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Adipose-derived stem cell composition for wound repair

A technology of fat stem cells and wound repair, applied in animal cells, drug combinations, vertebrate cells, etc., can solve the problems of poor effect and poor inhibition of scar production

Active Publication Date: 2020-06-16
优赛生命科学发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN107320768A discloses an auxiliary material for wound repair containing induced pluripotent stem cell extract, which utilizes the ability of induced pluripotent stem cell extract to induce transformation of fibroblasts, and its main effect is to promote wound repair And antibacterial, but its effect on severe skin wounds and burns is poor, especially in the repair process of severe wounds, the inhibitory effect on scar production is poor

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0014] 1. The preparation of adipose stem cell culture products adopts the following methods:

[0015] A) Add adipose-derived mesenchymal stem cells to 5×10 4 The inoculation amount of / ml is inoculated in the medium, and described medium is the basal medium containing induction culture reagent, and described basal medium is the low-sugar DMEM medium containing 10% volume fraction fetal bovine serum, and described induction culture reagent dexamethasone, diammonium glycyrrhizinate, vitamin D 3 and retinoic acid, the amount of induction culture reagent in the culture medium is: dexamethasone (A 1 ) μmol / L, diammonium glycyrrhizinate (A 2 )μmol / L, Vitamin D 3 (A 3 ) μmol / L, retinoic acid (A 4 ) 1~3μmol / L;

[0016] B) Place the culture dish inoculated with adipose-derived mesenchymal stem cells on B 1 % concentration of CO 2 , Cultivate under the condition of 37°C, replace the medium every 3 days; co-cultivate for 7 days;

[0017] C) After the culture is completed, remov...

preparation Embodiment

[0036] The composition is prepared as a liquid dressing, prepared as follows:

[0037] 1) Dissolve the adipose stem cell culture product in water, add the prescribed amount of hydroxypropyl methylcellulose to fully dissolve, and obtain liquid A;

[0038]2) Dissolve the prescribed amount of chitosan in acetic acid solution to obtain chitosan acetic acid solution, slowly add liquid A into the chitosan acetic acid solution, stir evenly, adjust the pH to 6.5~6.8 with 0.1M NaOH solution, and make up the remaining Amount of water, that is.

[0039] The formula of preparation embodiment sees the following table (unit: weight percent %)

[0040] serial number Adipose stem cell culture product / dosage Chitosan dosage / % HPMC dosage / % 1 Example 1 / 1% 1.5 0.5 2 Example 2 / 2% 2.5 1 3 Example 3 / 1% 2 0.5 4 Example 4 / 2% 1.5 1 5 Example 5 / 2% 2.5 0.5 6 Example 6 / 1% 2 1

[0041] The composition obtained in the preparation example ...

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PUM

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Abstract

The invention discloses an adipose-derived stem cell composition for wound repair. The composition is characterized by consisting of an adipose-derived stem cell culture product which is used as an active ingredient and accounts for 1-2 percent by weight, and at least one medicinal auxiliary material which can be externally used, wherein the adipose-derived stem cell culture product is prepared bythe following method: A) inoculating adipose-derived mesenchymal stem cells in a culture medium, b) putting the culture dish inoculated with the adipose-derived mesenchymal stem cells into 10-15% ofCO2; and C) after the culture is completed, removing the culture medium, collecting cells, cleaning the cells, homogenizing the cells, centrifuging the homogenate, taking the supernatant, putting thesupernatant into a dialysis bag, dialyzing for 8-24 hours by using PBS at 4 DEG C, and freeze-drying the solution in the dialysis bag to obtain the adipose-derived stem cell culture product.

Description

technical field [0001] The present invention relates to a composition for external use. Background technique [0002] Adipose-derived stem cells (ASCs) were isolated from adipose tissue for the first time in 2001, because they have the same high proliferation and self-renewal ability as bone marrow-derived stem cells (BMSCs) , a large number of ASCs can be isolated from adipose tissue, and can differentiate into mesoderm and non-mesoderm cells, making it a hot spot in the application of adult stem cells in regenerative medicine, and is widely used in skin tissue regeneration, cartilage and bone Tissue regeneration, wound healing and many other research fields. [0003] In the study of wound repair, how to suppress or even eliminate the generation of scar is a difficult problem in existing clinical medicine. Chinese patent CN107320768A discloses an auxiliary material for wound repair containing induced pluripotent stem cell extract, which utilizes the ability of induced plu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00A61K9/06A61K35/28A61K47/36A61K47/38A61P17/02C12N5/0775
CPCA61K9/06A61K35/28A61K47/36A61K47/38A61L26/0023A61L26/0057A61L26/0066A61L26/008A61L2300/30A61L2300/412A61P17/02C12N5/0667C12N2500/38C12N2501/39C12N2501/999C08L5/08C08L1/284
Inventor 陈晓波张健韩洪起冯春玲
Owner 优赛生命科学发展有限公司
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