Method for synchronously detecting polycyclic aromatic hydrocarbons and derivatives thereof in blood and application thereof

Abstract

The invention discloses a method for synchronously detecting polycyclic aromatic hydrocarbons and derivatives thereof in blood and application thereof, and the method comprises the following steps: selecting related polycyclic aromatic hydrocarbons and derivatives thereof in blood in the same blood sample as target detection substances, and carrying out synchronous extraction, detection and analysis; to be specific, the method comprises the following steps: S1, establishing a PAHs and SPAHs relationship analysis mathematical model, screening SPAHs having relatively strong correlation with PAHs, and taking the screened SPAHs and PAHs as target detection objects; S2, extracting a target detection object; S3, purifying and separating the sample; and S4, carrying out sample concentration and detection: performing analyzing on a machine to obtain content data of multiple components of detected target objects PAHs and SPAHs. According to the application of the method, detected content data of PAHs and SPAHs in a blood sample and the relation are applied to evaluation of exposure characteristics of two kinds of pollutants commonly existing in the environment in people or individuals, the detection effect and specificity are improved, and the application range of detection data is expanded.

Description

technical field [0001] The invention belongs to the technical field of organic pollutant detection and human exposure risk assessment, and in particular relates to a simultaneous detection method and application of polycyclic aromatic hydrocarbons and their derivatives in blood. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants ubiquitous in the environment, and their sources are mainly divided into natural sources and anthropogenic sources. Man-made sources include incomplete combustion of organic substances such as coal, oil, and wood; natural sources mainly include fires caused by forests, eruptions from volcanic activities, etc. Studies have proved that PAHs have "three-cause" effects (carcinogenic, teratogenic, and mutagenic) on the human body. PAHs can be stable in the environment for a long time, have long-distance migration ability, and can enter the human body through various channels such as the respiratory tra...

AI Technical Summary

Problems solved by technology

However, due to the complex and diverse pathways of human exposure to PAHs and SPAHs, and at the same time affected by individual differences, it is difficult to extrapolate to the actual exposure dose in the human body, and it is difficult to accurately reflect the internal exposure of the human body. The direct detection of PAHs and SPAHs concentrations in the blood can avoid the influence of individual differences and exposure routes, and directly reflect the internal exposure of the human body.

[0004] At present, most of the detection methods for PAHs and PAH derivatives such as nitroPAHs are to detect PAHs and nitroPAHs alone, and most of them are based on samples in air, water, soil and other media. Detecting substances, such as Chinese invention patent document CN 111007192A, discloses a detection method of nitro-PAHs and PAHs in soil, which adopts high-performance liquid chromatography-fluorescence detector combination to simultaneously measure 3 kinds of PAHs and 1 kind of nitro PAHs, but it must purify and derivatize the sample, and the relationship between PAHs and nitro PAHs has not been studied; Chinese invention patent document CN106885852A discloses a The preparation of the dual-functional monolithic column of polycyclic aromatic hydrocarbons and the high-performance liquid chromatography method must also be treated in the method of monolithic column reduction-in-tube solid phase microextraction-high performance liquid chromatography-laser-induced fluorescence detection. The test sample is reduced, and then the separated reduction product sample is detected using a laser-induced fluorescence detector

Obviously, the above two methods have the disadvantages of cumbersome sample processing, detection and analysis steps, long time consumption, and large solvent consumption. At the same time, the impurities in the sample matrix are less, and it cannot be applied to blood and other impurities. complex sample handling

[0005] Since polycyclic aromatic hydrocarbons and polycyclic aromatic hydrocarbon derivatives belong to two types of substances with large differences in polarity, and the concentration of polycyclic aromatic hydrocarbon derivatives in the environment is low, the treatment of polycyclic aromatic hydrocarbons and polycyclic aromatic hydrocarbon derivatives at the same time, There are certain technical difficulties. At present, there are no reports on the simultaneous detection of PAHs and their derivatives SPAHs in the same blood sample, and reports on the analysis of two types of pollutants in the same blood sample.

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