94 results about "Post dialysis" patented technology

A method of performing regional citrate anticoagulant dialysis of a patient's blood includes flowing blood from and back to the patient through an extracorporeal circuit including a dialyzer having semi-permeable dialysis membranes and a dialysate chamber surrounding the membranes. The method further includes flowing a dialysate containing calcium and citrate through the dialysate chamber of the dialyzer and introducing citrate into the patient's blood upstream of the dialyzer, whereby the patient's blood is dialyzed. The method can further include predicting the concentration of systemic ionized calcium in the blood of the patient at any point in the dialysis treatment or post-dialysis, such as by a mathematical model. The method can further include statistically correcting the preliminary predicted post-dialysis concentration of systemic ionized calcium in the patient's blood to provide a final predicted post-dialysis systemic ionized calcium concentration. The method can further include statistically correcting the preliminary predicted systemic ionized calcium concentration for any time point during the dialysis treatment to provide a final predicted systemic ionized calcium concentration for that time point.

The invention discloses a fish skin collagen support loading epidermal growth factors and a preparation method thereof. The fish skin collagen support is composed of cross-linked fish skin collagen support bodies and nanometer particles of the load epidermal growth factors. The preparation method comprises the steps that after dewatering, degreasing, enzyme treatment and dialysis is conducted on fish skin, the cross-linked fish skin collagen support bodies are prepared, then the cross-linked fish skin collagen support bodies are soaked in a solution of the nanometer particles of the load epidermal growth factors, and the fish skin collagen support loading the epidermal growth factors is obtained after freezing and drying. The support has a slow releasing effect on the loaded factors, and prolongs the acting time of the epidermal growth factors at a trauma position. The fish skin collagen support obtained through the method has the advantages of being good in biocompatibility and biodegradability, free of immunogenicity and the like, is harmless to a wound surface, can effectively induce wound surface healing and is a good medical material.

The invention provides a single-excitation double-emission carbon-based biomass dot as well as a preparation method and application thereof. The preparation method comprise S1, a proper number of clean Chinese cabbage leaves are weighted, and are ground in a utensil; S2, after absolute ethyl alcohol and an acetone solution according to the volume ratio of 1:0.9 to 1:1.1 are added, and then the mixture is uniformly mixed for being soaked; S3, after being soaked for 28-32 minutes, filtering is conducted to obtain a chlorophyll extracting solution; S4, 25 mL of chlorophyll extracting solution proportional to 0.1g-0.11g of polyoxyethylene diamine are added in a polytetrafluoroethylene reaction kettle, and mixing and heating are conducted for 9-11h under the temperature controlled to be 195-205DEG C; S5, after cooling to the room temperature is conducted, suction filtration is conducted, and a filtrate is adjusted to that with a pH value roundly equal to 7; and S6, the filtrate is transferred into a dialysis bag for lucifugal dialysis for 11-13h to obtain the single-excitation double-emission carbon-based biomass dot. According to the dot and the preparation method and application thereof, the carbon-based biomass dot can be directly used as a probe for ratiometric fluorescence imaging of active substance coenzyme A in cells, the background fluorescence interference of biological tissue can be eliminated, and the accuracy and the sensitivity of the fluorescence imaging of the coenzyme A can be improved.

The invention discloses a preparation method of epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing. The method comprises the following steps: dissolving p-hydroxybenzene propanoic acid in a blended solvent of an organic solvent and deionized water, and stirring and uniformly mixing; adding 1-(3-dimethylamino propyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide, and performing activation in an ice-bath condition; adding epsilon-polylysine dissolved by deionized water into an activated system, so as to enable the epsilon-polylysine to react with the activated system at the room temperature; transferring an obtained system into a dialysis bag, so as to dialyze the system; freezing and drying dialyze purified solution, so as to obtain epsilon-polylysine-p-hydroxybenzene propanoic acid copolymer; respectively preparing a stock solution A and a stock solution B by taking PBS buffer solution as a solvent, respectively adding the stock solution A and the stock solution B into a tube A and a tube B of a double-end injector, and slowly pushing out the stock solution A and the stock solution B, so as to obtain the uniform and transparent epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing.

