44 results about "Post dialysis" patented technology

The invention relates to the technical field of biomedical materials, and discloses a soft tissue repairing and wrinkle filling material based on N-vinylpyrrolidone and polyethylene glycol diacrylate mixed polymer hydrogel and a preparation process of the soft tissue repairing and wrinkle filling material based on N-vinylpyrrolidone and polyethylene glycol diacrylate mixed polymer hydrogel. Polyethylene glycol diacrylate; an initiator; a solvent; the preparation process comprises the following steps: S1, mixing and dissolving all materials to form a pre-polymerization solution; s2, forming hydrogel through photopolymerization and thermal polymerization; s3, carrying out dialysis on the obtained hydrogel; and S4, performing freeze drying on the dialyzed hydrogel, and performing sterilization treatment. By adopting a light and heat dual initiation system, the purpose of realizing rapid polymerization under a mild condition is achieved. Compared with a single initiation system in the prior art, the technology solves the problems of slow polymerization rate and harsh reaction conditions, so that the polymerization process is more efficient and safer.

The invention relates to the technical field of dialysis process monitoring, in particular to a dialysis scheme self-adaptive interface system and method based on individual parameters of a patient, according to physiological state data and blood purification process data of the patient, the blood purification abnormal condition of the patient under the current dialysis scheme is analyzed; the acute complication risk of the patient under the current dialysis scheme is predicted; in combination with the physiological state data and the dialysis strategy data of the patient, analyzing the re-adaptation abnormal risk of purified blood and the body of the patient after dialysis under the current dialysis scheme; the assessment of the dialysis treatment safety risk of the patient under the current dialysis scheme is realized; therefore, self-adaptive adjustment and early warning can be carried out on the dialysis scheme of the patient. The reliability of dialysis treatment is improved, the treatment safety of a patient is guaranteed, and the occurrence risk of acute adverse events is reduced.

The application provides a blood viscosity detection method, device and equipment. The blood viscosity detection method comprises the following steps: inputting a replacement fluid to a vein pipeline through a hard pipeline at a first flow rate, and determining a first inlet and outlet pressure difference of the hard pipeline; determining the viscosity of the replacement fluid according to the pipeline attribute of the hard pipeline, the first flow rate and the first inlet and outlet pressure difference; if the difference between the viscosity of the replacement fluid and the viscosity of water is within a first preset range, then determining a viscosity coefficient according to the viscosity of water, the first flow rate and the first inlet and outlet pressure difference; performing dialysis on blood, and determining the second flow rate of the blood in the hard pipeline after dialysis and the second inlet and outlet pressure difference of the hard pipeline in real time; and determining the real-time viscosity of the blood during dialysis according to the viscosity coefficient, the second flow rate and the second inlet and outlet pressure difference. The application solves the problem that the blood viscosity cannot be detected during hemodialysis treatment in the prior art.

The application provides application of microgel particles taking cationic monomers as raw materials in preparation of blood crosslinking gel materials, and application of the microgel particles in preparation of interfacial stable hemostatic materials. The hemostatic gel prepared from the microgel particles has a sea-island belt structure based on blood cell bridging, and meanwhile, the strength and toughness of the material are improved. The gel can stably adhere to and support a wound, thereby achieving a dynamic hemostatic effect. The application fully utilizes the characteristics of large bleeding amount in severe bleeding, activates and anchors blood cells through an exogenous pathway based on electrostatic action. The application overcomes weakening effect of blood on a hemostatic material hemostatic interface, promotes formation of a blood enhanced phase, meets mechanical strength required for hemostasis at a severe bleeding site, and meets mechanical stability during patient transfer. The material used in the application has good biocompatibility after dialysis, has low toxicity and side effects on the human body, and provides great convenience for emergency hemostasis and the like.

The application provides a method and application for improving DNA yield in vitro amplification, and belongs to the technical field of biology. The application provides a method for improving DNA yield in vitro amplification, which comprises the following steps: (1) reacting a reaction system at 27-38 DEG C for 40-60 min; (2) adding the reaction liquid into a dialysis bag, adding dialysis liquid into an outer tube, and dialyzing at 30-38 DEG C; (3) supplementing tRNA into the reaction liquid for sealing after dialyzing for 1-3 h; the reaction system in step (1) comprises 5x synthesis enzyme mix, 5x synthesis reaction buffer, NTP, dNTP, a template and water; and the concentration of the dNTP is 0.1-0.5 mM. The method can significantly improve the DNA yield in vitro amplification, the concentration of the amplification product is increased by more than about 134 %, and the core problem of insufficient yield in the in vitro DNA amplification is effectively solved.

