62 results about "Post dialysis" patented technology

The invention relates to the technical field of medical instruments, in particular to an internal fistula blood vessel detection system for hemodialysis, which comprises a data acquisition module for acquiring ultrasonic data, an impedance value, tremor intensity and a pressure spectrum; the mode determining module is used for determining a detection mode of the internal fistula blood vessel based on the tortuosity index and determining massage frequency and air bag pressure according to the blood vessel compliance index; the stenosis analysis module is used for determining whether the internal fistula blood vessel is stenosis or not based on the stenosis risk characterization parameter and determining whether the stenosis degree of the internal fistula blood vessel is in a reasonable range or not according to the blood flow velocity characterization value; the frequency adjusting module determines and adjusts the massage frequency based on the ratio; the injury analysis module is used for determining whether the internal fistula blood vessel is injured or not in the dialysis process on the basis of the bioelectrical impedance change rate and determining whether the internal fistula blood vessel injury is in a reasonable range or not after dialysis according to the tremor intensity change value; the pressure adjusting module determines and adjusts the pressure of the air bag based on the difference value. The accuracy of internal fistula blood vessel detection is improved.

The invention relates to the technical field of biomedical materials, and discloses a soft tissue repairing and wrinkle filling material based on N-vinylpyrrolidone and polyethylene glycol diacrylate mixed polymer hydrogel and a preparation process of the soft tissue repairing and wrinkle filling material based on N-vinylpyrrolidone and polyethylene glycol diacrylate mixed polymer hydrogel. Polyethylene glycol diacrylate; an initiator; a solvent; the preparation process comprises the following steps: S1, mixing and dissolving all materials to form a pre-polymerization solution; s2, forming hydrogel through photopolymerization and thermal polymerization; s3, carrying out dialysis on the obtained hydrogel; and S4, performing freeze drying on the dialyzed hydrogel, and performing sterilization treatment. By adopting a light and heat dual initiation system, the purpose of realizing rapid polymerization under a mild condition is achieved. Compared with a single initiation system in the prior art, the technology solves the problems of slow polymerization rate and harsh reaction conditions, so that the polymerization process is more efficient and safer.

The invention relates to the technical field of dialysis process monitoring, in particular to a dialysis scheme self-adaptive interface system and method based on individual parameters of a patient, according to physiological state data and blood purification process data of the patient, the blood purification abnormal condition of the patient under the current dialysis scheme is analyzed; the acute complication risk of the patient under the current dialysis scheme is predicted; in combination with the physiological state data and the dialysis strategy data of the patient, analyzing the re-adaptation abnormal risk of purified blood and the body of the patient after dialysis under the current dialysis scheme; the assessment of the dialysis treatment safety risk of the patient under the current dialysis scheme is realized; therefore, self-adaptive adjustment and early warning can be carried out on the dialysis scheme of the patient. The reliability of dialysis treatment is improved, the treatment safety of a patient is guaranteed, and the occurrence risk of acute adverse events is reduced.

The invention discloses a preparation method and application of Cu / N-CDs, and relates to the technical field of Cu / N-CDs preparation. The preparation method comprises the following steps: adding copper citrate, ethidene diamine and ultrapure water together, and ultrasonically stirring uniformly; transferring the obtained solution into a polytetrafluoroethylene reaction kettle, then placing the polytetrafluoroethylene reaction kettle in an electrothermal forced air drying oven, and carrying out hydrothermal reaction at 180 DEG C; naturally cooling, controlling the reaction liquid to be centrifuged, and filtering by adopting a microfiltration membrane; dialyzing the filtrate in ultrapure water for 24 hours by using a dialysis bag, and replacing new ultrapure water every 6 hours; and freeze-drying the dialyzed filtrate to obtain light brown solid powder, and marking the light brown solid powder as Cu / N-CDs. According to the preparation method, a one-step hydrothermal method is adopted, copper citrate and ethylenediamine serve as precursors, the prepared copper-nitrogen co-doped carbon quantum dots show excellent peroxidase-like catalytic activity through the synergistic effect of Cu < + > / Cu < 2 + > redox pairs, and the catalytic efficiency is remarkably improved compared with that of traditional nano-enzymes.