The invention relates to devices, systems, and methods for managing base levels, specifically bicarbonate, in an individual undergoing dialysis. The devices, systems, and methods can be compatible with a sorbent-based dialysis therapy, and open loop control of base levels in the dialysis, without sensors or feedback for controlling the introduction of bicarbonate into the dialysate. The bicarbonate levels can be controlled and personalized to a patient using certain predetermined patient parameters. Related systems, algorithms, and control systems are contemplated including the specific predetermined parameters predictive of post-dialysis bicarbonate levels.

The invention belongs to the field of biological medicine, relates to a specific high-affinity recombinant antibody marking fluorescent microsphere and a preparation method and application thereof and particularly relates to a plug-and-play fluorescence detection card for quick quantitative detection of KIM-1, NGAL, IGFBP7, TIMP-2, IL-18, PCT and cTnI in human urine or blood samples. The method includes: activating fluorescent microspheres; adding the activated fluorescent microspheres into a dialysis bag filled with phosphate buffer to obtain dialyzed fluorescent microsphere solution; adding amino-polyethylene glycol-carboxyl, further activating the fluorescent microsphere solution subjected to secondary dialysis, making with a recombinant antibody, centrifuging after reaction, washing, precipitating, resuspending and precipitating. The fluorescent microsphere has advantages that immunofluorescence reaction lower limit can be remarkably improved to enable sensitivity of a prepared test card to be improved by 10-100 times of that of test cards prepared according to a traditional method, and a test range is remarkably expanded.

The invention discloses an extraction and separation method of phyllanthus urinaria polyose PULP III, which comprises the following steps of removing dust by washing a whole phyllanthus urinaria plant with distilled water, drying, grinding, degreasing with petroleum ether, removing micromolecular sugar with ethanol, leaching medicine dregs with water, conducting centrifugal separation on leached liquid, concentrating filtrate, conducting ethanol precipitation with ethanol, conducting deproteinization, washing, purification and dialysis on an ethanol precipitate, drying, obtaining phyllanthus urinaria fine polyose, conducting deproteinization and dialysis, treating with an ion exchange column, eluting with appropriate NaCl gradient solutions in sequence, conducting track detection by a phenol-sulfuric acid method, obtaining an elution curve, collecting a third main peak component, concentrating, dialysing with redistilled water for 48h, freeze-drying, and obtaining a pure phyllanthus urinaria polyose PULP III component. According to the method, the pure and unique phyllanthus urinaria polyose component is obtained by separating and purifying crude phyllanthus urinaria polyose.

A hemodialysis machine comprising a fluid pathway for delivering a dialysate solution, wherein the fluid pathway comprises a pre-dialysis pathway and a post-dialysis pathway, a dialyser for dialysing patient's blood using the dialysate solution, the dialyser connected between the pre-dialysis pathway and the post-dialysis pathway, a first sensor system configured to sense a characteristic of the dialysate solution at a first location on the pre-dialysis pathway, a second sensor system configured to sense a characteristic of the dialysate solution at a second location on the post-dialysis pathway, and a control system configured to make a comparative analysis of the measurements taken by the first sensor and the second sensor for monitoring the composition of the dialysate solution.

The invention relates to the field of the preparation of monocrystalline silicon wafers, and discloses a preparation method of a chitosan-poly-2-acryloylamino-2-sodium methylpropyl sulfonate copolymerfor the surface texturing of monocrystalline silicon. The preparation method comprises the following steps of dissolving chitosan in deionized water, and under the protection of an inert gas, addingammonium ceric nitrate and 2-acryloylamino-2-sodium methylpropyl sulfonate in sequence; raising a temperature, reacting, then putting a reaction system into a dialysis bag, dialyzing in the deionizedwater, afterwards, freeze-drying, and collecting to obtain the copolymer. According to the preparation method, the chitosan-poly2-acryloylamino-2-sodium methylpropyl sulfonate copolymer prepared by the method is used for the surface texturing of the monocrystalline silicon; the preparation method is green and environment-friendly in material; further, the copolymer can be used for obviously improving the sedimentary ability, on the surface of a silicon wafer, of the copolymer on the premise that the deaeration effect of the copolymer is not influenced; an optimized textured-surface pyramid structure is maintained; tetrahedral square cones on a monocrystalline silicon wafer obtained after being subjected to the surface texturing are enabled to be uniform, and the reflectivity of the siliconwafer is low.

