharge of a large amount of pollutants has caused serious environmental pollution and ecological resource damage, which has seriously threatened the living environment and life and health of human beings, leading to a continuous increase in the incidence of cancer. According to the report of the International Cancer Research Center (IARC), there were 12.66 million new cancer cases and 7.56 million deaths worldwide in 2008, accounting for 13% of all deaths. In the next few decades, the incidence of cancer will continue to increase rapidly. It is e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/16A61K49/12C12Q1/02
CPCA61K49/1875A61K49/186A61K49/16A61K49/126G01N33/5005
Inventor 王晨宇梁梓萱黄智淼
Owner HANGZHOU DIANZI UNIV
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