The invention is suitable for the technical field of sonodynamic antibacterial materials, and provides a preparation method of a polyphenol nanodot sonosensitizer and application of the polyphenol nanodot sonosensitizer in sonodynamic antibiosis. The method comprises the following steps: dissolving tannic acid (TA) in deionized water, stirring at room temperature until the tannic acid is completely dissolved, carrying out oxidative polymerization reaction on the dissolved solution, assembling the tannic acid through oxidative polymerization to obtain the polyphenol nanodot sound-sensitive agent (TANDs), cooling after the reaction is finished, collecting the product, filtering, transferring the filtrate into a dialysis bag for dialysis, changing water once every 6 hours, and drying to obtain the polyphenol nanodot sound-sensitive agent (TANDs). Freeze-drying the dialyzed solution into solid powder, and dispersing the freeze-dried solid powder into deionized water for later use; tA is used as an element, the TANDs with the sound-sensitive property are prepared through simple oxidative polymerization reaction self-assembly, the TANDs show excellent singlet oxygen (1O2) generation capacity under the ultrasonic (US) condition, and the TANDs also show excellent antibacterial performance in the actual antibacterial application process.

The application provides a blood viscosity detection method, device and equipment. The blood viscosity detection method comprises the following steps: inputting a replacement fluid to a vein pipeline through a hard pipeline at a first flow rate, and determining a first inlet and outlet pressure difference of the hard pipeline; determining the viscosity of the replacement fluid according to the pipeline attribute of the hard pipeline, the first flow rate and the first inlet and outlet pressure difference; if the difference between the viscosity of the replacement fluid and the viscosity of water is within a first preset range, then determining a viscosity coefficient according to the viscosity of water, the first flow rate and the first inlet and outlet pressure difference; performing dialysis on blood, and determining the second flow rate of the blood in the hard pipeline after dialysis and the second inlet and outlet pressure difference of the hard pipeline in real time; and determining the real-time viscosity of the blood during dialysis according to the viscosity coefficient, the second flow rate and the second inlet and outlet pressure difference. The application solves the problem that the blood viscosity cannot be detected during hemodialysis treatment in the prior art.

The application provides application of microgel particles taking cationic monomers as raw materials in preparation of blood crosslinking gel materials, and application of the microgel particles in preparation of interfacial stable hemostatic materials. The hemostatic gel prepared from the microgel particles has a sea-island belt structure based on blood cell bridging, and meanwhile, the strength and toughness of the material are improved. The gel can stably adhere to and support a wound, thereby achieving a dynamic hemostatic effect. The application fully utilizes the characteristics of large bleeding amount in severe bleeding, activates and anchors blood cells through an exogenous pathway based on electrostatic action. The application overcomes weakening effect of blood on a hemostatic material hemostatic interface, promotes formation of a blood enhanced phase, meets mechanical strength required for hemostasis at a severe bleeding site, and meets mechanical stability during patient transfer. The material used in the application has good biocompatibility after dialysis, has low toxicity and side effects on the human body, and provides great convenience for emergency hemostasis and the like.

The invention provides peritoneal dialysis positioning equipment for the nephrology department, and belongs to the technical field of medical instruments. Comprising a dialysis tube fixing module, a left side foundation plate, a right side foundation plate and a small ultrasonic detection module, the left side foundation plate is arranged on the left side of the dialysis tube fixing module, the right side foundation plate is arranged on the right side of the dialysis tube fixing module, and vertical sliding grooves are formed in the front faces of the left side foundation plate and the right side foundation plate; the inner wall of the vertical sliding groove is in sliding connection with a small ultrasonic detection module. By arranging the small ultrasonic detection module, the device can directly perform ultrasonic detection on the position of a dialysis tube in the body at home after or before dialysis of a patient, so that the patient does not need to go to a hospital for multiple times to perform ultrasonic detection; in this way, the time consumed when the patient goes back and forth to the hospital and waits in the hospital is shortened to a certain extent, and meanwhile energy consumption caused by queuing registration of the patient can be reduced.