According to the hemostatic sponge cushion core and the preparation method thereof, a hemostatic sponge is smeared on a water absorption cushion in the foaming stage and does not disappear after being dried, and the hemostatic sponge cushion core can be further prepared into a functional dressing with an adhesive tape and is suitable for hemostasis and wound protection after human body surface dialysis and operation.

The invention provides a biomass carbon dot preparation method based on a sulfonic acid type eutectic solvent, which specifically comprises the following steps: respectively mixing chitin, bovine serum albumin and microcrystalline cellulose powder with choline chloride/p-toluene sulfonic acid, choline chloride/sulfamic acid, choline chloride/methane sulfonic acid, betaine/p-toluene sulfonic acid, betaine/sulfamic acid, choline chloride/methane sulfonic acid according to a mass ratio of 1: 10-1: 50, and reacting at 100-150 DEG C for 1-10 hours; after the reaction is finished, adding 50-100mL of water, performing standing for 12-24 hours, performing centrifugal separation to obtain supernate, transferring the supernate into a dialysis bag, putting the dialysis bag into an aqueous solution, and dialyzing for 24-48 hours; wherein after dialysis, the liquid obtained in the dialysis bag is the carbon dot aqueous solution.

The invention discloses new application of astragalus mongolicus lectin, which refers to application of the astragalus mongolicus lectin in inhibiting the growth of tumors, in particular in inhibitingthe proliferations of cervical cancer cells HeLa, leukemia cells K562 and osteosarcoma cells MG63. The astragalus mongolicus lectin is prepared by a method which comprises the following steps: 1) grinding astragalus mongolicus roots and then leaching the grinded astragalus mongolicus roots by the prior method, for instance, leaching the grinded astragalus mongolicus roots with a phosphate buffersolution, and then separating the supernatant to obtain an astragalus mongolicus crude extract; 2) salting the crude extract obtained in the step 1) out, for instance, performing sedimentation on thecrude extract with ammonium sulfate, dissolving sediments with a Tris-HCl buffer solution, performing dialysis on the dissolved solution, and centrifugating the solution obtained after the dialysis toobtain the supernatant; and 3) performing chromatography on the supernatant obtained in the step 2) by a ConA-Sepharose 4B affinity chromatographic column, performing dialysis on the solution obtained after the chromatography and condensing the solution obtained after the dialysis to obtain the astragalus mongolicus lectin.

The invention belongs to the technical field of new materials, and especially relates to a preparation method and an application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells. The preparation method comprises the following steps: achieving cooperation of a platinum ion precursor aqueous solution and an amine polymer; performing full complexing by an amine group; adding a reducing agent at a high temperature to airtightly stir; preparing a crude product of the fluorescent platinum nanoclusters; and finally obtaining a water-soluble fluorescent platinum nanocluster after ultracentrifugation and dialysis A prepared fluorescent platinum nanocluster with an ultra-small size can be applied to the optical imaging of the blood system suspension cells. The invention provides the preparation method of the platinum nanoclusters which has the characteristics of simple operation, environmental protection, and excellent fluorescence performance. An adherent cell system can be calibrated; and the preparation method extends the platinum nanoclusters as fluorescent markers for the first time into the optical imaging of the blood system suspension cells, and is of great significance for early identification and diagnosis of blood system diseases.

