The invention discloses a preparation method of a platinum nanocluster with uricase-like activity. Chloroplatinic acid is used as a reactant metal precursor, protamine is used as a modifier to stabilize and induce platinum nanoparticles to form a multistage cluster structure. The prepared platinum nanocluster has uricase-like activity, and the activity of the platinum nanocluster is comparable with that of natural uricase; the preparation method is simple, easy to operate, one-step in synthesis and easy for batch production; reaction conditions are mild, only normal temperature and normal pressure are needed, safety, no pollution and environmental friendliness are achieved, equipment investment is small, cost is low, and reproducibility is good; the used protein is high in safety and goodin thermal stability, and has quite high nutrition and functionality; the obtained product has the characteristics of no toxicity, good biocompatibility, simple purification and storage, good stability, high catalytic activity and the like, and has an important value in treatment of human hyperuricemia related diseases (such as gout).