The invention relates to synthesis of a human homocysteine hapten and a preparation method and application of an antibody of the human homocysteine hapten. The synthesis of the human homocysteine hapten comprises the following steps: S1-1, preparing a BSA solution, an EDC solution, an NHS solution and a homocysteine HCY solution; s1-2, adding an NHS solution and an EDC solution into the BSA solution, uniformly mixing, and oscillating and incubating at 37 DEG C to obtain a mixed solution I; s1-3, preparing a PBS (Phosphate Buffer Solution), and dialyzing the mixed solution at 4 DEG C; s1-4, quantifying and fixing the volume of the dialyzed mixed solution I, adding a homocysteine HCY solution, uniformly mixing, and oscillating and incubating at 37 DEG C to obtain a mixed solution II; and S1-5, dialyzing the mixed solution II at 4 DEG C, and obtaining the human homocysteine hapten after dialysis is finished. And preparing an antibody from the human homocysteine hapten and applying the antibody to preparation of a corresponding detection kit. The kit has high sensitivity and high specificity.

The application provides a method and application for improving DNA yield in vitro amplification, and belongs to the technical field of biology. The application provides a method for improving DNA yield in vitro amplification, which comprises the following steps: (1) reacting a reaction system at 27-38 DEG C for 40-60 min; (2) adding the reaction liquid into a dialysis bag, adding dialysis liquid into an outer tube, and dialyzing at 30-38 DEG C; (3) supplementing tRNA into the reaction liquid for sealing after dialyzing for 1-3 h; the reaction system in step (1) comprises 5x synthesis enzyme mix, 5x synthesis reaction buffer, NTP, dNTP, a template and water; and the concentration of the dNTP is 0.1-0.5 mM. The method can significantly improve the DNA yield in vitro amplification, the concentration of the amplification product is increased by more than about 134 %, and the core problem of insufficient yield in the in vitro DNA amplification is effectively solved.

The application belongs to the technical field of biological medicine, and particularly relates to a cysteine-doxorubicin conjugate as well as a preparation method and application thereof. First, doxorubicin and 3-(2-pyridyl dithio) propionic acid are dissolved in dichloromethane, then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride is added, and after stirring and dissolving at room temperature, heating and stirring reaction is performed. After the reaction is completed, the solvent is spun dry, and the 3-(2-pyridyl dithio) propionic acid doxorubicin ester is obtained through column chromatography separation and purification. Then, the 3-(2-pyridyl dithio) propionic acid doxorubicin ester is dissolved in N,N-dimethylformamide, cysteine is added, and after stirring and dissolving at room temperature, heating and stirring reaction is performed. After the reaction liquid is cooled to room temperature, it is added to a dialysis bag and dialyzed in ultrapure water. After freeze-drying, the cysteine-doxorubicin conjugate is obtained.

The present application relates to the field of synthetic biology, and specifically discloses a preparation method for improving the biological activity of lentinan and application thereof. The method adopts white shiitake mushroom to perform solid-state fermentation on lentinan, and comprises the following steps: S1, grinding the lentinan into powder, then adding appropriate amount of water to stir uniformly, high-pressure sterilization, and cooling to obtain lentinan culture medium; S2, activating white shiitake mushroom strains and preparing white shiitake mushroom seed liquid, adding the white shiitake mushroom seed liquid into the lentinan culture medium according to a certain inoculation amount to perform solid-state fermentation; S3, taking the fermented lentinan culture medium, performing protease enzymolysis first, then adopting ethanol precipitation, centrifuging to take the precipitate, resolubilizing the precipitate, performing dialysis treatment through a dialysis bag, and freeze-drying the dialyzed solution to obtain lentinan. The preparation method significantly improves the yield and purity of lentinan, and at the same time, the antioxidant, anti-inflammatory and pancreatic lipase clearance abilities are significantly improved, and the method has the effect of lubricating intestines.

The invention relates to a preparation method of serum, in particular to a preparation method of human umbilical cord blood serum capable of replacing fetal calf serum. According to the preparation method of the human umbilical cord blood serum capable of replacing the fetal calf serum, CaCl2 is added into anticoagulant umbilical cord blood plasma, prothrombin can be activated into thrombin by calcium ions, the thrombin acts on fibrinogen to be converted into a fibrin polymer, jelly-shaped gel is formed, and finally the blood coagulation process is completed. The purpose of removing fibrinogen in plasma is achieved. Furthermore, the aim of reducing citrate in the sample is achieved through dialysis, and polyethylene glycol is further adopted for concentrating the dialyzed sample, so that the concentration of total protein and cell factors in the umbilical cord serum is increased.

