Adipose-derived stem cell composition for wound repair

A technology of fat stem cells and wound repair, applied in animal cells, drug combinations, vertebrate cells, etc., can solve the problems of poor effect and poor inhibition of scar production
CN111282018AActive Publication Date: 2020-06-16优赛生命科学发展有限公司

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
优赛生命科学发展有限公司
Publication Date
2020-06-16
Patent Text Reader

Abstract

The invention discloses an adipose-derived stem cell composition for wound repair. The composition is characterized by consisting of an adipose-derived stem cell culture product which is used as an active ingredient and accounts for 1-2 percent by weight, and at least one medicinal auxiliary material which can be externally used, wherein the adipose-derived stem cell culture product is prepared bythe following method: A) inoculating adipose-derived mesenchymal stem cells in a culture medium, b) putting the culture dish inoculated with the adipose-derived mesenchymal stem cells into 10-15% ofCO2; and C) after the culture is completed, removing the culture medium, collecting cells, cleaning the cells, homogenizing the cells, centrifuging the homogenate, taking the supernatant, putting thesupernatant into a dialysis bag, dialyzing for 8-24 hours by using PBS at 4 DEG C, and freeze-drying the solution in the dialysis bag to obtain the adipose-derived stem cell culture product.
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Description

technical field

[0001] The present invention relates to a composition for external use. Background technique

[0002] Adipose-derived stem cells (ASCs) were isolated from adipose tissue for the first time in 2001, because they have the same high proliferation and self-renewal ability as bone marrow-derived stem cells (BMSCs) , a large number of ASCs can be isolated from adipose tissue, and can differentiate into mesoderm and non-mesoderm cells, making it a hot spot in the application of adult stem cells in regenerative medicine, and is widely used in skin tissue regeneration, cartilage and bone Tissue regeneration, wound healing and many other research fields.

[0003] In the study of wound repair, how to suppress or even eliminate the generation of scar is a difficult problem in existing clinical medicine. Chinese patent CN107320768A discloses an auxiliary material for wound repair containing induced pluripotent stem cell extract, which utilizes the ability of induced plu...

Claims

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