Preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in blood system

A suspension cell and blood system technology, applied in the field of new materials, can solve the problems of difficult calibration and detection of suspension cells, and achieve the effects of easy operation, small size and simple preparation method

Active Publication Date: 2020-12-25
ZHONGYUAN ENGINEERING COLLEGE
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Problems solved by technology

[0004] The invention proposes a preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in the blood system, which solves the technical problems of difficult calibration and detection of suspended cells in the prior art. The invention discloses in detail a simple operation, Environmentally friendly, preparation method of platinum nanoclusters with excellent fluorescence properties, and expand the application range of fluorescent nanoplatinum clusters as markers in bioimaging, not only can calibrate adherent cells, but also can achieve optical imaging of suspended cells in the blood system, Realize early identification and diagnosis of blood system diseases

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  • Preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in blood system

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Embodiment 1

[0017]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The concentrations of and PEI were 0.1 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at 25 ℃ for 30 min, add 0.1 mmol L-ascorbic acid, heat the solution to 90 ℃, react for 2 h and then cool naturally to obtain a light yellow solution. After ultracentrifugation of the crude product and dialysis with a dialysis bag for 1 d, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted light blue fluorescence under ultraviolet light irradiation.

[0018]Fluorescence imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters finally obtained in the above step and add them to sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final fluorescent platinum nanocluster concentration is 0.001mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells ...

Embodiment 2

[0020]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing well at -4 ℃ for 24 h, add 0.1 mmolL-ascorbic acid, heat the solution to 90 ℃, react for 4 days and then cool naturally to obtain a yellow solution. After ultracentrifugation of the above crude product and dialysis with a dialysis bag for 2 days, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted yellow fluorescence under ultraviolet light irradiation.

[0021]Fluorescence imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters finally obtained in the above step and add them to sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final fluorescent platinum nanocluster concentration is 0.001mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells with 4% pa...

Embodiment 3

[0023]Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water2PtCl6And polyvinyl imine PEI, finally H2PtCl6The and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After fully mixing for 1.5 h at 60 ℃, add 0.1 mmol L-ascorbic acid, heat the solution to 90 ℃, react for 4 days and then cool naturally to obtain a yellow solution. After ultracentrifugation of the above crude product and dialysis with a dialysis bag for 2 days, the final fluorescent platinum nanoclusters were obtained, and the clusters emitted yellow fluorescence under ultraviolet light irradiation.

[0024]Fluorescence imaging of suspended cells in the blood system: Take the final fluorescent platinum nanocluster obtained in the above step and add it to sterile RPMI-1640 culture medium (containing 10% serum) containing BV173 cells. The final fluorescent platinum nanocluster concentration is 0.005 mmol / L. Pure air (5% CO2), after co-cultivation for 4 h, fix the suspended cells with 4% pa...

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Abstract

The invention belongs to the technical field of new materials, and especially relates to a preparation method and an application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells. The preparation method comprises the following steps: achieving cooperation of a platinum ion precursor aqueous solution and an amine polymer; performing full complexing by an amine group; adding a reducing agent at a high temperature to airtightly stir; preparing a crude product of the fluorescent platinum nanoclusters; and finally obtaining a water-soluble fluorescent platinum nanocluster after ultracentrifugation and dialysis A prepared fluorescent platinum nanocluster with an ultra-small size can be applied to the optical imaging of the blood system suspension cells. The invention provides the preparation method of the platinum nanoclusters which has the characteristics of simple operation, environmental protection, and excellent fluorescence performance. An adherent cell system can be calibrated; and the preparation method extends the platinum nanoclusters as fluorescent markers for the first time into the optical imaging of the blood system suspension cells, and is of great significance for early identification and diagnosis of blood system diseases.

Description

Technical field[0001]The invention belongs to the technical field of new materials, in particular to a preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in the blood system.Background technique[0002]In recent decades, the incidence of blood system diseases such as aplastic anemia and myeloproliferative diseases has been increasing year by year, and the harm caused by them has been increasing. For example, for leukemia, apart from bone marrow transplantation, no particularly effective treatment methods have emerged. Therefore, it is of great significance to study the early identification and diagnosis of hematopoietic tumor diseases. Noble metal nanoclusters, due to the scale close to the Fermi wavelength of electrons, the quantum confinement effect causes the original continuous electronic energy states to split into discrete electronic energy states, which are completely different from bulk materials and nanoparticles (gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/87C09K11/06B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00C09K11/06C09K11/87C09K2211/185G01N21/6486
Inventor 黄鑫罗毅岳献阳王少博于保康
Owner ZHONGYUAN ENGINEERING COLLEGE
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