The invention discloses a hot compress device for hemodialysis, which relates to the technical field of medical devices. A device shell is arranged above a base, and installation cavities are symmetrically provided on both sides of the device shell, and a clamping assembly is arranged in the installation cavity; a movement cavity is provided in the middle position of the device shell, a fan is provided in the middle of the top of the movement cavity, and the fan is vertically downwardly arranged in the movement cavity, a film is provided below the fan, and the film is sealed at the bottom of the movement cavity, both ends of the film are fixedly connected to the movement cavity, and a fixing rod is provided in the movement cavity; a mounting block is provided on the fixing rod, and mounting holes are provided on the mounting block, and the number of the mounting holes is the same as that of the fixing rod, and the mounting block is slidably installed with the fixing rod through the mounting holes, and a dressing assembly is provided below the mounting block. The device solves the problem that when hot compress is applied to a wound site after dialysis, the hot compress cannot be evenly fitted to the wound site, resulting in uneven hot compress, thereby increasing the risk of infection at the wound site.

The invention discloses a preparation method of chitosan surface modified extracellular vesicles, which specifically comprises the following steps: S1, dissolving chitosan in water, adjusting the pH value to be acidic, and completely dissolving the chitosan to obtain a chitosan solution with the concentration of 10mg / mL; s2, adding N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride into the solution obtained in the step S1, and uniformly stirring and fully mixing the N-hydroxysuccinimide and the 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride; s3, the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 is dissolved in formamide, and a distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 solution with the concentration being 1 mg / mL is prepared; s4, uniformly mixing the solution obtained in the step S2 and the solution obtained in the step S3, and stirring at room temperature for 12 hours; s5, transferring the mixed solution obtained in the step S4 into a dialysis bag with the specification of 3.5 KDa, and dialyzing in pure water for 24 hours; and S6, co-incubating the dialyzed solution obtained in the step S5 with an extracellular vesicle solution, and self-assembling to form the extracellular vesicle with the chitosan surface modified. The chitosan surface modified extracellular vesicles prepared by the method disclosed by the invention have relatively high encapsulation efficiency on hydrophobic drugs such as fucoxanthin and the like and relatively high stability in various environments. The method is suitable for embedding and delivering various bioactive substances, and has an important application prospect in the aspect of active substance delivery.

The invention discloses a preparation method and application of a coal-based quantum dot nano-material. The preparation method comprises the following steps: S1, sequentially adding a coal-based raw material into a hydrochloric acid solution and a hydrofluoric acid solution for pickling and impurity removal, washing a precipitate to be neutral, and drying to obtain the pickled coal-based raw material; s2, adding the acid-washed coal-based raw material obtained in the step S1 into a reaction container, adding DMF, carrying out ultrasonic treatment, heating to a preset temperature, carrying out a reaction, dropwise adding a hydrogen peroxide solution at the same time, and after the reaction is finished, carrying out self-heating cooling to room temperature so as to obtain a cooled solution; s3, centrifugally separating the cooled solution obtained in the step S2, dialyzing supernate in a dialysis bag, and freeze-drying the dialyzed solution to obtain the coal-based quantum dot nano material. The preparation method of the coal-based quantum dot nano material is simple to operate, high in conversion rate, low in cost and capable of being popularized and applied on a large scale.

The present application provides an enrichment color-developing membrane modified with a fluorine-doped carbon dot material and its application in the detection of three types of perfluorinated compounds. The present application synthesizes the fluorine-doped carbon dot material by a one-pot solvothermal method. 4-diethylamino salicylaldehyde and sodium fluoride are dissolved in deionized water and ultrasonically mixed with phosphoric acid. After the mixture is evenly mixed, it is placed in a polytetrafluoroethylene-lined reactor, and the mixture is heated at 180-220°C for 1-3 hours. After cooling, the resulting solution is centrifuged to remove unreacted products, the upper layer solution is collected, and the sample is placed in a dialysis bag for dialysis for 24-48 hours. The sample is freeze-dried to prepare the fluorine-doped carbon dot material. The fluorine-doped carbon dot material is used as a color developer to achieve the purpose of detecting perfluorinated compounds, and is combined with UiO-66-F4 to prepare an enrichment color-developing membrane, which is convenient for detection and achieves the effect of in-situ monitoring of three types of perfluorinated compounds in water.