The application belongs to the technical field of biological medicine, and particularly relates to a cysteine-doxorubicin conjugate as well as a preparation method and application thereof. First, doxorubicin and 3-(2-pyridyl dithio) propionic acid are dissolved in dichloromethane, then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride is added, and after stirring and dissolving at room temperature, heating and stirring reaction is performed. After the reaction is completed, the solvent is spun dry, and the 3-(2-pyridyl dithio) propionic acid doxorubicin ester is obtained through column chromatography separation and purification. Then, the 3-(2-pyridyl dithio) propionic acid doxorubicin ester is dissolved in N,N-dimethylformamide, cysteine is added, and after stirring and dissolving at room temperature, heating and stirring reaction is performed. After the reaction liquid is cooled to room temperature, it is added to a dialysis bag and dialyzed in ultrapure water. After freeze-drying, the cysteine-doxorubicin conjugate is obtained.

The present application relates to the field of synthetic biology, and specifically discloses a preparation method for improving the biological activity of lentinan and application thereof. The method adopts white shiitake mushroom to perform solid-state fermentation on lentinan, and comprises the following steps: S1, grinding the lentinan into powder, then adding appropriate amount of water to stir uniformly, high-pressure sterilization, and cooling to obtain lentinan culture medium; S2, activating white shiitake mushroom strains and preparing white shiitake mushroom seed liquid, adding the white shiitake mushroom seed liquid into the lentinan culture medium according to a certain inoculation amount to perform solid-state fermentation; S3, taking the fermented lentinan culture medium, performing protease enzymolysis first, then adopting ethanol precipitation, centrifuging to take the precipitate, resolubilizing the precipitate, performing dialysis treatment through a dialysis bag, and freeze-drying the dialyzed solution to obtain lentinan. The preparation method significantly improves the yield and purity of lentinan, and at the same time, the antioxidant, anti-inflammatory and pancreatic lipase clearance abilities are significantly improved, and the method has the effect of lubricating intestines.

The invention discloses a preparation method of chitosan surface modified extracellular vesicles, which specifically comprises the following steps: S1, dissolving chitosan in water, adjusting the pH value to be acidic, and completely dissolving the chitosan to obtain a chitosan solution with the concentration of 10mg / mL; s2, adding N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride into the solution obtained in the step S1, and uniformly stirring and fully mixing the N-hydroxysuccinimide and the 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride; s3, the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 is dissolved in formamide, and a distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 solution with the concentration being 1 mg / mL is prepared; s4, uniformly mixing the solution obtained in the step S2 and the solution obtained in the step S3, and stirring at room temperature for 12 hours; s5, transferring the mixed solution obtained in the step S4 into a dialysis bag with the specification of 3.5 KDa, and dialyzing in pure water for 24 hours; and S6, co-incubating the dialyzed solution obtained in the step S5 with an extracellular vesicle solution, and self-assembling to form the extracellular vesicle with the chitosan surface modified. The chitosan surface modified extracellular vesicles prepared by the method disclosed by the invention have relatively high encapsulation efficiency on hydrophobic drugs such as fucoxanthin and the like and relatively high stability in various environments. The method is suitable for embedding and delivering various bioactive substances, and has an important application prospect in the aspect of active substance delivery.

The invention belongs to the field of biological medicine, and particularly relates to preparation and application of dextran for relieving ulcerative colitis. The dextran GS128 is formed by directly catalyzing and synthesizing dextransucrase Gtf128 generated by heterologous expression in vitro by taking sucrose as a substrate, and the product is obtained by ethanol precipitation, centrifugation, dissolution and dialysis. The compound shows high anti-digestion performance in the process of simulating gastrointestinal tract digestion, and can relieve the symptoms of ulcerative colitis by inhibiting oxidative stress, adjusting the balance of proinflammatory / anti-inflammatory factors and improving the colon barrier.