The invention discloses a preparation method of a silver doped carbon dot based NO release-type wound dressing, and a product and applications of the product. The preparation method includes as follows: placing a chlorhexidine acetate aqueous solution into a reactor, and performing reaction under high temperature; after the reactor is naturally cooled to room temperature, taking out the carbon dotsolution, dropwise adding the obtained solution into an AgNO3 solution, and performing reaction under the high temperature; obtaining a purplish red Ag/CDs composite solution after reaction, and adding L-arginine to stir after dialysis; and performing further dialysis, and performing freeze drying to obtain the silver doped carbon dot based NO release-type wound dressing. Compared with other wound dressings, the final dressing sample can react with H2O2 and functionally release NO; the wound dressing has multiple antibacterial mechanisms, so that antibacterial effects can be greatly enhanced;through bacteriostatic zone experiments, efficient antibacterial activity can be shown; and thus, the wound dressing can be widely applied to the disinfection and antibiosis of wounds, and also can reduce the risks of increased bacterial resistance.

The invention provides a dialysis method of a recombinant human tissue type plasminogen kinase derivative inclusion body enhancement solution, and application. The invention adopts ultrafilter dialysis, compared with dialysis bag dialysis, dialysis efficiency is obviously improved, dialysis time is shortened into 1/5 of original dialysis time, a use amount of dialysis external liquid is reduced to1/20 of an original use amount, the dialysed rPA solution is clear and transparent, and small molecule substances, including guanidine hydrochloride, reductant DTT and the like, can be more thoroughly removed; and after the ultrafilter dialysis, the obtained rPA solution is processed by subsequent steps, and the integral yield of produced rPA protein is improved by 20%. The invention provides a convenient and efficient method for renaturation purification of a large-scale industrial inclusion body, especially the inclusion body containing more disulfide bonds.

The invention discloses a formula and preparation method of a special dietary rice paste suitable for patients with nephropathy, and belongs to the technical field of food processing. The special dietary rice paste is composed of 45-50% of black rice powder, 11-14% of Chinese yam powder, 6-8% of lotus seed powder, 8-10% of poria cocos powder, 16-20% of milk powder, 8-10% of white granulated sugarand 1-2% of maltodextrin. The method comprises the following steps: crushing and sieving black rice, Chinese yams, lotus seeds and poria cocos, respectively adding 5% (g/mL) of maltodextrin, respectively preparing solutions by using purified water, performing fine grinding, and carrying out spray drying; and uniformly mixing the four dried powders with the milk powder, the white granulated sugar and the maltodextrin to obtain the special dietary rice paste. The special dietary rice paste is a purple gray powder, is in a uniform viscous paste state after being brewed, is smooth in mouth, and has rich fragrance of the black rice and faint scent of the lotus seeds. The preparation method of the product is simple. The rice paste is suitable for patients with nephropathy to eat after dialysis,provides energy, high-quality protein, micronutrients and the like required by the patients after dialysis, and can rapidly supplement nutrient substances lost in the bodies of the patients with nephropathy after dialysis.

The invention provides a preparation method and application of a non-metal water-soluble heavy metal adsorbent based on reversible phase transition. The preparation method of the adsorbent comprises the steps of (1) uniformly mixing melamine and an alkaline substance; (2) heating the mixture in the step (1) to the temperature of 250-600 DEG C, keeping the temperature for 1-5 hours, and cooling to room temperature; and (3) dissolving the solid obtained in the step (2) in ultrapure water, dialyzing in the ultrapure water through a dialysis bag, and drying the liquid in the dialysis bag to obtain the adsorbent. According to the technical scheme, raw materials are cheap and easy to obtain, the preparation process is simple, and secondary activation and other steps are not needed. The adsorbing material has a reversible phase inversion function which is not possessed by other adsorption materials. In addition, the mass transfer rate of an adsorption micro-interface is greatly improved, and the interface action between molecules and ions adsorbed in water is enhanced. No metal ion is needed in the preparation process of the adsorbing material, so that the adsorbing material is environment-friendly.