The invention belongs to the field of biological medicine, and particularly relates to preparation and application of dextran for relieving ulcerative colitis. The dextran GS128 is formed by directly catalyzing and synthesizing dextransucrase Gtf128 generated by heterologous expression in vitro by taking sucrose as a substrate, and the product is obtained by ethanol precipitation, centrifugation, dissolution and dialysis. The compound shows high anti-digestion performance in the process of simulating gastrointestinal tract digestion, and can relieve the symptoms of ulcerative colitis by inhibiting oxidative stress, adjusting the balance of proinflammatory / anti-inflammatory factors and improving the colon barrier.

The invention discloses a novel application with a cold compress hemostasis function for hemodialysis, and relates to the technical field of medical supplies. The plaster comprises a plaster body, a first opening is formed in the plaster body, internal threads are formed in the upper side of the plaster body and located in the first opening and the inner wall of a mounting head, and bandages used for binding are arranged on the two sides of the plaster body; the hydrocolloid dressing layer is attached to the lower side of the application body. The hydrocolloid dressing layer can be directly and firmly adhered to the skin at the edge of a wound and can absorb seepage, the pressing cylinder is arranged and moves downwards by rotating the pressing cylinder so as to slowly press and stop bleeding of the wound, and the cold insulation ring is arranged so that local blood vessels around the wound can be conveniently constricted. And the traditional Chinese medicine preparation can be immediately used after dialysis, so that the wound can be scientifically nursed, and the pain of a patient can be relieved.

The invention discloses an intelligent dialysis service method and system, and provides an intelligent dialysis service system capable of monitoring physiological information of a patient in real time, judging the dialysis effect, establishing a dialysis model, performing risk assessment and optimizing a treatment scheme. Firstly, physiological information of a patient is monitored in real time through a physiological parameter monitoring module, and data is fed back to a server. The dialysis scheme generation module is used for judging the dialysis effect according to the physiological information after dialysis, generating corresponding dialysis effect description data, establishing a dialysis model based on the generated data, analyzing the physiological information before and after dialysis, obtaining dialysis condition data, generating warning information through the dialysis condition analysis module, and assisting a doctor in judging the state of a patient. According to patient monitoring data analysis and dialysis effect description, abnormal conditions are found in time through an abnormal monitoring and early warning module, risk assessment is carried out, and early warning standards of different levels are set, so that medical staff can carry out corresponding processing.

The invention relates to the technical field of medical nano materials, and particularly discloses a panax notoginseng polysaccharide carbon quantum dot polymer as well as a preparation method and application thereof. The preparation method of the panax notoginseng polysaccharide carbon quantum dot polymer comprises the following steps: S1, extracting panax notoginseng polysaccharide from decoction dregs after total saponins are extracted from panax notoginseng; s2, dispersing panax notoginseng polysaccharide in water according to a ratio of 1g: (10-40) mL, carrying out hydrothermal reaction at 200-300 DEG C for 1-3 hours, and cooling to room temperature; s3, performing ultrasonic treatment, centrifuging to take supernate, filtering, dialyzing and purifying, and dialyzing by using a dialysis bag with the molecular weight cutoff of 500-800D; and S4, freeze-drying the dialyzed solution to obtain the panax notoginseng polysaccharide carbon quantum dot polymer. The medical dressing prepared from the pseudo-ginseng polysaccharide carbon quantum dot polymer provided by the invention is spongy and has a remarkable hemostatic effect, and a mouse liver injury model result shows that the hemostatic time is only about 56s.

The invention relates to the technical field of AKI treatment, in particular to a preparation method and application of nanoparticles, hyaluronic acid, dicyclohexylcarbodiimide and 4-dimethylaminopyridine are taken and dissolved in a dimethyl sulfoxide solution, stirring is performed to form carboxyl of HA, and a reaction system is prepared; adding urolithin A into a reaction system, stirring, adding citric acid and cerium nitrate hexahydrate, pouring an ammonia water solution, and stirring overnight; transferring the solution obtained by stirring overnight into a dialysis bag, and dialyzing to remove water-soluble by-products; centrifuging the dialyzed solution so as to remove water insoluble by-products; and then freeze-drying is carried out, such that the nanoparticles HUCNPs are obtained. The HUCNPs nanoparticles disclosed by the invention can be targeted to damaged kidneys, and compared with the existing urolithin A, the HUCNPs nanoparticles disclosed by the invention have the effects of stronger ROS (reactive oxygen species) removal capability, more effective reduction of inflammatory response of HK-2 cells under stimulation, better apoptosis resistance, better mitochondrial protection effect and the like, and have an unexpected AKI treatment effect.

