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Application of honey to preparation of reagent for long-term dynamic near-infrared targeting imaging of inflamed parts

A targeted imaging and near-infrared technology, applied in echo/ultrasound imaging agents, nuclear magnetic resonance/magnetic resonance imaging contrast agents, preparations for in vivo tests, etc., can solve the real-time, dynamic, long-term schedule and other issues

Active Publication Date: 2015-06-24
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports of real-time, dynamic, long-term, near-infrared imaging agents for inflammation-related diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The application of honey in the dynamic long-term near-infrared targeted imaging reagent of the inflammatory site, the steps are:

[0027] 1) Construct a wound on the lower abdomen of Drosophila, induce an inflammation model, and set it as an experimental model group; set up a normal control group and a blank group at the same time.

[0028] 2) Inject 0.05ml of aseptic honey, propolis or royal jelly with a concentration of 30g / L into the experimental model group and the normal control group through a microinjector; the blank group is not treated.

[0029]3) During the period of 1 to 6 hours, through non-invasive real-time dynamic high-sensitivity rapid fluorescent tracing and detection, observe the transport of imaging reagents in Drosophila and enrich them in inflammatory lesions; the specific operations are:

[0030] Using a macro-zoom fluorescence microscope to image, select different time points such as 1 hour, 2 hours, and 6 hours, and put the fruit flies in CO 2 ...

Embodiment 2

[0034] 1) Create wounds on the neck or head of C57 mice, or induce inflammatory models such as cerebral apoplexy, and divide them into experimental model group and control model group; set up normal control group and blank control group at the same time.

[0035] 2) 0.3ml sterile concentration of 30g / L of honey, propolis or royal jelly or a mixture of any two reagent solutions, injected into the normal control group and experimental model group mice; control model group and blank Groups are not processed.

[0036] 3) During the period of 1 to 72 hours, through non-invasive real-time dynamic high-sensitivity rapid fluorescent tracing and monitoring, observe the circulation of the targeted reagent in the mouse body and enrich the inflammatory lesion; the specific steps are:

[0037] Use the in vivo fluorescence imager to image, select different time points such as 1 hour, 4 hours, 8 hours, 12 hours, 24 hours, 48 ​​hours, etc., and then anesthetize the mice with 5% isoflurane gas...

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PUM

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Abstract

The invention discloses application of honey to preparation of a reagent for the long-term dynamic near-infrared targeting imaging of inflamed parts. The reagent is honey, propolis or royal jelly with excellent biocompatibility, or the reagent is the mixture of honey and propolis or royal jelly or the mixture of propolis and royal jelly. The reagent can be used for imaging and marking related inflamed parts specifically, accurately and rapidly under the in-situ biological positioning action and tracing and monitoring related inflamed parts dynamically, sensitively and rapidly for a long time in a non-invading manner. In addition, the reagent has no biological side and toxic effects and can be used for examining conveniently. The reagent can be used for realizing the real-time long-term dynamic near-infrared targeting imaging and related model imaging of the inflamed parts and has wide medical application prospect.

Description

technical field [0001] The present invention relates to the application of honey in dynamic long-term near-infrared targeted imaging reagents at inflammatory sites. These reagents are composed of honey, propolis and royal jelly with excellent biocompatibility, and any one or any two of them are composed in any proportion The mixture of NIR imaging and its related multimodal imaging can realize non-invasive real-time dynamic long-term high-sensitivity rapid tracking and Monitoring, and no biological toxicity and side effects, the detection method is simple and easy. Background technique [0002] Inflammation refers to the defense response of living tissue with vascular system to damaging factors. It is what people usually call "inflammation". It is a very common and important pathological process, and it is a defense reaction of the body to stimulation, which is manifested as redness, swelling, heat, and pain. Vascular responses are central to the inflammatory process. In ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/04A61K49/06A61K49/22
Inventor 王雪梅来兰梅陈芸赵春秋李晓琦姜晖
Owner SOUTHEAST UNIV
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