The invention discloses a novel application with a cold compress hemostasis function for hemodialysis, and relates to the technical field of medical supplies. The plaster comprises a plaster body, a first opening is formed in the plaster body, internal threads are formed in the upper side of the plaster body and located in the first opening and the inner wall of a mounting head, and bandages used for binding are arranged on the two sides of the plaster body; the hydrocolloid dressing layer is attached to the lower side of the application body. The hydrocolloid dressing layer can be directly and firmly adhered to the skin at the edge of a wound and can absorb seepage, the pressing cylinder is arranged and moves downwards by rotating the pressing cylinder so as to slowly press and stop bleeding of the wound, and the cold insulation ring is arranged so that local blood vessels around the wound can be conveniently constricted. And the traditional Chinese medicine preparation can be immediately used after dialysis, so that the wound can be scientifically nursed, and the pain of a patient can be relieved.

The invention discloses an intelligent dialysis service method and system, and provides an intelligent dialysis service system capable of monitoring physiological information of a patient in real time, judging the dialysis effect, establishing a dialysis model, performing risk assessment and optimizing a treatment scheme. Firstly, physiological information of a patient is monitored in real time through a physiological parameter monitoring module, and data is fed back to a server. The dialysis scheme generation module is used for judging the dialysis effect according to the physiological information after dialysis, generating corresponding dialysis effect description data, establishing a dialysis model based on the generated data, analyzing the physiological information before and after dialysis, obtaining dialysis condition data, generating warning information through the dialysis condition analysis module, and assisting a doctor in judging the state of a patient. According to patient monitoring data analysis and dialysis effect description, abnormal conditions are found in time through an abnormal monitoring and early warning module, risk assessment is carried out, and early warning standards of different levels are set, so that medical staff can carry out corresponding processing.

The present application relates to hemodialysis prescription optimization technical field, especially in a kind of method and device for determining safe and effective prescription interval of hemodialysis.The method is in two-dimensional parameter space with ultrafiltration volume and dialysate sodium concentration as coordinate axis, using double-chamber sodium dynamics model (diffusion equilibrium concentration C_eq=[Na]d / σ (σ=0.97)) to calculate the sodium removal of each parameter combination and serum sodium after dialysis, superimpose sodium removal target line, ultrafiltration rate safety line and serum sodium safety area three constraint boundaries, the area that meets all constraints simultaneously is determined as safe and effective prescription interval.The safe and effective prescription interval is obtained by mathematical model calculation, not determined directly by preset empirical threshold.The present application first proposes the concept of safe and effective prescription interval, upgrades dialysis prescription from single-point empirical selection to interval optimization selection, and the constraint boundary can be dynamically adjusted according to individualized parameters of patients, to provide intuitive multi-constraint prescription decision support tool for clinicians.

The invention discloses a preparation method of a surface modified chitin whisker, which comprises the following steps: taking a proper amount of chitin to carry out acidolysis reaction, removing supernate through a centrifugation method after the reaction is finished, cleaning, then adding the acidolysis chitin into purified water, then adding sodium hydroxide or potassium hydroxide and chloroacetic acid according to a ratio, setting reaction conditions, and carrying out solid-liquid separation to obtain the surface modified chitin whisker. After the reaction is finished, diluting and standing, washing by adopting a centrifugal method to obtain a residual solid, adding water, dialyzing, carrying out ultrasonic treatment, freeze-drying and the like. On the basis of retaining the key structure of the chitin whisker, the surface-modified chitin whisker which is more excellent in dispersity and compatibility than the chitin whisker is simply and efficiently prepared, and meanwhile, a centrifugal-dialysis combined purification technology is matched, so that the reaction period is greatly shortened, the production cost is greatly reduced, and the method is suitable for industrial production. The application prospects of the chitin whiskers in the aspects of beauty makeup, daily chemicals and medicines are greatly improved.