The present application relates to hemodialysis prescription optimization technical field, especially in a kind of method and device for determining safe and effective prescription interval of hemodialysis.The method is in two-dimensional parameter space with ultrafiltration volume and dialysate sodium concentration as coordinate axis, using double-chamber sodium dynamics model (diffusion equilibrium concentration C_eq=[Na]d / σ (σ=0.97)) to calculate the sodium removal of each parameter combination and serum sodium after dialysis, superimpose sodium removal target line, ultrafiltration rate safety line and serum sodium safety area three constraint boundaries, the area that meets all constraints simultaneously is determined as safe and effective prescription interval.The safe and effective prescription interval is obtained by mathematical model calculation, not determined directly by preset empirical threshold.The present application first proposes the concept of safe and effective prescription interval, upgrades dialysis prescription from single-point empirical selection to interval optimization selection, and the constraint boundary can be dynamically adjusted according to individualized parameters of patients, to provide intuitive multi-constraint prescription decision support tool for clinicians.

The invention discloses a preparation method of a surface modified chitin whisker, which comprises the following steps: taking a proper amount of chitin to carry out acidolysis reaction, removing supernate through a centrifugation method after the reaction is finished, cleaning, then adding the acidolysis chitin into purified water, then adding sodium hydroxide or potassium hydroxide and chloroacetic acid according to a ratio, setting reaction conditions, and carrying out solid-liquid separation to obtain the surface modified chitin whisker. After the reaction is finished, diluting and standing, washing by adopting a centrifugal method to obtain a residual solid, adding water, dialyzing, carrying out ultrasonic treatment, freeze-drying and the like. On the basis of retaining the key structure of the chitin whisker, the surface-modified chitin whisker which is more excellent in dispersity and compatibility than the chitin whisker is simply and efficiently prepared, and meanwhile, a centrifugal-dialysis combined purification technology is matched, so that the reaction period is greatly shortened, the production cost is greatly reduced, and the method is suitable for industrial production. The application prospects of the chitin whiskers in the aspects of beauty makeup, daily chemicals and medicines are greatly improved.

The application belongs to the field of nanomedicine, and discloses a dual-target ROS-responsive nanomedicine for treating MASH and a preparation method thereof, which comprises the following steps: step 1, organic phase preparation, MCC950, Belzutifan and PEG-b-PPS are added into an organic solvent, and ultrasonic treatment is performed until complete dissolution to form an organic phase, the average molecular weight of the PEG block in PEG-b-PPS is 1000-5000, and the polymerization degree of the PPS block is 10-100; step 2, self-assembly wrapping, the organic phase is slowly added into an aqueous phase under constant magnetic stirring, and a primary micelle emulsion is obtained after self-assembly; step 3, dialysis purification and drying, the primary micelle emulsion is transferred into a dialysis bag for dialysis, and then freeze-drying is performed to obtain a dual-target nanomicelle powder. The dual-target ROS-responsive nanomedicine at least comprises the dual-target nanomicelle powder, the medicine breaks through the bottleneck of dissolution and delivery of extremely hydrophobic drugs, has excellent intravenous drug adaptability and stability, can realize long-acting and precise liver targeting enrichment, and greatly reduces the risk of systemic off-target toxicity.

The invention relates to the technical field of medicines, in particular to a nitric oxide-driven nano motor composition as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving cyclodextrin and Boc protected arginine in DMF, and adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing, freeze-drying and separating to obtain a white product CD-Arg; the preparation method comprises the following steps: dissolving rapamycin and thioketal in anhydrous DMF, and adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing, freeze-drying and separating to obtain a yellow product RAPA-TK; the preparation method comprises the following steps: dissolving CD-Arg and RAPA-TK in DMF, and then adding EDC and DMAP; carrying out water bath reaction under a nitrogen condition; dialyzing the product, and freeze-drying and separating to obtain the nano-motor composition. The nano motor provided by the invention has special chemical bonds sensitive and responsive to an inflammatory microenvironment, and is easy to break in the inflammatory environment, so that targeted drug release can be realized.

