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Extraction and separation method of phyllanthus urinaria polyose

A separation method and bead polysaccharide technology, applied in the field of medicine, can solve the problem of few polysaccharide researches

Inactive Publication Date: 2013-04-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Phyllanthus has anti-hepatitis B virus, liver protection, anti-tumor effects, etc. It has been generally confirmed, and relevant scholars have also studied the structure and biological activity of several chemical components, such as gallic acid, flavonoids, and phyllocin. research, but little research has been done on the polysaccharides

Method used

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  • Extraction and separation method of phyllanthus urinaria polyose
  • Extraction and separation method of phyllanthus urinaria polyose
  • Extraction and separation method of phyllanthus urinaria polyose

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Embodiment Construction

[0026] Below in conjunction with embodiment the present invention is described in detail:

[0027] Extraction of Phyllostachys polysaccharide

[0028] The whole plant of Phyllostachys phylloxera was washed with distilled water to remove the soil, dried at 50°C, crushed, and sealed for later use. Its powder is yellow-green.

[0029] Petroleum ether reflux at 80 °C to degrease → 95% ethanol at 80 °C to remove small molecule sugars → 90 °C water to reflux for three times, combine the filtrate for three times and centrifuge at 4000r / min, concentrate to 1 / 4 volume → add 4 times the volume of 95% ethanol , stand still for 24 hours, centrifuge, redissolve in distilled water → remove protein by sevag method (chloroform: n-butanol = 4:1, polysaccharide solution: mixed solution = 3:1), magnetically stir for 30 minutes, fully stand for stratification, repeat 7~ Free protein can be removed after 8 operations → add 4 times the volume of 95% ethanol to the polysaccharide solution after ...

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Abstract

The invention discloses an extraction and separation method of phyllanthus urinaria polyose PULP III, which comprises the following steps of removing dust by washing a whole phyllanthus urinaria plant with distilled water, drying, grinding, degreasing with petroleum ether, removing micromolecular sugar with ethanol, leaching medicine dregs with water, conducting centrifugal separation on leached liquid, concentrating filtrate, conducting ethanol precipitation with ethanol, conducting deproteinization, washing, purification and dialysis on an ethanol precipitate, drying, obtaining phyllanthus urinaria fine polyose, conducting deproteinization and dialysis, treating with an ion exchange column, eluting with appropriate NaCl gradient solutions in sequence, conducting track detection by a phenol-sulfuric acid method, obtaining an elution curve, collecting a third main peak component, concentrating, dialysing with redistilled water for 48h, freeze-drying, and obtaining a pure phyllanthus urinaria polyose PULP III component. According to the method, the pure and unique phyllanthus urinaria polyose component is obtained by separating and purifying crude phyllanthus urinaria polyose.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for extracting and separating phyllophyllum polysaccharide PULPIII. Background technique [0002] Phyllostachys ( Phyllanthus urinaria L . ), also known as pearl grass, night grass, yin and yang grass, belongs to Euphorbiaceae ( Euphorbi aceae ) The dry whole plant of Phyllostachys phylloxera is widely used in the folks for diuresis and detoxification, calming the liver and clearing away heat. In 1988, Indian scholar Thyagarajan et al. [2] For the first time, it has been proved through experiments that bitter-flavored Phyllostachys phylloxera can make 59% of patients' hepatitis B surface antigen (HBsAg) negative. This experimental result has aroused the attention of scholars at home and abroad. Numerous studies have shown ] Phyllanthus phylloxera has the effects of anti-hepatitis B virus, liver protection and enzyme reduction, anti-hepatic fibrosis, prevention ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 李清禄李宇翔李凌峰张丽丽谢勇平周学酬黄志坚
Owner FUJIAN AGRI & FORESTRY UNIV
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