The invention discloses a preparation method of a long-acting and stable printing thickener, and belongs to the technical field of thickener preparation. Rosin reacts with tetrabutylammonium bromide and epoxy chloropropane to obtain viscous fluid, the viscous fluid is dissolved into an ethanol solution to be subjected to rotary evaporation treatment, a prepared dried product, a chitosan oligosaccharide solution and a sodium hydroxide solution are stirred for reacting, then, dialysis treatment is performed after citric acid is used for adjusting pH, then, the dialyzed reaction mixture and absolute ethyl alcohol are mixed and filtered through extraction, filter residues are frozen and dried to obtain the thickener, the thickener is stirred and mixed with methacrylic acid, maleic acid, ammonium persulphate and the like, ammonium persulphate is added for performing a heating reaction, and after cooling is performed, the long-acting and stable printing thickener can be obtained. The obtained thickener has the advantages that the consumption is small, the stability is good, the thickening effect is high, the electrolyte resistance is good, and bleeding is not likely to occur; after the thickener is adopted, no harmful effect can be generated on the hand feeling and color vividness of a fabric, and the preparation technology is simple and suitable for large-scale industrial production.

The invention discloses a method for efficiently preparing a lobaplatin anhydrous substance. The method comprises the following steps: by taking trans-1, 2-diaminomethyl-cyclobutane hydrochloride andpotassium chloroplatinite as raw materials and water as a solvent, firstly neutralizing the trans-1, 2-diaminomethyl-cyclobutane hydrochloride under an alkaline condition to dissociate trans-1, 2-diaminomethyl-cyclobutane, and then reacting with potassium chloroplatinite to generate dichloride; performing replacement reaction on dichloride and silver nitrate; filtering out generated silver chloride precipitates, reacting a filtrate with sodium lactate at a certain pH value, transferring a reaction liquid into a dialysis bag with the molecular weight cutoff of 200, dialyzing, concentrating anddrying to obtain the lobaplatin anhydrous substance. The method has the advantages of few operation steps, short synthesis time, simplicity in operation, high purity of the obtained lobaplatin anhydrous substance and high yield.

When a dialysate circuit 4 is cleaned after dialysis, both opening/closing valves V101 and V102 are closed after a dialysate in the dialysate circuit 4 is replaced with a cleaning liquid. The opening/closing valve V12 is opened, and the cleaning liquid is circulated by pumps P1 and P4 while being heated by a heater H1, to clean the inside of the dialysate circuit 4. A deaeration tank 31 is provided downstream of the pump P1. Air bubbles generated from the cleaning liquid serving as hot water are deaerated by the deaeration tank 31 and are then discharged into a gas storage tank 48 via a deaeration passage 32. The pumps P1 and P4 can be reliably prevented from being unable to operate with the air bubbles generated from the cleaning liquid.

The invention belongs to the field of biomass conversion, and discloses a catalyst for preparing 2-methyl furan through furfural gas phase hydrogenation, and a preparation method thereof. The catalyst is composed of a carrier SiO2 and an active component loaded on the carrier SiO2, and the loading amount of the active component is 10-40 wt%. And the active component is a metal simple substance Cu, Co or Ni. The preparation method comprises the following steps: mixing an active component precursor with water to prepare a precursor solution; then mixing the precursor solution and polyethylene glycol in proportion, and stirring for 10-60 min in an ice-water bath at 0-5 DEG C; maintaining the ice-water bath stirring condition, adding a NaBH4 solution, and continuously stirring for 2-4 h after adding is completed; carrying out dialysis treatment by adopting a dialysis bag; after dialysis, transferring the solution in the dialysis bag into a container, adjusting the pH value of the solution to 8-11 by adopting a NaOH solution, and then continuously stirring for 1-5 h; and adding a silicon source, stirring at 40-80 DEG C for 1-8 h, separating, washing, and drying to obtain the catalyst. The catalyst has high dispersity, high activity and high selectivity, the furfural conversion rate is 99%, and the 2-methyl furan selectivity is 90% or above.