The application belongs to the field of nanomedicine, and discloses a dual-target ROS-responsive nanomedicine for treating MASH and a preparation method thereof, which comprises the following steps: step 1, organic phase preparation, MCC950, Belzutifan and PEG-b-PPS are added into an organic solvent, and ultrasonic treatment is performed until complete dissolution to form an organic phase, the average molecular weight of the PEG block in PEG-b-PPS is 1000-5000, and the polymerization degree of the PPS block is 10-100; step 2, self-assembly wrapping, the organic phase is slowly added into an aqueous phase under constant magnetic stirring, and a primary micelle emulsion is obtained after self-assembly; step 3, dialysis purification and drying, the primary micelle emulsion is transferred into a dialysis bag for dialysis, and then freeze-drying is performed to obtain a dual-target nanomicelle powder. The dual-target ROS-responsive nanomedicine at least comprises the dual-target nanomicelle powder, the medicine breaks through the bottleneck of dissolution and delivery of extremely hydrophobic drugs, has excellent intravenous drug adaptability and stability, can realize long-acting and precise liver targeting enrichment, and greatly reduces the risk of systemic off-target toxicity.

The invention relates to the technical field of medicines, in particular to a nitric oxide-driven nano motor composition as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving cyclodextrin and Boc protected arginine in DMF, and adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing, freeze-drying and separating to obtain a white product CD-Arg; the preparation method comprises the following steps: dissolving rapamycin and thioketal in anhydrous DMF, and adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing, freeze-drying and separating to obtain a yellow product RAPA-TK; the preparation method comprises the following steps: dissolving CD-Arg and RAPA-TK in DMF, and then adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing the product, and freeze-drying and separating to obtain the nano-motor composition. The nano motor provided by the invention has special chemical bonds sensitive and responsive to an inflammatory microenvironment, and is easy to break in the inflammatory environment, so that targeted drug release can be realized.

The invention is applicable to the technical field of biology, and provides a pretreatment method of a protein sample and a peptide diagram analysis method, which comprises the following steps: (a) carrying out a heating denaturation reduction reaction on the protein sample in the presence of a denaturing agent and a reducing agent, and then adding an alkylation reagent to carry out a dark alkylation reaction; (b) filling the reaction liquid into a dialysis bag, and carrying out multiple times of liquid change dialysis; (c) concentrating the dialyzed sample solution, and adding a protease solution to carry out enzymolysis reaction; (d) adding an acid solution into the solution after enzymolysis to terminate the reaction, centrifuging and taking supernate, the method overcomes the problem of loss of a membrane adsorption sample caused by a traditional ultrafiltration method, is particularly suitable for pretreatment of a trace protein sample, reduces the use amount of the sample and the experiment cost, can be compatible with synchronous digestion reaction of multiple enzymes, and has a wide application prospect. The pretreated sample is analyzed by LC-MS / MS, a chromatogram with good peak shape and good separation degree can be obtained, and reliable data is provided for quality control and structural identification of protein drugs.

The present invention discloses a preparation method and application of artificial peroxisomes LOX-Ce6-Mn; belonging to the field of nanomaterials and tumor treatment technology, the preparation steps are: firstly uniformly mixing prepared DCC, NHS, Ce6, and LOX in proportion, dialyzing with a dialysis bag after the reaction, adding an aqueous solution of MnCl2, adjusting the pH value, dialyzing, and drying to obtain LCM nanoparticles. In the present invention, the lactate oxidase activity, oxidase activity (OXD), peroxidase activity (POD), and catalase activity (CAT) of the LCM nanoparticles can constitute a cascade reaction, causing tumor cells to produce efficient reactive oxygen and consume intratumoral lactate and glutathione, causing tumor cells to undergo autophagy and apoptosis; in addition, the H2O2 catalyzed by LOX can be transported by Mn 2+ The mediated Fenton-like reaction converts hydroxyl radicals into highly toxic free radicals, further amplifying the oxidative damage of cancer cells. LCM nanoparticles are tumor-specific in killing tumor cells, effectively accumulating in tumor sites, with little killing effect on normal sites, and have good biocompatibility.

The invention discloses a nano material for inhibiting aseptic inflammation as well as a preparation method and application of the nano material. The nano material is hybrid liposome nano vesicles modified by a platelet membrane, and the mass ratio of protein to lipid is 1: 2. The nano vesicles are loaded with sylvite compounds, can target in-vivo tissue injury parts, release potassium ions under the action of ultrasound, realize intracellular pH regulation and control through hydrogen-potassium exchange, and inhibit aseptic inflammation. The hybrid liposome is formed by mixing phospholipid and cholesterol, and the potassium salt compound is potassium carbonate or potassium bicarbonate. The preparation method comprises the following steps: preparing a hybrid liposome membrane and a platelet membrane, respectively adding a sylvite compound solution to carry out ultrasonic hydration and resuspension washing, preparing hybrid liposome vesicles and platelet membrane vesicles entrapped with sylvite compounds, and carrying out ultrasonic treatment, size stabilization and dialysis to obtain the platelet membrane modified hybrid liposome nano-vesicles. Inflammation can be inhibited in the early stage of injury, and side effects of traditional drugs are avoided.