The invention is applicable to the technical field of biology, and provides a pretreatment method of a protein sample and a peptide diagram analysis method, which comprises the following steps: (a) carrying out a heating denaturation reduction reaction on the protein sample in the presence of a denaturing agent and a reducing agent, and then adding an alkylation reagent to carry out a dark alkylation reaction; (b) filling the reaction liquid into a dialysis bag, and carrying out multiple times of liquid change dialysis; (c) concentrating the dialyzed sample solution, and adding a protease solution to carry out enzymolysis reaction; (d) adding an acid solution into the solution after enzymolysis to terminate the reaction, centrifuging and taking supernate, the method overcomes the problem of loss of a membrane adsorption sample caused by a traditional ultrafiltration method, is particularly suitable for pretreatment of a trace protein sample, reduces the use amount of the sample and the experiment cost, can be compatible with synchronous digestion reaction of multiple enzymes, and has a wide application prospect. The pretreated sample is analyzed by LC-MS / MS, a chromatogram with good peak shape and good separation degree can be obtained, and reliable data is provided for quality control and structural identification of protein drugs.

The present invention discloses a preparation method and application of artificial peroxisomes LOX-Ce6-Mn; belonging to the field of nanomaterials and tumor treatment technology, the preparation steps are: firstly uniformly mixing prepared DCC, NHS, Ce6, and LOX in proportion, dialyzing with a dialysis bag after the reaction, adding an aqueous solution of MnCl2, adjusting the pH value, dialyzing, and drying to obtain LCM nanoparticles. In the present invention, the lactate oxidase activity, oxidase activity (OXD), peroxidase activity (POD), and catalase activity (CAT) of the LCM nanoparticles can constitute a cascade reaction, causing tumor cells to produce efficient reactive oxygen and consume intratumoral lactate and glutathione, causing tumor cells to undergo autophagy and apoptosis; in addition, the H2O2 catalyzed by LOX can be transported by Mn 2+ The mediated Fenton-like reaction converts hydroxyl radicals into highly toxic free radicals, further amplifying the oxidative damage of cancer cells. LCM nanoparticles are tumor-specific in killing tumor cells, effectively accumulating in tumor sites, with little killing effect on normal sites, and have good biocompatibility.

The invention discloses a nano material for inhibiting aseptic inflammation as well as a preparation method and application of the nano material. The nano material is hybrid liposome nano vesicles modified by a platelet membrane, and the mass ratio of protein to lipid is 1: 2. The nano vesicles are loaded with sylvite compounds, can target in-vivo tissue injury parts, release potassium ions under the action of ultrasound, realize intracellular pH regulation and control through hydrogen-potassium exchange, and inhibit aseptic inflammation. The hybrid liposome is formed by mixing phospholipid and cholesterol, and the potassium salt compound is potassium carbonate or potassium bicarbonate. The preparation method comprises the following steps: preparing a hybrid liposome membrane and a platelet membrane, respectively adding a sylvite compound solution to carry out ultrasonic hydration and resuspension washing, preparing hybrid liposome vesicles and platelet membrane vesicles entrapped with sylvite compounds, and carrying out ultrasonic treatment, size stabilization and dialysis to obtain the platelet membrane modified hybrid liposome nano-vesicles. Inflammation can be inhibited in the early stage of injury, and side effects of traditional drugs are avoided.

The invention relates to the technical field of medical instruments, and provides a dialysis urea clearance rate online monitoring method, a computer system and a monitoring system.The monitoring method comprises the steps that S1, a detection light source is adopted to irradiate dialysis waste liquid, and the incident light intensity I0 is recorded; s2, collecting the initial transmission light intensity I1 penetrating through the dialysis waste liquid and the transmission light intensity It at the current t moment, and calculating the ratio Rt of the urea nitrogen concentration after dialysis to the urea nitrogen concentration before dialysis on the basis of I1 and It; and S3, calculating a urea clearance index Kt / Vt value at the current t moment based on the Rt. The method has the advantages that the final urea clearance index Kt / Vt value is obtained according to the data of the illumination intensity of the dialysis waste liquid, real-time monitoring of urea data is achieved, the accuracy of the final urea clearance rate calculation result is improved, medical staff can conveniently and timely obtain the urea clearance condition of a dialyzer on blood, and the medical staff can conveniently and rapidly know the urea clearance condition of the dialyzer on the blood. In addition, invasive operation in a traditional blood sampling measurement mode is avoided, discomfort brought to a patient is avoided, and the infection risk is reduced.