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Gold/platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and application of fluorescence probe in aureomycin detection

A polyethyleneimine and bimetallic nanotechnology, applied in the field of fluorescent probes, can solve the problems of difficult operation, complicated methods and processes, etc.

Inactive Publication Date: 2017-09-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported methods and processes for the synthesis of Au / PtNCs are usually complex, inconvenient, and time-consuming.

Method used

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  • Gold/platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and application of fluorescence probe in aureomycin detection
  • Gold/platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and application of fluorescence probe in aureomycin detection
  • Gold/platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and application of fluorescence probe in aureomycin detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.25 μmol H 2 PtCl 6 ·6H 2 O and 1.25 μmol HAuCl 4 ·3H 2 O was dissolved in 10 mL of ultrapure water containing 25 μmol GSH, and after stirring for 20 minutes, 2.5 μmol PEI was added, and after 2 hours, 2.5, 12.5, 25, and 50 μmol AA were added respectively, so that the molar ratio of AA to (Pt+Au) was 1 :1, 5:1, 10:1 and 20:1. Then, the pH value of the mixed system was adjusted to 5 with 1.0 M HCl solution. Afterwards, the mixture was put into a 50mL autoclave, and subjected to hydrothermal reaction at 90°C for 60min to obtain polyethyleneimine-protected gold / platinum bimetallic nanoclusters (Au / PtNCs) with different AA amounts (moles). .

[0026] The results show that: when the molar ratio of AA to (Pt+Au) is 10:1, that is, when the AA in the reaction solution is 25 μmol. The fluorescence intensity of Au / PtNCs is the highest.

Embodiment 2

[0028] 2.25, 1.25, 0.25 μmol H 2 PtCl 6 ·6H 2 O and 0.25, 1.25, 2.25 μmol HAuCl 4 ·3H 2 O (the molar ratio of Pt to Au is 9:1, 1:1 and 1:9, respectively) was dissolved in 10mL ultrapure water containing 25μmol GSH, after stirring for 20min, 2.5μmol PEI was added, and after 2h, 25μmol AA was added . Then, the pH value of the mixed system was adjusted to 5 with 1.0 M HCl solution. Afterwards, the mixture was put into a 50mL autoclave and hydrothermally reacted at 90°C for 60min to obtain polyethylenimine-protected gold / platinum bimetallic nanoclusters (Au / PtNCs) under different molar ratios of Pt to Au.

[0029] The results show that when the molar ratio of Pt to Au is 1:1, the H in the reaction solution 2 PtCl 6 ·6H 2 O and HAuCl 4 ·3H 2 When O is 1.25 μmol and 1.25 μmol respectively. The fluorescence intensity of Au / PtNCs is the highest.

Embodiment 3

[0031] 1.25 μmol H 2 PtCl 6 ·6H 2 O and 1.25 μmol HAuCl 4 ·3H 2 O was dissolved in 10 mL of ultrapure water containing 2.5, 12.5, 25, and 50 μmol GSH respectively (the raw material molar ratios of GSH to Au were 1:1, 5:1, 10:1, and 20:1), and after stirring for 20 min, Then 2.5 μmol PEI was added, and after 2 h, 25 μmol AA was added. Then, the pH value of the mixed system was adjusted to 5 with 1.0 M HCl solution. Afterwards, the mixture was put into a 50mL autoclave, and hydrothermally reacted at 90°C for 60min to obtain polyethylenimine-protected gold / platinum bimetallic nanoclusters (Au / PtNCs) under different molar ratios of GSH to Au.

[0032] The results show that: when the molar ratio of GSH to Au is 10:1, that is, when the GSH in the reaction solution is 25 μmol. The fluorescence intensity of Au / PtNCs is the highest.

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Abstract

The invention discloses a gold / platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and an application of the fluorescence probe in aureomycin detection, and belongs to the technical field of fluorescence probes. A bimetal gold / platinum nanocluster has a great Stokes shift (about 150nm), can stably exist in a solution with extreme pH (potential of hydrogen) and high ion concentration, and releases stable fluorescence under long-time ultraviolet irradiation, so that a wide application of the nanocluster in the fields of medicines and biology is facilitated. In addition, the probe is used for detecting tetracycline antibiotics based on a mechanism of fluorescence quenching, and aureomycin can be distinguished from tetracycline by adding Al<3+> based on a mechanism of fluorescence enhancement, so that the purpose of specific aureomycin detection is achieved. The probe is used for detecting the aureomycin; a linear range is 0.5-30 micrometers; and a detection limit is 0.5 micrometers. In addition, real object detection is successfully performed on the aureomycin in milk by the fluorescence probe, and a good effect is obtained, so that the fluorescence probe can be used for detecting aureomycin residues in food.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a polyethylenimine-based gold / platinum bimetallic nano-cluster fluorescent probe and its application in detecting aureomycin. Background technique [0002] Because antibiotics have the ability to kill bacteria, they are widely used in the treatment of various diseases. However, the abuse or misuse of these antibiotics may lead to extremely serious side effects, such as allergic reactions, body resistance, etc., thereby reducing the effectiveness of treating infections. For example, tetracycline antibiotics (TCs) are an important class of broad-spectrum antibiotic drugs, including chlortetracycline (CTC), oxytetracycline (OTC), tetracycline (TC) and doxycycline (DC). A broad-spectrum bacteriostatic agent with a bactericidal effect at high concentrations. In addition to common Gram-positive bacteria, Gram-negative bacteria and anaerobic bacteria, most Ricke...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/87G01N21/64
CPCC09K11/025C09K11/87G01N21/643G01N2021/6432
Inventor 李洪伟吴玉清徐娜
Owner JILIN UNIV
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