The invention relates to the technical field of medical instruments, and provides a dialysis urea clearance rate online monitoring method, a computer system and a monitoring system.The monitoring method comprises the steps that S1, a detection light source is adopted to irradiate dialysis waste liquid, and the incident light intensity I0 is recorded; s2, collecting the initial transmission light intensity I1 penetrating through the dialysis waste liquid and the transmission light intensity It at the current t moment, and calculating the ratio Rt of the urea nitrogen concentration after dialysis to the urea nitrogen concentration before dialysis on the basis of I1 and It; and S3, calculating a urea clearance index Kt / Vt value at the current t moment based on the Rt. The method has the advantages that the final urea clearance index Kt / Vt value is obtained according to the data of the illumination intensity of the dialysis waste liquid, real-time monitoring of urea data is achieved, the accuracy of the final urea clearance rate calculation result is improved, medical staff can conveniently and timely obtain the urea clearance condition of a dialyzer on blood, and the medical staff can conveniently and rapidly know the urea clearance condition of the dialyzer on the blood. In addition, invasive operation in a traditional blood sampling measurement mode is avoided, discomfort brought to a patient is avoided, and the infection risk is reduced.

The invention relates to a sericin-akermanite composite nerve conduit. A preparation method of the sericin-akermanite composite nerve conduit comprises the following steps: dissolving silkworm cocoons with a lithium bromide solution to obtain a sericin solution; the sericin solution and the Tris-Hcl aqueous solution are mixed and added into a dialysis bag; adding the dialysis bag into the dialysate for dialysis; centrifuging, filtering and collecting the dialyzed sericin solution; adding the sericin solution into a polyethylene glycol supersaturated solution, and concentrating; adding akermanite into the genipin solution to prepare a cross-linking solution; mixing the sericin solution and the cross-linking solution, upside down and uniformly mixing, and injecting into a mold; and incubating to obtain the sericin hydrogel. And freezing the sericin hydrogel, demolding and freeze-drying to obtain the sericin-akermanite composite nerve conduit. According to the present invention, the cell compatibility, the degradability and the immunogenicity are good; proliferation and migration of nerve regeneration key cells-Schwann cells and secretion of various nutritional factors can be promoted; and the structural regeneration and functional recovery of long-distance peripheral nerve dissection are promoted.

The invention provides ruthenium-curcumin-based nano-enzyme, a preparation method thereof and application of the ruthenium-curcumin-based nano-enzyme in radiation-induced oral mucositis, and belongs to the technical field of biological medicine. The method comprises the following steps: mixing a ruthenium source methanol solution and a dispersant methanol solution to obtain a ruthenium source dispersion liquid; and mixing the ruthenium source dispersion liquid, curcumin and o-phenylenediamine, carrying out a self-assembly reaction under a stirring condition, and carrying out solid-liquid separation and dialysis to obtain the ruthenium-curcumin-based nano-enzyme. Experiments prove that the ruthenium-curcumin-based nano-enzyme has SOD, CAT, OH and O2 <-> removal performance, inhibits endoplasmic reticulum stress and reduces mitochondrial calcium overload, so that oxidation circulation from endoplasmic reticulum to mitochondria is blocked, and radiation-induced oral mucositis is treated.

The application belongs to the technical field of medical polymer hemostatic materials, and particularly relates to a gelatin / polyacrylic acid / NHS-AAc composite in-situ hemostatic hydrogel patch and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a gelatin solution; (2) adding linear polyacrylic acid and NHS-AAc into the gelatin solution and stirring to obtain a precursor solution; (3) adding ammonium persulfate into the precursor solution and stirring to obtain a mixed solution; (4) transferring the mixed solution into a mold, standing and polymerizing to obtain a molded hydrogel; (5) taking the molded hydrogel out of the mold, placing the molded hydrogel in a dialysis bag and dialyzing; and (6) drying the dialyzed hydrogel and packaging. The hydrogel patch of the application can maintain excellent water absorption and hemostatic efficiency, significantly enhance the structural stability in a high-salt environment, and has good biological safety.

The utility model discloses an internal arteriovenous fistula nursing device, which relates to the technical field of nursing and comprises a sleeve, a fixing component is arranged on the surface of the sleeve, and a binding component is arranged on the surface of the sleeve. The oversleeves are arranged on the arms of a patient in a sleeving mode, the two ends of each oversleeve are not arranged in an elastic mode, the patient can wear the oversleeves more easily, the skin of the patient can be isolated from clothes through the oversleeves, and the clothes are prevented from rubbing the patient; the medical ice bag can be inserted between the first elastic band and the second elastic band to be fixed, so that the acupuncture position of a patient is subjected to cold compress, the sleevelet can block the medical ice bag, discomfort caused by long-time contact with the medical ice bag is prevented, and when the patient needs to wear the medical ice bag for a long time, the medical ice bag is fixed. The bandage connected with the connecting belt is wound on the surface of the sleeve and covers the medical ice bag, and the hook-and-loop fastener is in contact with the hook-and-loop fastener, so that the sleeve is fixed by the bandage, the medical ice bag is prevented from falling off, and the adaptability of the device is improved.

The application discloses application of black corn polysaccharide in preparation of a medicine for preventing or treating senile osteoporosis. The evaluation of the prevention or treatment effect of the black corn polysaccharide on senile osteoporosis includes improvement effects on serum bone metabolism markers, bone microstructure and bone biomechanics. The black corn polysaccharide of the application refers to a polysaccharide powder after water extraction, alcohol precipitation, Sevag method deproteinization, H2O2 method depigmentation and dialysis bag dialysis from dried black corn kernels. The black corn polysaccharide has improvement effects on imbalance of serum bone metabolism markers of senile osteoporosis, deterioration of bone microstructure and decrease of bone strength. The results show that the black corn polysaccharide has great improvement effects on bone loss in preventing senile osteoporosis. Therefore, the black corn polysaccharide has important significance in developing senile osteoporosis in clinic.

The application discloses a preparation method and application of a nano preparation targeting a sepsis microenvironment and belongs to the technical field of pharmaceutical preparations. The specific method is as follows: (1) PLGA-COOH is mixed with EDC and NHS and reacted under certain conditions for a period of time to obtain a mixed solution A, then the mixed solution A is mixed with PSA and reacted, dialysis is carried out by using a dialysis bag, and then freeze-drying, re-dissolving, centrifugation and repetition of three times are carried out to obtain a conjugate PSA-COO-PLGA; (2) a scophularia derivative is mixed with the conjugate PSA-COO-PLGA according to a certain proportion and stirred, then ultrasonic treatment is carried out under ice bath conditions, filtration and freeze-drying are carried out to obtain the nano preparation PSA-COO-PLGA / drug NPs. The functional nano carrier prepared in the application can load scophularia alkaloid drugs, increase the stability of the drugs, prolong the blood circulation time of the drugs, increase the water solubility of the scophularia alkaloid compounds and enhance the safety of the drugs.

The invention relates to the field of medical beauty materials, in particular to injectable cross-linked recombinant collagen gel for medical beauty and a preparation method of the injectable cross-linked recombinant collagen gel. Mixing the recombinant collagen with glutamine transaminase, and carrying out cross-linking reaction to form gel; carrying out dialysis on gel formed by crosslinking in normal saline at the temperature of 2-8 DEG C for 12-24 hours so as to remove free glutamine transaminase; diluting the dialyzed gel to a target concentration; and filling the diluted gel, and carrying out terminal moist heat sterilization under the conditions that the temperature is 121 DEG C and the standard sterilization time is greater than or equal to 12 minutes, so that the gel is homogenized. According to the technical scheme, through specific crosslinking and subsequent process treatment, the obtained gel can tolerate terminal moist heat sterilization conditions that the temperature is 121 DEG C and the F0 is greater than or equal to 12 minutes. Therefore, an expensive and high-risk sterile filling process can be abandoned in production, but a terminal sterilization process with more consistent batch sterility guarantee level is adopted, so that the production cost and the quality risk are remarkably reduced.

The invention relates to the technical field of medicine, and discloses hyaluronic acid-silk fibroin hydrogel injected into a bone joint cavity and a preparation method of the hyaluronic acid-silk fibroin hydrogel. The gel particles contain hyaluronic acid or salt thereof, silk fibroin and a cross-linking agent; the cross-linking agent is BDDE; the molecular weight of the silk fibroin is 2.5-100 thousand Daltons; the preparation method comprises the following steps: a, dissolving a certain amount of hyaluronic acid or salt thereof and silk fibroin powder in an alkaline solution, uniformly stirring, and carrying out defoaming treatment; b, adding a proper amount of cross-linking agent BDDE into the solution defoamed in the step a to form a mixed solution, carrying out cross-linking, and then dialyzing; c, the gel obtained after dialysis in the step b is sieved through a 40-100-mesh sieve to be granulated, and gel particles are obtained; and d, adding a proper amount of aqueous medium into the gel particles in the step c for resuspension to obtain the hyaluronic acid-silk fibroin hydrogel. The hydrogel disclosed by the invention has excellent mechanical properties, viscoelasticity and abundant porous structures, is remarkable in anti-inflammatory effect, can promote the growth of cartilage cells, is used for intraarticular injection and treatment of osteoarticular related diseases, and has a better industrial production prospect.

The utility model relates to the technical field of hemodialysis instruments, in particular to an integrated forward pre-flushing hemodialysis pipeline which comprises a three-way connector, and three connectors of the three-way connector are a hemodialysis machine connector directly connected with a hemodialysis machine, a hemodialysis pipe connector connected with an artery end hemodialysis pipeline and a pre-flushing end connector communicated with a pre-flushing pipeline. The pre-flushing pipeline and the artery pre-flushing pipeline are directly connected to the blood inlet end of the dialysis machine through the three-way connector, and the problems that during pre-flushing before dialysis, the number of operation steps is increased, and the risk that a person pollutes a link opening is caused are solved; and when blood is returned after dialysis, the problems that blood is returned at the front end of the artery under natural pressure during blood return, the blood can exceed the access end of the pre-flushing pipeline, the blood cannot enter when the internal fistula pressure is high, and blood coagulation possibly occurs when the pump is stopped to return the arterial blood when no anticoagulant or less anticoagulant exists are directly avoided.

The invention provides an automatic grading system and method for the blood coagulation degree after hemodialysis, and the system comprises a multispectral imaging module which is used for collecting multispectral image data of a blood channel assembly according to a time sequence; a multi-modal feature fusion module connected with the multi-spectral imaging module and configured to receive the multi-spectral image data and perform preprocessing and multi-modal feature fusion; the AI blood coagulation analysis module is connected with the multi-modal feature fusion module, and is configured to receive the fused features, perform space and time sequence modeling by using a convolutional neural network and a long-short-term memory network, and output blood coagulation grade and trend early warning based on a modeling result; the display control module is used for displaying the blood coagulation level and trend early warning information; according to the system and the method, full-automatic and high-precision quantitative grading and trend early warning of the blood coagulation degree after hemodialysis can be realized.

The method comprises the following steps: (1) synthesizing a HA-AEMA sample: dissolving sodium hyaluronate in a PBS buffer solution, adjusting the pH value to 7.0, adding activators NHS and EDC to activate carboxylic acid groups of sodium hyaluronate, then adding 2-aminoethyl methacrylate hydrochloride, dissolving in PBS, adding into a reaction system, reacting at the temperature of 60-80 DEG C for 1-2 hours, and filtering to obtain the HA-AEMA sample; after reacting for 24 hours, dialyzing and freeze-drying to obtain a photo-crosslinkable HA-AEMA sample; (2) preparation of a drug loading solution: dissolving 5% of the HA-AEMA sample and puerarin in a methanol-water mixed solvent, adding 0.01% of a photoinitiator LAP, and uniformly mixing to obtain the drug loading solution; and (3) photo-crosslinking molding: injecting the drug-loaded solution into a mold, and irradiating with blue light for 10-20 minutes to form the puerarin hyaluronic acid hydrogel. The method has the advantages of few preparation steps, lower experimental cost, convenience in operation and stable preparation process.